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Literature summary extracted from

  • Latha, G.M.; Srinivas, P.; Muralikrishna, G.
    Purification and characterization of ferulic acid esterase from malted finger millet (Eleusine coracana, Indaf-15) (2007), J. Agric. Food Chem., 55, 9704-9712.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.73 oxalic acid 5 mM, 1.4fold activation Eleusine coracana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 Fe3+ 5 mM, complete inactivation Eleusine coracana
3.1.1.73 iodoacetamide 50 mM, 45% inhibition Eleusine coracana
3.1.1.73 p-chloromercuric benzoate 50 mM, 67% inhibition Eleusine coracana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.000053
-
 p-nitrophenylferulate
-
 Eleusine coracana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.73 Co2+ 5 mM, 1.4fold activation Eleusine coracana
3.1.1.73 Cu+ 5 mM, 2fold activation Eleusine coracana
3.1.1.73 Cu2+ 5 mM, 2.17fold activation Eleusine coracana
3.1.1.73 Ni2+ 5 mM, 1.4fold activation Eleusine coracana
3.1.1.73 Zn2+ 5 mM, 1.4fold activation Eleusine coracana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 16500
-
 gel filtration Eleusine coracana

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Eleusine coracana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Eleusine coracana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 26.8
-
-
 Eleusine coracana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 additional information ferulic acid is enzymatically released from water-soluble polysaccharides and synthetic substrates Eleusine coracana ?
-
 ?
3.1.1.73 p-nitrophenylferulate + H2O
-
 Eleusine coracana ferulate + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer 1 * 16500, SDS-PAGE Eleusine coracana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 45
-
-
 Eleusine coracana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 30 60 30°C: about 60% of maximal activity, 60°C: about 65% of maximal activity Eleusine coracana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 30
-
 15 min, stable Eleusine coracana
3.1.1.73 40
-
 15 min, 20% loss of activity Eleusine coracana
3.1.1.73 50
-
 15 min, about 70% of maximal activity Eleusine coracana
3.1.1.73 60
-
 15 min, 50% loss of activity Eleusine coracana
3.1.1.73 70
-
 15 min, 80% loss of activity Eleusine coracana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6
-
-
 Eleusine coracana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 5.5 8 pH 5.5: about 70% of maximal activity, pH 8.0: about 50% of maximal activity Eleusine coracana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 5.5 9 2 h, 10-20% loss of activity Eleusine coracana