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Literature summary extracted from

  • Otieno, D.O.; Shah, N.P.
    Endogenous beta-glucosidase and beta-galactosidase activities from selected probiotic micro-organisms and their role in isoflavone biotransformation in soymilk (2007), J. Appl. Microbiol., 103, 910-917.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.21 nutrition the enzyme is important in hydrolysis of the predominant isoflavone glycosides into isoflavone aglycones in order to improve biological activity of soymilk, overview Lacticaseibacillus casei
3.2.1.21 nutrition the enzyme is important in hydrolysis of the predominant isoflavone glycosides into isoflavone aglycones in order to improve biological activity of soymilk, overview Lactobacillus acidophilus
3.2.1.21 nutrition the enzyme is important in hydrolysis of the predominant isoflavone glycosides into isoflavone aglycones in order to improve biological activity of soymilk, overview Bifidobacterium animalis
3.2.1.23 nutrition the enzyme is important in hydrolysis of the predominant isoflavone glycosides into isoflavone aglycones in order to improve biological activity of soymilk, overview Lacticaseibacillus casei
3.2.1.23 nutrition the enzyme is important in hydrolysis of the predominant isoflavone glycosides into isoflavone aglycones in order to improve biological activity of soymilk, overview Lactobacillus acidophilus
3.2.1.23 nutrition the enzyme is important in hydrolysis of the predominant isoflavone glycosides into isoflavone aglycones in order to improve biological activity of soymilk, overview Bifidobacterium animalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Bifidobacterium animalis
-
 ssp. lactis, strain Bb12
-
3.2.1.21 Lacticaseibacillus casei
-
-
-
3.2.1.21 Lacticaseibacillus casei 2607
-
-
-
3.2.1.21 Lactobacillus acidophilus
-
 strain ATCC 4461
-
3.2.1.23 Bifidobacterium animalis
-
 ssp. lactis, strain Bb12
-
3.2.1.23 Lacticaseibacillus casei
-
-
-
3.2.1.23 Lacticaseibacillus casei 2607
-
-
-
3.2.1.23 Lactobacillus acidophilus
-
 strain ATCC 4461
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 additional information organism in soymilk Lacticaseibacillus casei
-
3.2.1.21 additional information organism in soymilk Lactobacillus acidophilus
-
3.2.1.21 additional information organism in soymilk Bifidobacterium animalis
-
3.2.1.23 additional information organism in soymilk Lacticaseibacillus casei
-
3.2.1.23 additional information organism in soymilk Lactobacillus acidophilus
-
3.2.1.23 additional information organism in soymilk Bifidobacterium animalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 additional information
-
 detection of enzyme activity and concentration of isoflavone aglycones during fermentation of soymilk, overview Lacticaseibacillus casei
3.2.1.21 additional information
-
 detection of enzyme activity and concentration of isoflavone aglycones during fermentation of soymilk, overview Lactobacillus acidophilus
3.2.1.21 additional information
-
 detection of enzyme activity and concentration of isoflavone aglycones during fermentation of soymilk, overview Bifidobacterium animalis
3.2.1.23 additional information
-
 detection of enzyme activity and concentration of isoflavone aglycones during fermentation of soymilk, overview Lacticaseibacillus casei
3.2.1.23 additional information
-
 detection of enzyme activity and concentration of isoflavone aglycones during fermentation of soymilk, overview Lactobacillus acidophilus
3.2.1.23 additional information
-
 detection of enzyme activity and concentration of isoflavone aglycones during fermentation of soymilk, overview Bifidobacterium animalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Lacticaseibacillus casei 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Lactobacillus acidophilus 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Bifidobacterium animalis 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Lacticaseibacillus casei 2607 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Lacticaseibacillus casei 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Lactobacillus acidophilus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium animalis 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Lacticaseibacillus casei 2607 2-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 37
-
 in vivo fermentation temperature and assay at Lacticaseibacillus casei
3.2.1.21 37
-
 in vivo fermentation temperature and assay at Lactobacillus acidophilus
3.2.1.21 37
-
 in vivo fermentation temperature and assay at Bifidobacterium animalis
3.2.1.23 37
-
 in vivo fermentation temperature and assay at Lacticaseibacillus casei
3.2.1.23 37
-
 in vivo fermentation temperature and assay at Lactobacillus acidophilus
3.2.1.23 37
-
 in vivo fermentation temperature and assay at Bifidobacterium animalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.7
-
 in vivo fermentation pH Lacticaseibacillus casei
3.2.1.21 6.7
-
 in vivo fermentation pH Lactobacillus acidophilus
3.2.1.21 6.7
-
 in vivo fermentation pH Bifidobacterium animalis
3.2.1.21 7
-
 assay at Lacticaseibacillus casei
3.2.1.21 7
-
 assay at Lactobacillus acidophilus
3.2.1.21 7
-
 assay at Bifidobacterium animalis
3.2.1.23 6.7
-
 in vivo fermentation pH Lacticaseibacillus casei
3.2.1.23 6.7
-
 in vivo fermentation pH Lactobacillus acidophilus
3.2.1.23 6.7
-
 in vivo fermentation pH Bifidobacterium animalis
3.2.1.23 6.8
-
 assay at Lacticaseibacillus casei
3.2.1.23 6.8
-
 assay at Lactobacillus acidophilus
3.2.1.23 6.8
-
 assay at Bifidobacterium animalis