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Literature summary extracted from

  • Ran, N.; Frost, J.W.
    Directed evolution of 2-keto-3-deoxy-6-phosphogalactonate aldolase to replace 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase (2007), J. Am. Chem. Soc., 129, 6130-6139.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.21 expressed in Escherichia coli strain NR7 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.54 additional information KDPGal aldolase mutant EC03-1 (F33I/D58N/Q72H/A75V/V85A/V154F) develops increased DAHP synthase activity Escherichia coli
4.1.2.21 F33I/D58N/Q72H/A75V/V85A/V154F higher activity compared to the wild type enzyme Escherichia coli
4.1.2.21 additional information mutant NR8.276-2 exhibits a 60fold improvement in the ratio kcat/KM relative to that of the wild type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.21 0.12
-
 D-erythrose 4-phosphate mutant enzyme F33I/D58N/Q72H/A75V/V85A/V154F, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C Escherichia coli
4.1.2.21 0.57
-
 D-erythrose 4-phosphate wild type enzyme, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.4 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate Escherichia coli
-
 3-dehydroquinate + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli
-
-
-
4.1.2.21 Escherichia coli
-
-
-
4.2.3.4 Escherichia coli P07639
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.21 glutathione-Sepharose 4B resin column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.4 0.009
-
 strain NR7, plasmid pKL4.79, activity is measured for cells removed from the fermentor 24 h after inoculation Escherichia coli
4.2.3.4 0.01
-
 strain NR7, plasmid pKL4.79, activity is measured for cells removed from the fermentor 36 h after inoculation Escherichia coli
4.2.3.4 0.011
-
 strain NR7, plasmid pKL4.79, activity is measured for cells removed from the fermentor 48 h after inoculation Escherichia coli
4.2.3.4 0.015
-
 strain NR7, plasmid pNR8.075, activity is measured for cells removed from the fermentor 24 h after inoculation Escherichia coli
4.2.3.4 0.016
-
 strain NR7, plasmid pNR8.075, activity is measured for cells removed from the fermentor 36 h after inoculation Escherichia coli
4.2.3.4 0.016
-
 strain NR7, plasmid pNR8.075, activity is measured for cells removed from the fermentor 48 h after inoculation Escherichia coli
4.2.3.4 0.016
-
 strain NR7, plasmid pNR8.288, activity is measured for cells removed from the fermentor 48 h after inoculation Escherichia coli
4.2.3.4 0.017
-
 strain NR7, plasmid pNR8.288, activity is measured for cells removed from the fermentor 24 h after inoculation Escherichia coli
4.2.3.4 0.017
-
 strain NR7, plasmid pNR8.288, activity is measured for cells removed from the fermentor 36 h after inoculation Escherichia coli
4.2.3.4 0.022
-
 strain NR7, plasmid pNR9.127, activity is measured for cells removed from the fermentor 36 h after inoculation Escherichia coli
4.2.3.4 0.023
-
 strain NR7, plasmid pNR8.294, activity is measured for cells removed from the fermentor 48 h after inoculation Escherichia coli
4.2.3.4 0.024
-
 strain NR7, plasmid pNR9.127, activity is measured for cells removed from the fermentor 48 h after inoculation Escherichia coli
4.2.3.4 0.027
-
 strain NR7, plasmid pNR9.127, activity is measured for cells removed from the fermentor 24 h after inoculation Escherichia coli
4.2.3.4 0.039
-
 strain NR7, plasmid pNR8.294, activity is measured for cells removed from the fermentor 36 h after inoculation Escherichia coli
4.2.3.4 0.043
-
 strain NR7, plasmid pNR8.294, activity is measured for cells removed from the fermentor 24 h after inoculation Escherichia coli
4.2.3.4 0.53
-
 strain NR7, plasmid pNR9.056, activity is measured for cells removed from the fermentor 24 h after inoculation Escherichia coli
4.2.3.4 0.92
-
 strain NR7, plasmid pNR9.056, activity is measured for cells removed from the fermentor 36 h after inoculation Escherichia coli
4.2.3.4 1.17
-
 strain NR7, plasmid pNR9.056, activity is measured for cells removed from the fermentor 48 h after inoculation Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
4.1.2.21 2-dehydro-3-deoxy-D-galactonate 6-phosphate
-
 Escherichia coli pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.21 pyruvate + D-erythrose 4-phosphate
-
 Escherichia coli 3-deoxy-D-arabino-heptulosonic acid 7-phosphate
-
 r
4.2.3.4 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate
-
 Escherichia coli 3-dehydroquinate + phosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase
-
 Escherichia coli
2.5.1.54 DAHP synthase
-
 Escherichia coli
4.1.2.21 2-keto-3-deoxy-6-phosphogalactonate aldolase
-
 Escherichia coli
4.1.2.21 DgoA
-
 Escherichia coli
4.1.2.21 KDPGal aldolase
-
 Escherichia coli
4.2.3.4 3-dehydroquinate synthase
-
 Escherichia coli
4.2.3.4 DHQ synthase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.21 0.94
-
 D-erythrose 4-phosphate wild type enzyme, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C Escherichia coli
4.1.2.21 2.5
-
 D-erythrose 4-phosphate mutant enzyme F33I/D58N/Q72H/A75V/V85A/V154F, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.4 7.5
-
 activity assay Escherichia coli