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Literature summary extracted from

  • Yaoi, K.; Hiyoshi, A.; Mitsuishi, Y.
    Screening, purification and characterization of a prokaryotic isoprimeverose-producing oligoxyloglucan hydrolase from Oerskovia sp. Y1 (2007), J. Appl. Glycosci., 54, 91-94.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.120 0.7
-
 Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
-
 Oerskovia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.120 105000
-
 x * 105000, SDS-PAGE Oerskovia sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.120 Oerskovia sp.
-
 Y1
-
3.2.1.120 Oerskovia sp. Y1
-
 Y1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.120 by anion-exchange chromatography and gel filtration Oerskovia sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.120 85
-
 with Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc Oerskovia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.120 (Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
-
 Oerskovia sp. beta-D-xylose + Xylbeta(1-6)Glcbeta(1-4)Glc
-
 ?
3.2.1.120 (Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
-
 Oerskovia sp. Y1 beta-D-xylose + Xylbeta(1-6)Glcbeta(1-4)Glc
-
 ?
3.2.1.120 Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
-
 Oerskovia sp. beta-D-xylose + (Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
-
 ?
3.2.1.120 Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
-
 Oerskovia sp. Y1 beta-D-xylose + (Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
-
 ?
3.2.1.120 Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + isoprimeverose
-
 Oerskovia sp. Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
-
 ?
3.2.1.120 Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + isoprimeverose
-
 Oerskovia sp. Y1 Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
-
 ?
3.2.1.120 Xylbeta(1-6)Glcbeta(1-4)Glc + H2O
-
 Oerskovia sp. Xylbeta(1-6)Glc + beta-D-glucopyranose
-
 ?
3.2.1.120 Xylbeta(1-6)Glcbeta(1-4)Glc + H2O
-
 Oerskovia sp. Y1 Xylbeta(1-6)Glc + beta-D-glucopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.120 ? x * 105000, SDS-PAGE Oerskovia sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.120 IPase
-
 Oerskovia sp.
3.2.1.120 isoprimeverose-producing oligoxyloglucan hydrolase
-
 Oerskovia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.120 55
-
-
 Oerskovia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.120 45
-
 more than 90% of the enzyme activity retained Oerskovia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.120 4.5
-
-
 Oerskovia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.120 3.5 7.5
-
 Oerskovia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.120 3.5 7.5
-
 Oerskovia sp.