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Literature summary extracted from

  • Fan, J.; Yang, W.; Brindley, P.J.
    Lysophospholipase from the human blood fluke, Schistosoma japonicum (2007), Int. J. Infect. Dis., 12, 143-151.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.5 medicine Schistosome lysophospholipase may represent a new target for anti-schistosomal chemotherapy Schistosoma japonicum
3.1.1.5 medicine schistosome lysophospholipase may represent a new target for anti-schistosomal-chemotherapy Schistosoma japonicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.5 expressed in Escherichia coli Schistosoma japonicum
3.1.1.5 expressed in Escherichia coli as a His-tagged fusion protein Schistosoma japonicum
3.1.1.5 into the vector pQE30 for expression in Escherichia coli cells Schistosoma japonicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.5 23000
-
 determined by Western blot analysis, extract of adult Schistosoma japonicum worm Schistosoma japonicum
3.1.1.5 23000
-
 immunoblot analysis, single band in both membrane-associated and soluble tissue fractions of adult schistosomes Schistosoma japonicum
3.1.1.5 24840
-
 calculated Schistosoma japonicum
3.1.1.5 24840
-
 calculated from cDNA Schistosoma japonicum
3.1.1.5 26000
-
 SDS-PAGE, recombinant protein Schistosoma japonicum
3.1.1.5 26000
-
 determined by SDS-PAGE, recombinant 6-His-tagged lysophospholipase Schistosoma japonicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.5 1-acyl-2-lysophosphatidylcholine + H2O Schistosoma japonicum
-
 fatty acid + glycerophosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Schistosoma japonicum
-
-
-
3.1.1.5 Schistosoma japonicum Q9U8F2
-
-
3.1.1.5 Schistosoma japonicum Q9U8F2 enzyme exhibits the GXSXG consensus active site characteristic of serine proteases, esterases, and lipases, and includes the catalytic triad motif of Ser—Asp—His residues characteristic of serine hydrolases. Three-dimensional structural predictions indicate that the putative catalytic triad formed by these three residues is located at the alpha/beta-hydrolase fold characteristic of the lipases and esterases
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.5 by affinity chromatography on Ni-NTA Superflow resin Schistosoma japonicum
3.1.1.5 using Ni-NTA chromatography Schistosoma japonicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.5 adult
-
 Schistosoma japonicum
-
3.1.1.5 egg
-
 Schistosoma japonicum
-
3.1.1.5 egg transcript is isolated from the egg stage Schistosoma japonicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 1-acyl-2-lysophosphatidylcholine + H2O
-
 Schistosoma japonicum fatty acid + glycerophosphocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.5 lysophospholipase
-
 Schistosoma japonicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.5 Schistosoma japonicum determined by isoelectric focusing, SDS-PAGE, extract of adult Schistosoma japonicum worm
-
 6.05
3.1.1.5 Schistosoma japonicum predicted
-
 7.06