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Literature summary extracted from

  • Falkoski, D.L.; Guimaraes, V.M.; Callegari, C.M.; Reis, A.P.; de Barros, E.G.; de Rezende, S.T.
    Processing of soybean products by semipurified plant and microbial alpha-galactosidases (2006), J. Agric. Food Chem., 54, 10184-10190.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.22 2-mercaptoethanol 107.8% activity at 10 mM Aspergillus terreus
3.2.1.22 D-glucose 105.1% activity at 10 mM Aspergillus terreus
3.2.1.22 D-sucrose 116.5% activity at 10 mM Aspergillus terreus
3.2.1.22 EDTA 105% activity at 10 mM Aspergillus terreus
3.2.1.22 iodoacetamide 104.3% activity at 10 mM Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 2-mercaptoethanol 98.66% activity at 10 mM Glycine max
3.2.1.22 2-mercaptoethanol 95% activity at 10 mM Penicillium griseoroseum
3.2.1.22 Ag+ complete inhibition at 10 mM Aspergillus terreus
3.2.1.22 Ag+ complete inhibition at 10 mM Glycine max
3.2.1.22 Ag+ complete inhibition at 10 mM Penicillium griseoroseum
3.2.1.22 Ca2+ 96.25% activity at 10 mM Glycine max
3.2.1.22 Ca2+ 94% activity at 10 mM Penicillium griseoroseum
3.2.1.22 Cu2+ complete inhibition at 10 mM Aspergillus terreus
3.2.1.22 Cu2+ 59.16% activity at 10 mM Glycine max
3.2.1.22 Cu2+ 11% activity at 10 mM Penicillium griseoroseum
3.2.1.22 D-galactose inhibitory effect at 10 mM Glycine max
3.2.1.22 D-glucose 94.4% activity at 10 mM Glycine max
3.2.1.22 D-glucose 96% activity at 10 mM Penicillium griseoroseum
3.2.1.22 D-lactose 89.51% activity at 10 mM Glycine max
3.2.1.22 D-lactose 88% activity at 10 mM Penicillium griseoroseum
3.2.1.22 D-maltose 92.8% activity at 10 mM Aspergillus terreus
3.2.1.22 D-maltose 91.33% activity at 10 mM Glycine max
3.2.1.22 D-maltose 94% activity at 10 mM Penicillium griseoroseum
3.2.1.22 D-mannose 90.5% activity at 10 mM Aspergillus terreus
3.2.1.22 D-mannose 90.05% activity at 10 mM Glycine max
3.2.1.22 D-mannose 94% activity at 10 mM Penicillium griseoroseum
3.2.1.22 D-sucrose 98.72% activity at 10 mM Glycine max
3.2.1.22 EDTA 97.21% activity at 10 mM Glycine max
3.2.1.22 Hg2+ complete inhibition at 10 mM Aspergillus terreus
3.2.1.22 Hg2+ complete inhibition at 10 mM Glycine max
3.2.1.22 Hg2+ complete inhibition at 10 mM Penicillium griseoroseum
3.2.1.22 iodoacetamide 95.88% activity at 10 mM Glycine max
3.2.1.22 iodoacetamide 98.87% activity at 10 mM Penicillium griseoroseum
3.2.1.22 K+ 96.48% activity at 10 mM Glycine max
3.2.1.22 melibiose 39.7% activity at 10 mM Aspergillus terreus
3.2.1.22 melibiose 74.14% activity at 10 mM Glycine max
3.2.1.22 melibiose 58% activity at 10 mM Penicillium griseoroseum
3.2.1.22 Mg2+ 94.15% activity at 10 mM Glycine max
3.2.1.22 Mg2+ 98% activity at 10 mM Penicillium griseoroseum
3.2.1.22 additional information not inhibited by D-lactose Aspergillus terreus
3.2.1.22 additional information not inhibited by sucrose, EDTA, and K+ Penicillium griseoroseum
3.2.1.22 Na+ 94.2% activity at 10 mM Glycine max
3.2.1.22 Na+ 96% activity at 10 mM Penicillium griseoroseum
3.2.1.22 raffinose 80.60% activity at 10 mM Aspergillus terreus
3.2.1.22 raffinose 81.73% activity at 10 mM Glycine max
3.2.1.22 raffinose 93% activity at 10 mM Penicillium griseoroseum
3.2.1.22 SDS 46.9% activity at 10 mM Aspergillus terreus
3.2.1.22 SDS 49.36% activity at 10 mM Glycine max
3.2.1.22 SDS complete inhibition at 10 mM Penicillium griseoroseum
3.2.1.22 stachyose 95.73% activity at 10 mM Aspergillus terreus
3.2.1.22 stachyose 87.69% activity at 10 mM Glycine max
3.2.1.22 stachyose 88% activity at 10 mM Penicillium griseoroseum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 Ca2+ 109.9% activity at 10 mM Aspergillus terreus
3.2.1.22 K+ 105.7% activity at 10 mM Aspergillus terreus
3.2.1.22 Mg2+ 105.6% activity at 10 mM Aspergillus terreus
3.2.1.22 Na+ 124.8% activity at 10 mM Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Aspergillus terreus
-
-
-
3.2.1.22 Glycine max
-
 cultivar Monarca
-
3.2.1.22 Penicillium griseoroseum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 ammonium sulfate fractionation, Sephacryl S-200 gel filtration Penicillium griseoroseum
3.2.1.22 ammonium sulfate fractionation, Sephadex G-100 gel filtration, CM-Sepharose Fast Flow column chromatography Glycine max
3.2.1.22 Sephacryl S-200 gel filtration Aspergillus terreus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 seed
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 2.02
-
 crude extract, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C Penicillium griseoroseum
3.2.1.22 3.33
-
 crude extract, in 0.1 M sodium acetate buffer, pH 4.0, at 40°C Aspergillus terreus
3.2.1.22 30
-
 crude extract, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C Glycine max
3.2.1.22 55
-
 after 27.2fold purification, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C Penicillium griseoroseum
3.2.1.22 67.3
-
 after 20.20fold purification, in 0.1 M sodium acetate buffer, pH 4.0, at 40°C Aspergillus terreus
3.2.1.22 540
-
 after 19.74fold purification, in 0.1 M sodium acetate buffer, pH 5.0, at 40°C Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Penicillium griseoroseum 4-nitrophenol + D-glucose
-
 ?
