EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.8.2 | in complex with inhibitor O,O-dicyclopentylphosphoroamidate. Phosphoryl oxygen of inhibitor is directly coordinated to the catalytic calcium ion | Loligo vulgaris |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.8.1 | D229N/N120D | site-directed mutagenesis, the mutation reorientates the electrostatic environment around the catalytic calcium, inactive mutant | Loligo vulgaris |
3.1.8.1 | D229N/N175D | site-directed mutagenesis, the mutation reorientates the electrostatic environment around the catalytic calcium, inactive mutant | Loligo vulgaris |
3.1.8.1 | E21Q/N120D | site-directed mutagenesis, inactive mutant | Loligo vulgaris |
3.1.8.1 | E21Q/N175D | site-directed mutagenesis, inactive mutant | Loligo vulgaris |
3.1.8.2 | D229N/N120D | catalytically inactive, no change in the calcium coordinating environment | Loligo vulgaris |
3.1.8.2 | D229N/N175D | catalytically inactive, no change in the calcium coordinating environment | Loligo vulgaris |
3.1.8.2 | E21Q/N120D | catalytically inactive | Loligo vulgaris |
3.1.8.2 | E21Q/N175D | catalytically inactive | Loligo vulgaris |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.8.1 | O,O-dicyclopentylphosphoroamidate | substrate analogue, competitive inhibition, binding structure, direct coordination of the phosphoryl oxygen by the active site catalytic calcium ion, overview | Loligo vulgaris | |
3.1.8.2 | O,O-dicyclopentylphosphoroamidate | crystallization data, phosphoryl oxygen of inhibitor is directly coordinated to the catalytic calcium ion | Loligo vulgaris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.8.2 | 2.72 | - |
diisopropyl fluorophosphate | wild-type, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris | |
3.1.8.2 | 23.36 | - |
diisopropyl fluorophosphate | wild-type, presence of 1 mM O,O-dicyclopentylphosphoroamidate, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris | |
3.1.8.2 | 65.45 | - |
diisopropyl fluorophosphate | wild-type, presence of 3 mM O,O-dicyclopentylphosphoroamidate, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.8.1 | Ca2+ | required for catalysis, bound at the active site, coordinating residues are E21 and D229 | Loligo vulgaris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.8.1 | Loligo vulgaris | - |
- |
- |
3.1.8.2 | Loligo vulgaris | Q7SIG4 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.8.1 | an aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol | catalytic mechanism | Loligo vulgaris | |
3.1.8.2 | diisopropyl fluorophosphate + H2O = diisopropyl phosphate + fluoride | calcium coordinating residue D229, in concert with direct substrate activation by the metal ion, renders the phosphorus atom of the substrate susceptible for attack of water, through generation of a phosphoenzyme intermediate | Loligo vulgaris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.8.1 | sarin + H2O | a highly toxic organophosphorus nerve agent | Loligo vulgaris | methylphosphonofluoride acid + ? | - |
? | |
3.1.8.1 | soman + H2O | i.e. pinacolyl methylphosphonofluoridate, a highly toxic organophosphorus nerve agent | Loligo vulgaris | methylphosphonofluoride acid + 3,3-dimethylbutan-2-ol | - |
? | |
3.1.8.1 | tabun + H2O | a highly toxic organophosphorus nerve agent | Loligo vulgaris | ? | - |
? | |
3.1.8.2 | diisopropyl fluorophosphate + H2O | - |
Loligo vulgaris | diisopropyl phosphate + fluoride | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.8.1 | DFPase | - |
Loligo vulgaris |
3.1.8.1 | diisopropyl fluorophosphatase | - |
Loligo vulgaris |
3.1.8.1 | phosphotriesterase | - |
Loligo vulgaris |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.8.2 | 2091 | - |
diisopropyl fluorophosphate | wild-type, presence of 3 mM O,O-dicyclopentylphosphoroamidate, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris | |
3.1.8.2 | 2107 | - |
diisopropyl fluorophosphate | wild-type, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris | |
3.1.8.2 | 2111 | - |
diisopropyl fluorophosphate | wild-type, presence of 1 mM O,O-dicyclopentylphosphoroamidate, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.8.2 | 0.125 | - |
O,O-dicyclopentylphosphoroamidate | wild-type, pH 7.5, 25°C, in nitrogen atmosphere | Loligo vulgaris |