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Literature summary extracted from

  • Cortes, L.; Carvalho, A.L.; Todo-Bom, A.; Faro, C.; Pires, E.; Verissimo, P.
    Purification of a novel aminopeptidase from the pollen of Parietaria judaica that alters epithelial integrity and degrades neuropeptides (2006), J. Allergy Clin. Immunol., 118, 878-884.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.22 1,10-phenanthroline 3% residual activity at 10 mM Parietaria judaica
3.4.11.22 amastatin 1.5% residual activity at 0.01 mM Parietaria judaica
3.4.11.22 bestatin 2% residual activity at 0.01 mM Parietaria judaica
3.4.11.22 Ca2+ 76% residual activity at 1 mM Parietaria judaica
3.4.11.22 chymostatin 39% residual activity at 0.1 mM Parietaria judaica
3.4.11.22 EDTA 1.5% residual activity at 5 mM Parietaria judaica
3.4.11.22 Mg2+ 71% residual activity at 1 mM Parietaria judaica
3.4.11.22 Mn2+ complete inhibition at 1 mM Parietaria judaica
3.4.11.22 additional information not inhibited by pepstatin, L-trans-epoxysuccinyl-leucylamide-(4-guanidino)-butane, alpha1-antitrypsin inhibitor or aprotinin Parietaria judaica
3.4.11.22 N-p-tosyl-L-lysine chloromethyl ketone 64% residual activity at 0.1 mM Parietaria judaica
3.4.11.22 N-p-tosyl-L-phenylalanine chloromethyl ketone 8% residual activity at 0.1 mM Parietaria judaica
3.4.11.22 Phenylmethylsulfonylfluoride 6% residual activity at 1 mM Parietaria judaica
3.4.11.22 Zn2+ 2% residual activity at 1 mM Parietaria judaica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.22 0.4
-
 L-Ala-p-nitroanilide in 50 mM Tris-HCl, pH 8.1, at 30°C Parietaria judaica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.22 98000
-
 SDS-PAGE Parietaria judaica

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.22 Parietaria judaica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.22 DEAE-Sephacel column chromatography, ammonium sulfate precipitation, Superdex 200 gel filtration, HiTrap Q column chromatography, and arginine-Sepharose column chromatography Parietaria judaica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.22 pollen
-
 Parietaria judaica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.22 0.16
-
 pollen extract, in 50 mM Tris-HCl, pH 8.1, at 30°C Parietaria judaica
3.4.11.22 4.88
-
 after 30.5fold purification, in 50 mM Tris-HCl, pH 8.1, at 30°C Parietaria judaica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.22 DRVYIHPFHL + H2O angiotensin I Parietaria judaica L-Asp + L-Arg + VYIHPFHL
-
 ?
3.4.11.22 E-cadherin + H2O
-
 Parietaria judaica ?
-
 ?
3.4.11.22 HSDAVFTDNYTRLRKQMAVKKYLNSILN + H2O protein VIP Parietaria judaica His + SDAVFTDNYTRLRKQM + AVKKYLN + SILN
-
 ?
3.4.11.22 L-Ala-p-nitroanilide + H2O preferred substrate Parietaria judaica L-Ala + p-nitroaniline
-
 ?
3.4.11.22 L-Arg-p-nitroanilide + H2O
-
 Parietaria judaica L-Arg + p-nitroaniline
-
 ?
3.4.11.22 additional information L-Val-p-nitroanilide, L-Leu-p-nitroanilide, L-Ala-Ala-Ala-p-nitroanilide, N-succinyl-Ala-Ala-Val-p-nitroanilide, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, benzoyl-Phe-Val-Arg-p-nitroanilide, and benzoyl-Arg-p-nitroanilide are no substrates Parietaria judaica ?
-
 ?
3.4.11.22 occludin + H2O
-
 Parietaria judaica ?
-
 ?
3.4.11.22 RPKPQQFFGLM + H2O substance P Parietaria judaica RPKPQ + QFF + GLM
-
 ?
3.4.11.22 RPPGFSPFR + H2O bradykinin Parietaria judaica Arg + PPGF + SPFR
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.22 Pj-peptidase
-
 Parietaria judaica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.22 40
-
-
 Parietaria judaica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.22 25 60 40% activity at 25°C, no activity above 60°C Parietaria judaica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.22 9
-
-
 Parietaria judaica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.22 7.5 9
-
 Parietaria judaica