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Literature summary extracted from

  • Wu, T.K.; Liu, Y.T.; Chang, C.H.; Yu, M.T.; Wang, H.J.
    Site-saturated mutagenesis of histidine 234 of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase demonstrates dual functions in cyclization and rearrangement reactions (2006), J. Am. Chem. Soc., 128, 6414-6419.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.7 H234A products are 17% protosta-12,24-dien-3beta-ol, 13% protosta-20,24-dien-3beta-ol, 30% lanosterol, 40% parkeol Saccharomyces cerevisiae
5.4.99.7 H234C products are 7% protosta-12,24-dien-3beta-ol, 4% protosta-20,24-dien-3beta-ol, 67% lanosterol, 22% parkeol Saccharomyces cerevisiae
5.4.99.7 H234D products are 58% iso-malabarica-14(16),17,21-trien-3beta-ol, 30% lanosterol, 12% parkeol Saccharomyces cerevisiae
5.4.99.7 H234E products are 49% lanosterol, 51% parkeol Saccharomyces cerevisiae
5.4.99.7 H234F products are 66% achilleol A, 14% lanosterol, 20% parkeol Saccharomyces cerevisiae
5.4.99.7 H234G products are 29% protosta-12,24-dien-3beta-ol, 7% protosta-20,24-dien-3beta-ol, 17% lanosterol, 47% parkeol Saccharomyces cerevisiae
5.4.99.7 H234I products are 70% lanosterol, 30% parkeol Saccharomyces cerevisiae
5.4.99.7 H234K no catalytic activity Saccharomyces cerevisiae
5.4.99.7 H234L products are 39% lanosterol, 31% parkeol, 30% iso-malabarica-14(16),17,21-trien-3beta-ol Saccharomyces cerevisiae
5.4.99.7 H234M products are 17% achilleol A, 10% iso-malabarica-14(16),17,21-trien-3beta-ol, 30% lanosterol, 40% parkeol Saccharomyces cerevisiae
5.4.99.7 H234N products are 23% protosta-12,24-dien-3beta-ol, 14% protosta-20,24-dien-3beta-ol, 27% lanosterol, 10% parkeol, 26% iso-malabarica-14(16),17,21-trien-3beta-ol Saccharomyces cerevisiae
5.4.99.7 H234P products are 64% lanosterol, 36% parkeol Saccharomyces cerevisiae
5.4.99.7 H234Q product is 100% lanosterol, like wild-type Saccharomyces cerevisiae
5.4.99.7 H234R no catalytic activity Saccharomyces cerevisiae
5.4.99.7 H234S product is 100% protosta-12,24-dien-3beta-ol Saccharomyces cerevisiae
5.4.99.7 H234T product is 100% parkeol Saccharomyces cerevisiae
5.4.99.7 H234V products are 42% lanosterol, 58% parkeol Saccharomyces cerevisiae
5.4.99.7 H234W product is 100% parkeol Saccharomyces cerevisiae
5.4.99.7 H234Y products are 14% achilleol A, 26% protosta-12,24-dien-3beta-ol, 51% lanosterol, 9% parkeol Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.7 Saccharomyces cerevisiae
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene = lanosterol mechanism includes formation of a chair-boat 6-6-5 tricyclic Markovnikov cation and protosteryl cation Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.7 (S)-2,3-epoxysqualene
-
 Saccharomyces cerevisiae lanosterol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.7 ERG7
-
 Saccharomyces cerevisiae