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Literature summary extracted from

  • Martin-Cuadrado, A.B.; Fontaine, T.; Esteban, P.F.; del Dedo, J.E.; de Medina-Redondo, M.; del Rey, F.; Latge, J.P.; de Aldana, C.R.
    Characterization of the endo-beta-1,3-glucanase activity of S. cerevisiae Eng2 and other members of the GH81 family (2008), Fungal Genet. Biol., 45, 542-553.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 D518A no activity Saccharomyces cerevisiae
3.2.1.39 D526A enzymatic activity is similar to that of the wild type protein Saccharomyces cerevisiae
3.2.1.39 D545A enzymatic activity is similar to that of the wild type protein Saccharomyces cerevisiae
3.2.1.39 D570A no activity Saccharomyces cerevisiae
3.2.1.39 D588A no activity Saccharomyces cerevisiae
3.2.1.39 E609A no activity Saccharomyces cerevisiae
3.2.1.39 E613A no activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.39 Hg2+ 0.1 mM Saccharomyces cerevisiae
3.2.1.39 Hg2+ 0.1 mM Schizosaccharomyces pombe
3.2.1.39 additional information Ca2+, Cu2+,Fe3+, Mg2+, permanganate, and N-Br-succinimide have no effect on enzymatic activity Saccharomyces cerevisiae
3.2.1.39 additional information Ca2+, Cu2+, Fe3+, Mg2+, permanganate, and N-Br-succinimide have no effect on enzymatic activity Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Saccharomyces cerevisiae
-
-
-
3.2.1.39 Saccharomyces cerevisiae YPA84
-
-
-
3.2.1.39 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39
-
 Saccharomyces cerevisiae
3.2.1.39 CM-Sepharose Fast Flow column chromatography Schizosaccharomyces pombe

Storage Stability

EC Number Storage Stability Organism
3.2.1.39 37°C, 1 h, no loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
 Saccharomyces cerevisiae laminaribiose + laminaritriose + laminaritetraose main reaction products ?
3.2.1.39 laminarin + H2O
-
 Schizosaccharomyces pombe laminaribiose + laminaritriose + laminaritetraose main reaction products ?
3.2.1.39 laminarin + H2O
-
 Saccharomyces cerevisiae YPA84 laminaribiose + laminaritriose + laminaritetraose main reaction products ?
3.2.1.39 additional information laminaribiose and laminaritriose are no substrates, laminaritetraose is very inefficiently cleaved Saccharomyces cerevisiae ?
-
 ?
3.2.1.39 additional information laminaribiose and laminaritriose are no substrates, laminaritetraose is very inefficiently cleaved Schizosaccharomyces pombe ?
-
 ?
3.2.1.39 additional information laminaribiose and laminaritriose are no substrates, laminaritetraose is very inefficiently cleaved Saccharomyces cerevisiae YPA84 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 endo-beta-1,3-glucanase
-
 Saccharomyces cerevisiae
3.2.1.39 endo-beta-1,3-glucanase
-
 Schizosaccharomyces pombe
3.2.1.39 Eng2 protein
-
 Saccharomyces cerevisiae
3.2.1.39 Eng2 protein
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 50 55
-
 Schizosaccharomyces pombe
3.2.1.39 55
-
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 6
-
-
 Schizosaccharomyces pombe
3.2.1.39 6.5
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 4.5 7
-
 Schizosaccharomyces pombe
3.2.1.39 5.5 8.5
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 4.5
-
 activity decreases rapidly at pH lower 4.5 Schizosaccharomyces pombe
3.2.1.39 5.5
-
 activity decreases rapidly at pH lower 5.5 Saccharomyces cerevisiae