Literature summary extracted from

Kouassi, G.K.; Anantheswaran, R.C.; Knabel, S.J.; Floros, J.D.
Effect of high-pressure processing on activity and structure of alkaline phosphatase and lactate dehydrogenase in buffer and milk (2007), J. Agric. Food Chem., 55, 9520-9529.

General Stability

EC Number	General Stability	Organism
3.1.3.1	activity of alkaline phosphatase is not affected after 6 min of pressure treatments (206-620 MPa), regardless of the medium in which the enzyme is prepared. Increasing the processing time to 12 min significantly reduces the activity of alkaline phosphatase at 620 MPa. Circular dichroism data demonstrated a partial change in the secondary structure of ALP at 620 MPa leading to a random coil structure	Bos taurus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.27	additional information	loss of LDH activity with increasing pressure and time treatment due to the combined effects of denaturation and aggregation, overview	Bos taurus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.1.1.27	extracellular	-	Bos taurus	-	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.27	(S)-lactate + NAD+	Bos taurus	-	pyruvate + NADH + H+	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.27	Bos taurus	-	-	-
3.1.3.1	Bos taurus	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.27	milk	fat-free milk, or 2% fat milk	Bos taurus	-
3.1.3.1	intestinal mucosa	-	Bos taurus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.27	(S)-lactate + NAD+	-	Bos taurus	pyruvate + NADH + H+	-	r

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.27	More	analysis of enzyme structure under high pressure conditions: even the lowest high pressure processing treatment of 206 MPa induces a reduction in LDH activity, and the course of reduction increases with high pressure processing treatment until complete inactivation at 482, 515, and 620 MPa. The structure of LDH shows gradual denaturation after exposure at 206 MPa for 6 min, leading to a random coil structure at both 515 and 620 MPa. The loss of LDH activity with increasing pressure and time treatment was due to the combined effects of denaturation and aggregation, structure analysis by far-ultraviolet circular dichroism spectroscopy and dynamic light scattetering	Bos taurus

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.27	LDH	-	Bos taurus
3.1.3.1	alkaline phosphatase VI-1	-	Bos taurus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.1.27	25	-	assay at	Bos taurus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.27	9.8	-	assay at	Bos taurus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.27	beta-NAD+	-	Bos taurus
1.1.1.27	beta-NADH	-	Bos taurus

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Release 2023.1 (January 2023)