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Literature summary extracted from

  • Sathish, H.A.; Kumar, P.R.; Prakash, V.
    Mechanism of solvent induced thermal stabilization of papain (2007), Int. J. Biol. Macromol., 41, 383-390.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.22.2 in the presence of 20% (w/v) sorbitol the enzyme retains nearly 65% of its activity after heating at 75°C for 40 min and it retains almost 45% of its activity after heating for 80 min, the maximum effect is seen in the case of 40% (w/v) sorbitol, where the enzyme retains nearly 80 and 70% of its activity after heating at 75°C for 40 and 80 min, respectively, the enzyme also shows increased thermal stability in the presence of sucrose or xylose Carica papaya

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.22.2 Glycerol in the presence of 20% (v/v) glycerol the enzyme retains nearly 80% of its activity even after heating at 75°C for 40 min and it retains almost 70% of its activity even after heating for 80 min, at 30% (v/v) glycerol the enzyme retains nearly 80 and 65% of its activity after heating for 40 and 80 min, respectively at 75°C Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
-
Carica papaya N-alpha-benzoyl-DL-Arg + 4-nitroaniline
-
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Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.2 75
-
the native enzyme loses nearly 50% of its activity after heating at 75°C for 40 min, and it retains only 20% activity after heating for 80 min Carica papaya