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Literature summary extracted from

  • Tesic, M.; Wicki, J.; Poon, D.K.; Withers, S.G.; Douglas, D.J.
    Gas phase noncovalent protein complexes that retain solution binding properties: Binding of xylobiose inhibitors to the beta-1, 4 exoglucanase from Cellulomonas fimi (2007), J. Am. Soc. Mass Spectrom., 18, 64-73.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.74 analysis tandem mass spectrometry to compare gas-phase and solution binding of small-molecule inhibitors to the wild type and three mutant forms of Cex Cellulomonas fimi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.74 expression in Escherichia coli Cellulomonas fimi

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.74 N44A based on the pUC12-1.1Cex PTIS plasmid Cellulomonas fimi
3.2.1.74 Q87M based on the pUC12-1.1Cex PTIS plasmid Cellulomonas fimi
3.2.1.74 Q87Y based on the pUC12-1.1Cex PTIS plasmid Cellulomonas fimi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.74 xylobiosyl-deoxynojirimycin X2DNJ Cellulomonas fimi
3.2.1.74 xylobiosyl-isofagomine X2IF Cellulomonas fimi
3.2.1.74 xylobiosyl-isofagomine lactam X2IL Cellulomonas fimi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.74 Cellulomonas fimi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.74 catalytic and cellulose binding domains by cellulose FPLC column chromatography, ultrafiltration and papain treatment Cellulomonas fimi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.74 beta-1,4-xylan + H2O
-
Cellulomonas fimi ?
-
?
3.2.1.74 xylan + H2O
-
Cellulomonas fimi ?
-
?
3.2.1.74 xylo-oligosaccharides + H2O
-
Cellulomonas fimi ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.74 Cex
-
Cellulomonas fimi
3.2.1.74 glucan 1,4-beta-glucosidase
-
Cellulomonas fimi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.74 0.00013
-
xylobiosyl-isofagomine wild type Cellulomonas fimi
3.2.1.74 0.00034
-
xylobiosyl-isofagomine lactam wild type Cellulomonas fimi
3.2.1.74 0.00039
-
xylobiosyl-isofagomine Q87M mutant Cellulomonas fimi
3.2.1.74 0.0012
-
xylobiosyl-isofagomine Q87Y mutant Cellulomonas fimi
3.2.1.74 0.0021
-
xylobiosyl-isofagomine lactam Q87M mutant Cellulomonas fimi
3.2.1.74 0.0058
-
xylobiosyl-deoxynojirimycin wild type Cellulomonas fimi
3.2.1.74 0.0071
-
xylobiosyl-isofagomine N44A mutant Cellulomonas fimi
3.2.1.74 0.011
-
xylobiosyl-isofagomine lactam Q87Y mutant Cellulomonas fimi
3.2.1.74 0.018
-
xylobiosyl-deoxynojirimycin Q87M mutant Cellulomonas fimi
3.2.1.74 0.024
-
xylobiosyl-isofagomine lactam N44A mutant Cellulomonas fimi
3.2.1.74 0.062
-
xylobiosyl-deoxynojirimycin Q87Y mutant Cellulomonas fimi
3.2.1.74 0.18
-
xylobiosyl-deoxynojirimycin N44A mutant Cellulomonas fimi