BRENDA - Enzyme Database

Structural studies and mechanism of Saccharomyces cerevisiae dolichyl-phosphate-mannose synthase: insights into the initial step of synthesis of dolichyl-phosphate-linked oligosaccharide chains in membranes of endoplasmic reticulum

Lamani, E.; Mewbourne, R.B.; Fletcher, D.S.; Maltsev, S.D.; Danilov, L.L.; Veselovsky, V.V.; Lozanova, A.V.; Grigorieva, N.Y.; Pinsker, O.A.; Xing, J.; Forsee, W.T.; Cheung, H.C.; Schutzbach, J.S.; Shibaev, V.N.; Jedrzejas, M.J.; Glycobiology 16, 666-678 (2006)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
additional information
-
additional information
KM-values for synthetic dolichyl phosphate analogs
Saccharomyces cerevisiae
2.4.1.83
0.0246
-
(6Z,10Z,14Z,18Z,22E,26E)-3,7,11,15,19,23,27-heptamethyl-30-(naphthalen-1-ylamino)triaconta-6,10,14,18,22,26-hexaen-1-yl dihydrogen phosphate
pH 7.5
Saccharomyces cerevisiae
2.4.1.83
0.0248
-
(6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-3,7,11,15,19,23,27,31,35,39-decamethyl-42-(naphthalen-1-ylamino)dotetraconta-6,10,14,18,22,26,30,34,38-nonaen-1-yl dihydrogen phosphate
pH 7.5
Saccharomyces cerevisiae
2.4.1.83
0.0751
-
(6Z,10E,14E,18E,22E)-3,7,15,19,23,27-hexamethyl-11-[(naphthalen-1-ylamino)methyl]octacosa-6,10,14,18,22,26-hexaen-1-yl dihydrogen phosphate
pH 7.5
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
endoplasmic reticulum
membrane
Saccharomyces cerevisiae
5783
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.83
GDP-mannose + dolichyl phosphate
Saccharomyces cerevisiae
the enzyme transfers mannose from the water soluble cytoplasmic donor GDP-Man, to the membrane bound, extremely hydrophobic, and long-chain polyisoprenoid acceptor, dolichyl-phosphate
GDP + dolichyl D-mannosyl phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Saccharomyces cerevisiae
-
recombinant
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.83
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
(6Z,10E,14E,18E,22E)-3,7,15,19,23,27-hexamethyl-11-[(naphthalen-1-ylamino)methyl]octacosa-6,10,14,18,22,26-hexaen-1-yl dihydrogen phosphate + dolichyl phosphate
-
680037
Saccharomyces cerevisiae
?
-
-
-
?
2.4.1.83
(6Z,10Z,14Z,18Z,22E,26E)-3,7,11,15,19,23,27-heptamethyl-30-(naphthalen-1-ylamino)triaconta-6,10,14,18,22,26-hexaen-1-yl dihydrogen phosphate + dolichyl phosphate
-
680037
Saccharomyces cerevisiae
?
-
-
-
?
2.4.1.83
(6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-3,7,11,15,19,23,27,31,35,39-decamethyl-42-(naphthalen-1-ylamino)dotetraconta-6,10,14,18,22,26,30,34,38-nonaen-1-yl dihydrogen phosphate + dolichyl phosphate
-
680037
Saccharomyces cerevisiae
?
-
-
-
?
2.4.1.83
GDP-mannose + dolichyl phosphate
the enzyme transfers mannose from the water soluble cytoplasmic donor GDP-Man, to the membrane bound, extremely hydrophobic, and long-chain polyisoprenoid acceptor, dolichyl-phosphate
680037
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
2.4.1.83
GDP-mannose + dolichyl phosphate
or synthetic analogs of dolichyl phosphate
680037
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
additional information
-
additional information
KM-values for synthetic dolichyl phosphate analogs
Saccharomyces cerevisiae
2.4.1.83
0.0246
-
(6Z,10Z,14Z,18Z,22E,26E)-3,7,11,15,19,23,27-heptamethyl-30-(naphthalen-1-ylamino)triaconta-6,10,14,18,22,26-hexaen-1-yl dihydrogen phosphate
pH 7.5
Saccharomyces cerevisiae
2.4.1.83
0.0248
-
(6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-3,7,11,15,19,23,27,31,35,39-decamethyl-42-(naphthalen-1-ylamino)dotetraconta-6,10,14,18,22,26,30,34,38-nonaen-1-yl dihydrogen phosphate
pH 7.5
Saccharomyces cerevisiae
2.4.1.83
0.0751
-
(6Z,10E,14E,18E,22E)-3,7,15,19,23,27-hexamethyl-11-[(naphthalen-1-ylamino)methyl]octacosa-6,10,14,18,22,26-hexaen-1-yl dihydrogen phosphate
pH 7.5
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
endoplasmic reticulum
membrane
Saccharomyces cerevisiae
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.83
GDP-mannose + dolichyl phosphate
Saccharomyces cerevisiae
the enzyme transfers mannose from the water soluble cytoplasmic donor GDP-Man, to the membrane bound, extremely hydrophobic, and long-chain polyisoprenoid acceptor, dolichyl-phosphate
GDP + dolichyl D-mannosyl phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
(6Z,10E,14E,18E,22E)-3,7,15,19,23,27-hexamethyl-11-[(naphthalen-1-ylamino)methyl]octacosa-6,10,14,18,22,26-hexaen-1-yl dihydrogen phosphate + dolichyl phosphate
-
680037
Saccharomyces cerevisiae
?
-
-
-
?
2.4.1.83
(6Z,10Z,14Z,18Z,22E,26E)-3,7,11,15,19,23,27-heptamethyl-30-(naphthalen-1-ylamino)triaconta-6,10,14,18,22,26-hexaen-1-yl dihydrogen phosphate + dolichyl phosphate
-
680037
Saccharomyces cerevisiae
?
-
-
-
?
2.4.1.83
(6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-3,7,11,15,19,23,27,31,35,39-decamethyl-42-(naphthalen-1-ylamino)dotetraconta-6,10,14,18,22,26,30,34,38-nonaen-1-yl dihydrogen phosphate + dolichyl phosphate
-
680037
Saccharomyces cerevisiae
?
-
-
-
?
2.4.1.83
GDP-mannose + dolichyl phosphate
the enzyme transfers mannose from the water soluble cytoplasmic donor GDP-Man, to the membrane bound, extremely hydrophobic, and long-chain polyisoprenoid acceptor, dolichyl-phosphate
680037
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
2.4.1.83
GDP-mannose + dolichyl phosphate
or synthetic analogs of dolichyl phosphate
680037
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
-
-
?