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Literature summary extracted from

  • Chen, J.; Zhang, Y.Q.; Zhao, C.Q.; Li, A.N.; Zhou, Q.X.; Li, D.C.
    Cloning of a gene encoding thermostable glucoamylase from Chaetomium thermophilum and its expression in Pichia pastoris (2007), J. Appl. Microbiol., 103, 2277-2284.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 gene gla, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, functional overexpression in Pichia pastoris strain GS115, secretion to the culture supernatant Thermochaetoides thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 66000
-
 x * 66000, recombinant enzyme, SDS-PAGE Thermochaetoides thermophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Thermochaetoides thermophila Q0H9W2 a soil-borne thermophilic fungus, gene gla
-
3.2.1.3 Thermochaetoides thermophila CT2 Q0H9W2 a soil-borne thermophilic fungus, gene gla
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein
-
 Thermochaetoides thermophila

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 recombinant enzyme from Pichia pastoris strain GS115 culture supernatant Thermochaetoides thermophila

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.3 mycelium
-
 Thermochaetoides thermophila
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 16.73
-
 recombinant enzyme in Pichia pastoris Thermochaetoides thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O soluble starch Thermochaetoides thermophila beta-D-glucose + ?
-
 ?
3.2.1.3 starch + H2O soluble starch Thermochaetoides thermophila CT2 beta-D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 66000, recombinant enzyme, SDS-PAGE Thermochaetoides thermophila

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Thermochaetoides thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 65
-
-
 Thermochaetoides thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 60
-
 purified recombinant enzyme, stable Thermochaetoides thermophila
3.2.1.3 70
-
 purified recombinant enzyme, 60 min, 80% remaining activity Thermochaetoides thermophila
3.2.1.3 80
-
 purified recombinant enzyme, half-life is 40 min Thermochaetoides thermophila
3.2.1.3 90
-
 purified recombinant enzyme, half-life is 10 min Thermochaetoides thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.5 5
-
 Thermochaetoides thermophila