3.2.1.22 4-nitrophenyl alpha-D-xylopyranoside + H2O 9.64% activity relative to p-nitrophenyl alpha-D-galactopyranoside Aspergillus terreus 4-nitrophenol + alpha-D-xylopyranose
-
 ?
3.2.1.22 D-sucrose + H2O 16.30% activity relative to p-nitrophenyl alpha-D-galactopyranoside Penicillium griseoroseum D-glucose + D-fructose
-
 ?
3.2.1.22 D-sucrose + H2O 35.71% activity relative to p-nitrophenyl alpha-D-galactopyranoside Aspergillus terreus D-glucose + D-fructose
-
 ?
3.2.1.22 lactose + H2O 2.3% activity relative to p-nitrophenyl alpha-D-galactopyranoside Glycine max beta-D-galactose + D-glucose
-
 ?
3.2.1.22 maltose + H2O 10.60% activity relative to p-nitrophenyl alpha-D-galactopyranoside Penicillium griseoroseum alpha-D-glucose + D-glucose
-
 ?
3.2.1.22 maltose + H2O 18.12% activity relative to p-nitrophenyl alpha-D-galactopyranoside Glycine max alpha-D-glucose + D-glucose
-
 ?
3.2.1.22 maltose + H2O 6.07% activity relative to p-nitrophenyl alpha-D-galactopyranoside Aspergillus terreus alpha-D-glucose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O 15.36% activity relative to p-nitrophenyl alpha-D-galactopyranoside Aspergillus terreus D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O 16.15% activity relative to p-nitrophenyl alpha-D-galactopyranoside Penicillium griseoroseum D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O 7.68% activity relative to p-nitrophenyl alpha-D-galactopyranoside Glycine max D-galactose + D-glucose
-
 ?
3.2.1.22 additional information D-lactose, o-nitrophenyl beta-D-galactopyranoside, p-nitrophenyl alpha-D-glucopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates Aspergillus terreus ?
-
 ?
3.2.1.22 additional information D-sucrose, p-nitrophenyl alpha-D-glucopyranoside, p-nitrophenyl alpha-D-xylulopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates Glycine max ?
-
 ?
3.2.1.22 additional information lactose, o-nitrophenyl beta-D-galactopyranoside, p-nitrophenyl alpha-D-xylulopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates Penicillium griseoroseum ?
-
 ?
3.2.1.22 o-nitrophenyl beta-D-galactopyranoside 4.53% activity relative to p-nitrophenyl alpha-D-galactopyranoside Glycine max o-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O 100% activity Glycine max p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O 100% activity Aspergillus terreus p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O 100% activity Penicillium griseoroseum p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O 30.60% activity relative to p-nitrophenyl alpha-D-galactopyranoside Glycine max sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O 60.65% activity relative to p-nitrophenyl alpha-D-galactopyranoside Penicillium griseoroseum sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O 78.57% activity relative to p-nitrophenyl alpha-D-galactopyranoside Aspergillus terreus sucrose + D-galactose
-
 ?
3.2.1.22 stachyose + 2 H2O 11.74% activity relative to p-nitrophenyl alpha-D-galactopyranoside Glycine max 2 alpha-D-galactose + sucrose
-
 ?
3.2.1.22 stachyose + 2 H2O 78.65% activity relative to p-nitrophenyl alpha-D-galactopyranoside Penicillium griseoroseum 2 alpha-D-galactose + sucrose
-
 ?
3.2.1.22 stachyose + 2 H2O 85.71% activity relative to p-nitrophenyl alpha-D-galactopyranoside Aspergillus terreus 2 alpha-D-galactose + sucrose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 Alpha-D-galactoside galactohydrolase
-
 Glycine max
3.2.1.22 Alpha-D-galactoside galactohydrolase
-
 Aspergillus terreus
3.2.1.22 Alpha-D-galactoside galactohydrolase
-
 Penicillium griseoroseum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 45
-
-
 Penicillium griseoroseum
3.2.1.22 50
-
-
 Glycine max
3.2.1.22 65
-
-
 Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 40 50 no loss in enzyme activity is observed after incubation for 5 h at 40°C, the enzyme maintains about 65% of its original activity after 2 h at 45°C, about 90% of the activity is lost during 30 min at 50°C Penicillium griseoroseum
3.2.1.22 40 50 the enzyme maintains about 90% and 70% of its original activity after 4 h and 10 h at 40°C, respectively, at 50°C 91% of its original activity is lost after 30 min of incubation Glycine max
3.2.1.22 60
-
 the enzyme maintains about 100% and 40% of its original activity after 3 h and 36 h at 60°C, respectively Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 4
-
-
 Aspergillus terreus
3.2.1.22 5
-
-
 Glycine max
3.2.1.22 5
-
-
 Penicillium griseoroseum