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Literature summary extracted from

  • Iwig, D.F.; Uchida, A.; Stromberg, J.A.; Booker, S.J.
    The activity of Escherichia coli cyclopropane fatty acid synthase depends on the presence of bicarbonate (2005), J. Am. Chem. Soc., 127, 11612-11613.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.79 bicarbonate required. CFA synthase isolated and assayed in potassium bicarbonate buffer displayes more than 3-fold higher activity than in HEPES buffer Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.79 E239A mutant shows 0.57% of wild-type activity Escherichia coli
2.1.1.79 E239D mutant shows 0.96% of wild-type activity Escherichia coli
2.1.1.79 H266A mutant shows 2.1% of wild-type activity Escherichia coli
2.1.1.79 Y317F mutant shows 0.45% of wild-type activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.79 Borate competitive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.79 0.0705
-
S-adenosyl-L-methionine purification buffer/assay buffer: KHCO3/HEPES Escherichia coli
2.1.1.79 0.0716
-
S-adenosyl-L-methionine purification buffer/assay buffer: HEPES/KHCO3 Escherichia coli
2.1.1.79 0.0887
-
S-adenosyl-L-methionine purification buffer/assay buffer: HEPES/HEPES Escherichia coli
2.1.1.79 0.0997
-
S-adenosyl-L-methionine purification buffer/assay buffer: KHCO3/KHCO3 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.79 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.79 S-adenosyl-L-methionine + phospholipid olefinic fatty acid
-
Escherichia coli S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
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Synonyms

EC Number Synonyms Comment Organism
2.1.1.79 CFA synthase
-
Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.79 2.02
-
Borate
-
Escherichia coli