EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.74 | analysis | tandem mass spectrometry to compare gas-phase and solution binding of small-molecule inhibitors to the wild type and three mutant forms of Cex | Cellulomonas fimi |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.74 | expression in Escherichia coli | Cellulomonas fimi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.74 | N44A | based on the pUC12-1.1Cex PTIS plasmid | Cellulomonas fimi |
3.2.1.74 | Q87M | based on the pUC12-1.1Cex PTIS plasmid | Cellulomonas fimi |
3.2.1.74 | Q87Y | based on the pUC12-1.1Cex PTIS plasmid | Cellulomonas fimi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.74 | xylobiosyl-deoxynojirimycin | X2DNJ | Cellulomonas fimi | |
3.2.1.74 | xylobiosyl-isofagomine | X2IF | Cellulomonas fimi | |
3.2.1.74 | xylobiosyl-isofagomine lactam | X2IL | Cellulomonas fimi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.74 | Cellulomonas fimi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.74 | catalytic and cellulose binding domains by cellulose FPLC column chromatography, ultrafiltration and papain treatment | Cellulomonas fimi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.74 | beta-1,4-xylan + H2O | - |
Cellulomonas fimi | ? | - |
? | |
3.2.1.74 | xylan + H2O | - |
Cellulomonas fimi | ? | - |
? | |
3.2.1.74 | xylo-oligosaccharides + H2O | - |
Cellulomonas fimi | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.74 | Cex | - |
Cellulomonas fimi |
3.2.1.74 | glucan 1,4-beta-glucosidase | - |
Cellulomonas fimi |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.74 | 0.00013 | - |
xylobiosyl-isofagomine | wild type | Cellulomonas fimi | |
3.2.1.74 | 0.00034 | - |
xylobiosyl-isofagomine lactam | wild type | Cellulomonas fimi | |
3.2.1.74 | 0.00039 | - |
xylobiosyl-isofagomine | Q87M mutant | Cellulomonas fimi | |
3.2.1.74 | 0.0012 | - |
xylobiosyl-isofagomine | Q87Y mutant | Cellulomonas fimi | |
3.2.1.74 | 0.0021 | - |
xylobiosyl-isofagomine lactam | Q87M mutant | Cellulomonas fimi | |
3.2.1.74 | 0.0058 | - |
xylobiosyl-deoxynojirimycin | wild type | Cellulomonas fimi | |
3.2.1.74 | 0.0071 | - |
xylobiosyl-isofagomine | N44A mutant | Cellulomonas fimi | |
3.2.1.74 | 0.011 | - |
xylobiosyl-isofagomine lactam | Q87Y mutant | Cellulomonas fimi | |
3.2.1.74 | 0.018 | - |
xylobiosyl-deoxynojirimycin | Q87M mutant | Cellulomonas fimi | |
3.2.1.74 | 0.024 | - |
xylobiosyl-isofagomine lactam | N44A mutant | Cellulomonas fimi | |
3.2.1.74 | 0.062 | - |
xylobiosyl-deoxynojirimycin | Q87Y mutant | Cellulomonas fimi | |
3.2.1.74 | 0.18 | - |
xylobiosyl-deoxynojirimycin | N44A mutant | Cellulomonas fimi |