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Literature summary extracted from

  • Rabbani, S.; Miksa, V.; Wipf, B.; Ernst, B.
    Molecular cloning and functional expression of a novel Helicobacter pylori alpha-1,4 fucosyltransferase (2005), Glycobiology, 15, 1076-1083.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.65 synthesis the enzyme possesses a broad tolerance toward nonnatural type I acceptor substrate analogs and therefore represents a valuable tool for the chemoenzymatic synthesis of Lewis A, sialyl Lewis A as well as mimetics thereof Helicobacter pylori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.65 expression in Escherichia coli Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.65 NEM 10 mM, 90% inhibition Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.65 0.00573
-
 GDP-beta-L-fucose 37°C, pH 6.5 Helicobacter pylori
2.4.1.65 0.111
-
 Siaalpha-2,3Galbeta-1,3GlcNAc-R 37°C, pH 6.5 Helicobacter pylori
2.4.1.65 0.114
-
 Galbeta-1,3GlcNAc-R 37°C, pH 6.5 Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.65 50502
-
 x * 50502, SDS-PAGE Helicobacter pylori
2.4.1.65 64500
-
 x * 64500, SDS-PAGE Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.65 Helicobacter pylori Q6ST35
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.65 recombinant Helicobacter pylori

Storage Stability

EC Number Storage Stability Organism
2.4.1.65 -70°C, recombinant enzyme is stable for several months in cacodylate buffer containing Helicobacter pylori
2.4.1.65 10% glycerol. Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.65 GDP-beta-L-fucose + Gal-beta-(1->3)-GlcNAc-beta-O-(CH2)8COOCH3
-
 Helicobacter pylori GDP + Gal-beta-(1->3)-[Fuc-alpha-(1->4)]-GlcNAc-beta-O-(CH2)8COOCH3
-
 ?
2.4.1.65 GDP-beta-L-fucose + Galbeta-1,3GlcNAc-R
-
 Helicobacter pylori ?
-
 ?
2.4.1.65 GDP-beta-L-fucose + Galbeta1,3GlcNAc-O(CH2)8CO2CH3
-
 Helicobacter pylori ?
-
 ?
2.4.1.65 GDP-beta-L-fucose + NeuAcalpha1-3Galbeta1-3GlcNAc-beta-O-(CH2)8COOCH3
-
 Helicobacter pylori ?
-
 ?
2.4.1.65 GDP-beta-L-fucose + Siaalpha-2,3Galbeta-1,3GlcNAc-R
-
 Helicobacter pylori ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.65 ? x * 64500, SDS-PAGE Helicobacter pylori
2.4.1.65 ? x * 50502, SDS-PAGE Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.4.1.65 alpha-1,4 fucosyltransferase
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.65 37 42
-
 Helicobacter pylori

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.65 25 50 25°C: 70% of maximal activity, 50°C: about 60% of maximal activity Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.65 3.13
-
 GDP-beta-L-fucose 37°C, pH 6.5 Helicobacter pylori
2.4.1.65 3.947
-
 GDP-beta-L-fucose 37°C, pH 6.5 Helicobacter pylori
2.4.1.65 8.1
-
 Galbeta-(1-3)-GlcNAc-O-(CH2)8COOCH3 37°C, pH 6.5 Helicobacter pylori
2.4.1.65 26.12
-
 Galbeta-(1-3)-GlcNAc-O-(CH2)8COOCH3 37°C, pH 6.5 Helicobacter pylori
2.4.1.65 34.33
-
 NeuAcalpha1-3Galbeta1-3GlcNAc-beta-O-(CH2)8COOCH3 37°C, pH 6.5 Helicobacter pylori