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Literature summary extracted from

  • Knizetova, P.; Vancova, I.; Kocakova, P.; Slovak, M.; Proost, P.; Kopacek, J.
    New member of the protein disulfide isomerase (PDI) family identified in Amblyomma variegatum tick (2006), Insect Biochem. Mol. Biol., 36, 943-953.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.4.1 cloning from RNA by 3' race RT-PCR, DNA and amino acid sequence determination and analysis, sequence comparison, expression as GST-tagged or His-tagged enzyme in Escherichia coli strain BL21(DE3) Amblyomma variegatum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.4.1 endoplasmic reticulum PDI contains an endoplasmic reticulum-retention KEEL signal Amblyomma variegatum 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.4.1 55000
-
 x * 55000, SDS-PAGE Amblyomma variegatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.4.1 additional information Amblyomma variegatum PDI has an important function in the correct folding of nascent polypeptides, which is a crucial step in the mechanism which delivers tick proteins to the secretion pathway important for blood feeding ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.4.1 Amblyomma variegatum A0SHR2 a tick cultured on rabbit hosts, constitutive gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.4.1 recombinant GST-tagged or His-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione or nickel affinity chromatography, respectively, native enzyme partially by isoelectric focusing/2D PAGE Amblyomma variegatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.4.1 fibre tract cells of the acini type II and III of salivary gland Amblyomma variegatum
-
5.3.4.1 gonad male tick Amblyomma variegatum
-
5.3.4.1 additional information the PDI expression levels in salivary gland is uniform in unfed ticks and ticks fed for different time intervals, no differences in enzyme expression between female and male ticks, PDI tissue distribution analysis, overview Amblyomma variegatum
-
5.3.4.1 salivary gland
-
 Amblyomma variegatum
-
5.3.4.1 trachea male ticks Amblyomma variegatum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.4.1 insulin + DTT bovine substrate, reductase activity with DTT Amblyomma variegatum ?
-
 ?
5.3.4.1 additional information PDI has an important function in the correct folding of nascent polypeptides, which is a crucial step in the mechanism which delivers tick proteins to the secretion pathway important for blood feeding Amblyomma variegatum ?
-
 ?
5.3.4.1 additional information the enzyme shows dithiol-disulfide-oxidoreductase activity, and contains a conserved WCGHC active site and two thioredoxin domains Amblyomma variegatum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.4.1 ? x * 55000, SDS-PAGE Amblyomma variegatum
5.3.4.1 More domain organization of PDI, overview Amblyomma variegatum

Synonyms

EC Number Synonyms Comment Organism
5.3.4.1 More the enzyme belongs to the family of protein disulfide isomerases Amblyomma variegatum
5.3.4.1 PDI
-
 Amblyomma variegatum
5.3.4.1 protein disulfide isomerase
-
 Amblyomma variegatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.4.1 6.5
-
 assay at Amblyomma variegatum

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.4.1 DTT
-
 Amblyomma variegatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.4.1 Amblyomma variegatum isoelectric focusing
-
 6.64