EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.79 | bicarbonate | required. CFA synthase isolated and assayed in potassium bicarbonate buffer displayes more than 3-fold higher activity than in HEPES buffer | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.79 | E239A | mutant shows 0.57% of wild-type activity | Escherichia coli |
2.1.1.79 | E239D | mutant shows 0.96% of wild-type activity | Escherichia coli |
2.1.1.79 | H266A | mutant shows 2.1% of wild-type activity | Escherichia coli |
2.1.1.79 | Y317F | mutant shows 0.45% of wild-type activity | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.79 | Borate | competitive | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.79 | 0.0705 | - |
S-adenosyl-L-methionine | purification buffer/assay buffer: KHCO3/HEPES | Escherichia coli | |
2.1.1.79 | 0.0716 | - |
S-adenosyl-L-methionine | purification buffer/assay buffer: HEPES/KHCO3 | Escherichia coli | |
2.1.1.79 | 0.0887 | - |
S-adenosyl-L-methionine | purification buffer/assay buffer: HEPES/HEPES | Escherichia coli | |
2.1.1.79 | 0.0997 | - |
S-adenosyl-L-methionine | purification buffer/assay buffer: KHCO3/KHCO3 | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.79 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.79 | S-adenosyl-L-methionine + phospholipid olefinic fatty acid | - |
Escherichia coli | S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.79 | CFA synthase | - |
Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.79 | 2.02 | - |
Borate | - |
Escherichia coli |