EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.63 | additional information | stabilization of the enzyme in entrapped Ca-alginate beads, activity maximal (ca. 48%) at 2% (w/v) sodium alginate concentration | Aspergillus oryzae |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.21.63 | industry | immobilization of the enzyme as an important biotechnological aspect | Aspergillus oryzae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.63 | Hg2+ | strongly inhibits | Aspergillus oryzae | |
3.4.21.63 | Mn2+ | slightly inhibits | Aspergillus oryzae | |
3.4.21.63 | additional information | not inhibited by Cu2+, Sn2+, Ba2+, Co2+, Li+, benzamidine, beta-ME, cysteine, EDTA and 1,10-phenathroline | Aspergillus oryzae | |
3.4.21.63 | NaN3 | slightly inhibits | Aspergillus oryzae | |
3.4.21.63 | phenylmethylsulfonyl fluoride | completely inhibits | Aspergillus oryzae | |
3.4.21.63 | TLCK | moderately inhibits | Aspergillus oryzae | |
3.4.21.63 | TPCK | moderately inhibits | Aspergillus oryzae | |
3.4.21.63 | Trypsin inhibitor | moderately inhibits | Aspergillus oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.63 | additional information | - |
additional information | Km for the immobilized enzyme is 1.5fold higher than for the free enzyme | Aspergillus oryzae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.63 | Ca2+ | activates 35% at 1 mM | Aspergillus oryzae | |
3.4.21.63 | Mg2+ | activates 19% at 1 mM | Aspergillus oryzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.63 | 33000 | - |
gel filtration | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.63 | Aspergillus oryzae | - |
- |
- |
3.4.21.63 | Aspergillus oryzae AWT20 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.63 | partially purified by ion exchange chromatography and gel filtration, 30fold | Aspergillus oryzae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.21.63 | 25.7 | - |
crude extract | Aspergillus oryzae |
3.4.21.63 | 750 | - |
30fold purified enzyme | Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.63 | casein + H2O | active centre of the enzyme contains serine residues | Aspergillus oryzae | hydrolyzed casein | - |
? | |
3.4.21.63 | casein + H2O | active centre of the enzyme contains serine residues | Aspergillus oryzae AWT20 | hydrolyzed casein | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.21.63 | monomer | 1 * 33000, SDS-PAGE | Aspergillus oryzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.63 | Alkaline protease | - |
Aspergillus oryzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.63 | 55 | - |
immobilized enzyme retains 86% of its activity while the free enzyme retains only 10% | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.63 | 9.5 | - |
immobilized enzyme | Aspergillus oryzae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.63 | 5.5 | 10 | immobilized enzyme, shows enhanced stability in acidic as well as alkaline environments in comparison to the free enzyme (stable between pH 7.0-9.0) | Aspergillus oryzae |