Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Swerdlow, R.D.; Setlow, P.
    Purification and characterization of a Bacillus megaterium disulfide reductase specific for disulfides containing pantethine 4',4"-diphosphate (1983), J. Bacteriol., 153, 475-484.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.8 arsenite
-
 Priestia megaterium
1.8.1.8 N-ethylmaleimide
-
 Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.8 0.65
-
 pantethine 4',4''-diphosphate
-
 Priestia megaterium
1.8.1.8 7.4
-
 CoA-SS-CoA
-
 Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.8 55000
-
 2 * 55000, SDS-PAGE Priestia megaterium
1.8.1.8 122000
-
 gel filtration Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.8 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.8
-
 Priestia megaterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.8 cell culture the enzyme is at a low level in log-phase cells but increases up to 10fold early in the stationary phase and has a similar specific activity in both the mother cell and the forespore compartment. The enzyme activity falls only slowly during spore germination and outgrowth Priestia megaterium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.8 additional information the enzyme is active only on disulfides containing pantethine 4',4"-diphosphate moieties, including pantethine 4',4"-diphosphate, oxidized coenzyme A, and coenzyme A in disulfide linkage to acyl carrier protein Priestia megaterium ?
-
 ?
1.8.1.8 NADH + CoA-SS-CoA + H+
-
 Priestia megaterium NAD+ + CoA
-
 ?
1.8.1.8 NADH + pantethine 4',4''-diphosphate
-
 Priestia megaterium NAD+ + 4'-phosphopantetheine
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.8 dimer 2 * 55000, SDS-PAGE Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
1.8.1.8 NADH-linked disulfide reductase
-
 Priestia megaterium
1.8.1.8 panthethine 4'4-diphosphate-specific reductase
-
 Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.8 6.8
-
 substrate: pantethine 4',4''-diphosphate Priestia megaterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.1.8 additional information
-
 seven times as active in phosphate buffer as in Tris at the same pH Priestia megaterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.8 FAD contains one molecule of flavin adenine dinucleotide per subunit Priestia megaterium