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Literature summary extracted from
- Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase (2006), J. Antibiot., 59, 471-479.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.99.16 | expression in Escherichia coli | Streptomyces avermitilis |
| 4.2.3.22 | for construction of a geoA-deletion mutant of Streptomyces avermitilis, and for expression in Escherichia coli BL21DE3 cells | Streptomyces avermitilis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.99.16 | 0.000075 | - |
(2E,6E)-farnesyl diphosphate | pH 8.2, 30°C | Streptomyces avermitilis |  |
| 4.2.3.22 | 0.000075 | - |
farnesyl diphosphate | - |
Streptomyces avermitilis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.16 | Co2+ | 10-20% of the activity with Mg2+ | Streptomyces avermitilis | |
| 4.1.99.16 | Cu2+ | 10-20% of the activity with Mg2+ | Streptomyces avermitilis | |
| 4.1.99.16 | Fe2+ | 50% of the activity with Mg2+ | Streptomyces avermitilis | |
| 4.1.99.16 | Mg2+ | preferred divalent cation, maximum activity at 10 mM | Streptomyces avermitilis | |
| 4.1.99.16 | Mn2+ | 10-20% of the activity with Mg2+ | Streptomyces avermitilis | |
| 4.1.99.16 | additional information | absolute requirement for a divalent cation. No activity with Ni2+ or Ca2+ | Streptomyces avermitilis | |
| 4.1.99.16 | Zn2+ | 50% of the activity with Mg2+ | Streptomyces avermitilis | |
| 4.2.3.22 | Co2+ | 10-20% activity, compared to reaction with Mg2+ | Streptomyces avermitilis | |
| 4.2.3.22 | Cu2+ | 10-20% activity, compared to reaction with Mg2+ | Streptomyces avermitilis | |
| 4.2.3.22 | Fe2+ | 50% activity, compared to reaction with Mg2+ | Streptomyces avermitilis | |
| 4.2.3.22 | Mg2+ | 100% activity | Streptomyces avermitilis | |
| 4.2.3.22 | Mn2+ | 10-20% activity, compared to reaction with Mg2+ | Streptomyces avermitilis | |
| 4.2.3.22 | Zn2+ | 50% activity, compared to reaction with Mg2+ | Streptomyces avermitilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.22 | 83000 | - |
determined by SDS-PAGE | Streptomyces avermitilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.16 | Streptomyces avermitilis | Q82L49 | germacradienol/geosmin synthase | - |
| 4.2.3.22 | Streptomyces avermitilis | Q82L49 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.3.22 | using Q-Sepharose resin and a Q-Sepharose column | Streptomyces avermitilis |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 4.2.3.22 | resuspended inclusion body protein is refolded | Streptomyces avermitilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.16 | (2E,6E)-farnesyl diphosphate + H2O | overall reaction of germacradienol/geosmin synthase | Streptomyces avermitilis | (4S,7R)-germacra-1(10)E,5E-diene-11-ol + (7S)-germacrene D + geosmin + ? | presence of Mg2+, synthesis of 66% (4S,7R)-germacra-1(10)E,5E-diene-11-ol, 24% (7S)-germacrene D, 8% geosmin, and 2% of a hydrocarbon, tentatively assigned the structure of octalin | ? | |
| 4.1.99.16 | additional information | nerolidyl diphosphate is excluded as an intermediate in the enzymatic formation of germacradienol, germacrene D, and geosmin | Streptomyces avermitilis | ? | - |
? | |
| 4.2.3.22 | (2E,6E)-farnesyl diphosphate + H2O | - |
Streptomyces avermitilis | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | 66% | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.99.16 | geoA | - |
Streptomyces avermitilis |
| 4.2.3.22 | germacradienol/germacrene D synthase | - |
Streptomyces avermitilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.22 | 30 | - |
activity assay | Streptomyces avermitilis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.99.16 | 0.0031 | - |
(2E,6E)-farnesyl diphosphate | pH 8.2, 30°C | Streptomyces avermitilis | |
| 4.2.3.22 | 0.0031 | - |
farnesyl diphosphate | - |
Streptomyces avermitilis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.16 | 7.6 | 9 | - |
Streptomyces avermitilis |
| 4.2.3.22 | 8.2 | - |
activity assay | Streptomyces avermitilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.22 | 7.6 | 9 | - |
Streptomyces avermitilis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.1.99.16 | physiological function | mutants with a deleted geoA are unable to produce either germacradienol or geosmin | Streptomyces avermitilis |
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