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Literature summary extracted from

  • Aurilia, V.; Parracino, A.; Saviano, M.; Rossi, M.; DAuria, S.
    The psychrophilic bacterium Pseudoalteromonas haloplanktis TAC125 possesses a gene coding for a cold-adapted feruloyl esterase activity that shares homology with esterase enzymes from gamma-proteobacteria and yeast (2007), Gene, 397, 51-57.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Escherichia coli Pseudoalteromonas haloplanktis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.000025
-
 methylumbelliferyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
3.1.1.73 0.27
-
 4-methylumbelliferyl p-trimethylammonio cinnamate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
3.1.1.73 0.5
-
 Alpha-naphthyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
3.1.1.73 0.5
-
 beta-naphthyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
3.1.1.73 0.75
-
 p-nitrophenyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.73 Ca2+ stimulates Pseudoalteromonas haloplanktis
3.1.1.73 Mg2+ stimulates Pseudoalteromonas haloplanktis
3.1.1.73 Mn2+ stimulates Pseudoalteromonas haloplanktis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Pseudoalteromonas haloplanktis Q3IL66
-
-
3.1.1.73 Pseudoalteromonas haloplanktis TAC 125 Q3IL66
-
-

Storage Stability

EC Number Storage Stability Organism
3.1.1.73 4°C, 3 days, stable Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-methylumbelliferyl p-trimethylammonio cinnamate + H2O
-
 Pseudoalteromonas haloplanktis 4-methylumbelliferone + p-trimethylammonio cinnamate
-
 ?
3.1.1.73 4-methylumbelliferyl p-trimethylammonio cinnamate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 4-methylumbelliferone + p-trimethylammonio cinnamate
-
 ?
3.1.1.73 alpha-naphthyl acetate + H2O
-
 Pseudoalteromonas haloplanktis alpha-naphthol + acetate
-
 ?
3.1.1.73 alpha-naphthyl acetate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 alpha-naphthol + acetate
-
 ?
3.1.1.73 beta-naphthyl acetate + H2O
-
 Pseudoalteromonas haloplanktis beta-naphthol + acetate
-
 ?
3.1.1.73 beta-naphthyl acetate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 beta-naphthol + acetate
-
 ?
3.1.1.73 methylumbelliferyl acetate + H2o
-
 Pseudoalteromonas haloplanktis methylumbelliferone + acetate
-
 ?
3.1.1.73 additional information no activity with p-nitrophenyl esanoate and p-nitrophenyl-dodecanoate Pseudoalteromonas haloplanktis ?
-
 ?
3.1.1.73 additional information no activity with p-nitrophenyl esanoate and p-nitrophenyl-dodecanoate Pseudoalteromonas haloplanktis TAC 125 ?
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Pseudoalteromonas haloplanktis p-nitrophenol + acetate
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 p-nitrophenol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 30000., SDS-PAGE Pseudoalteromonas haloplanktis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 PSHAa enzyme
-
 Pseudoalteromonas haloplanktis
3.1.1.73 PSHAa1385
-
 Pseudoalteromonas haloplanktis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 20
-
-
 Pseudoalteromonas haloplanktis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.1
-
 methylumbelliferyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
3.1.1.73 1.8
-
 beta-naphthyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
3.1.1.73 2
-
 p-nitrophenyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
3.1.1.73 2.75
-
 Alpha-naphthyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
3.1.1.73 11.9
-
 4-methylumbelliferyl p-trimethylammonio cinnamate 20°C, pH 7.4 Pseudoalteromonas haloplanktis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 8
-
-
 Pseudoalteromonas haloplanktis