Any feedback?
Literature summary extracted from
- Catechol 1,2-dioxygenase from Pseudomonas putida in organic media - an electron paramagnetic resonance study (2003), Int. J. Biol. Macromol., 33, 101-106.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.1 | Iron | non-heme iron of the enzyme | Pseudomonas putida |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 59000 | - |
isozyme 3, gel filtration | Pseudomonas putida |
| 1.13.11.1 | 64000 | - |
isozyme 2, gel filtration | Pseudomonas putida |
| 1.13.11.1 | 66900 | - |
isozyme 1, gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.1 | catechol + O2 | Pseudomonas putida | - |
cis,cis-muconate | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.13.11.1 | hexane | isozyme 1 is able to function in a non-aqueous environment catalyzing the oxidation of catechol in n-hexane, lyophilized isozyme in n-hexane, overview | Pseudomonas putida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.1 | Pseudomonas putida | - |
strain DSM 437, isozymes 1-3 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.1 | native isozymes 1-3 by ammonium sulfate fractionation, and affinity and anion exchange chromatography | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 0.87 | - |
crude enzyme | Pseudomonas putida |
| 1.13.11.1 | 12 | - |
purified isozyme 3 | Pseudomonas putida |
| 1.13.11.1 | 17.9 | - |
purified isozyme 2 | Pseudomonas putida |
| 1.13.11.1 | 22.4 | - |
purified isozyme 1 | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.1 | catechol + O2 | - |
Pseudomonas putida | cis,cis-muconate | - |
? | |
| 1.13.11.1 | catechol + O2 | isozyme1 is able to function as lyophilized enzyme in a non-aqueous environment catalyzing the oxidation of catechol in n-hexane, overview | Pseudomonas putida | cis,cis-muconate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.1 | pyrocatechase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 25 | - |
assay at, in aqueous medium | Pseudomonas putida |
| 1.13.11.1 | 35 | - |
assay at, in n-hexane | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 7 | - |
assay at, in aqueous medium | Pseudomonas putida |
| 1.13.11.1 | 8.5 | - |
assay at, in n-hexane | Pseudomonas putida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
