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Literature summary extracted from

  • Gunawan, C.; Satianegara, G.; Chen, A.K.; Breuer, M.; Hauer, B.; Rogers, P.L.; Rosche, B.
    Yeast pyruvate decarboxylases: variation in biocatalytic characteristics for (R)-phenylacetylcarbinol production (2007), FEMS Yeast Res., 7, 33-39.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.1 synthesis Candida utilis PDC is a stable and high productivity enzyme for the production (R)-phenylacetylcarbinol, a pharmaceutical precursor Cyberlindnera jadinii
4.1.1.1 synthesis PDC is useful for the production (R)-phenylacetylcarbinol, a pharmaceutical precursor Saccharomyces cerevisiae
4.1.1.1 synthesis PDC is useful for the production (R)-phenylacetylcarbinol, a pharmaceutical precursor Candida tropicalis
4.1.1.1 synthesis PDC is useful for the production (R)-phenylacetylcarbinol, a pharmaceutical precursor Kluyveromyces marxianus

General Stability

EC Number General Stability Organism
4.1.1.1 half-life of 3 days for both crude enzyme extract and 2 days for whole cell biomass preparations in presence of 50 mM benzaldehyde at 22°C Kluyveromyces marxianus
4.1.1.1 half-life of 5 days for both crude enzyme extract and 3.5 days for whole cell biomass preparations in presence of 50 mM benzaldehyde at 22°C Saccharomyces cerevisiae
4.1.1.1 half-life of 7 days for both crude enzyme extract and whole cell biomass preparations in presence of 50 mM benzaldehyde at 22°C, half-life of nearly 2 weeks for crude enzyme extract and slightly longer for whole cell biomass in absence of benzaldehyde Cyberlindnera jadinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.1 additional information Cyberlindnera jadinii the enzyme also performs carboligation reactions ?
-
 ?
4.1.1.1 additional information Saccharomyces cerevisiae the enzyme also performs carboligation reactions ?
-
 ?
4.1.1.1 additional information Candida tropicalis the enzyme also performs carboligation reactions ?
-
 ?
4.1.1.1 additional information Kluyveromyces marxianus the enzyme also performs carboligation reactions ?
-
 ?
4.1.1.1 additional information Saccharomyces cerevisiae UNSW 102200 the enzyme also performs carboligation reactions ?
-
 ?
4.1.1.1 additional information Kluyveromyces marxianus UNSW 510700 the enzyme also performs carboligation reactions ?
-
 ?
4.1.1.1 additional information Candida tropicalis LU57 the enzyme also performs carboligation reactions ?
-
 ?
4.1.1.1 additional information Cyberlindnera jadinii UNSW 70940 the enzyme also performs carboligation reactions ?
-
 ?
4.1.1.1 pyruvate Cyberlindnera jadinii
-
 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate Saccharomyces cerevisiae
-
 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate Candida tropicalis
-
 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate Kluyveromyces marxianus
-
 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate Saccharomyces cerevisiae UNSW 102200
-
 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate Kluyveromyces marxianus UNSW 510700
-
 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate Candida tropicalis LU57
-
 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate Cyberlindnera jadinii UNSW 70940
-
 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde Cyberlindnera jadinii
-
 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde Saccharomyces cerevisiae
-
 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde Candida tropicalis
-
 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde Kluyveromyces marxianus
-
 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde Saccharomyces cerevisiae UNSW 102200
-
 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde Kluyveromyces marxianus UNSW 510700
-
 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde Candida tropicalis LU57
-
 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde Cyberlindnera jadinii UNSW 70940
-
 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde Cyberlindnera jadinii stereospecific reaction, optimization of the biotransformation assay method (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde Saccharomyces cerevisiae stereospecific reaction, optimization of the biotransformation assay method (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde Candida tropicalis stereospecific reaction, optimization of the biotransformation assay method (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde Kluyveromyces marxianus stereospecific reaction, optimization of the biotransformation assay method (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde Saccharomyces cerevisiae UNSW 102200 stereospecific reaction, optimization of the biotransformation assay method (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde Kluyveromyces marxianus UNSW 510700 stereospecific reaction, optimization of the biotransformation assay method (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde Candida tropicalis LU57 stereospecific reaction, optimization of the biotransformation assay method (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde Cyberlindnera jadinii UNSW 70940 stereospecific reaction, optimization of the biotransformation assay method (R)-phenylacetylcarbinol + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.1 Candida tropicalis
-
-
-
4.1.1.1 Candida tropicalis LU57
-
-
-
4.1.1.1 Cyberlindnera jadinii
-
-
-
4.1.1.1 Cyberlindnera jadinii UNSW 70940
-
-
-
4.1.1.1 Kluyveromyces marxianus
-
-
-
4.1.1.1 Kluyveromyces marxianus UNSW 510700
-
-
-
4.1.1.1 Saccharomyces cerevisiae
-
-
-
4.1.1.1 Saccharomyces cerevisiae UNSW 102200
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.1 additional information
-
 (R)-phenylacetylcarbinol production rate Cyberlindnera jadinii
4.1.1.1 additional information
-
 (R)-phenylacetylcarbinol production rate Saccharomyces cerevisiae
4.1.1.1 additional information
-
 (R)-phenylacetylcarbinol production rate Candida tropicalis
4.1.1.1 additional information
-
 (R)-phenylacetylcarbinol production rate Kluyveromyces marxianus

Storage Stability

EC Number Storage Stability Organism
4.1.1.1 half-life of 3 days for crude enzyme extract and less than 1 day for whole cell biomass in absence or presence of benzaldehyde at 22°C Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.1 additional information the enzyme also performs carboligation reactions Cyberlindnera jadinii ?
-
 ?
4.1.1.1 additional information the enzyme also performs carboligation reactions Saccharomyces cerevisiae ?
-
 ?
4.1.1.1 additional information the enzyme also performs carboligation reactions Candida tropicalis ?
-
 ?
4.1.1.1 additional information the enzyme also performs carboligation reactions Kluyveromyces marxianus ?
-
 ?
4.1.1.1 additional information the enzyme also performs carboligation reactions Saccharomyces cerevisiae UNSW 102200 ?
-
 ?
4.1.1.1 additional information the enzyme also performs carboligation reactions Kluyveromyces marxianus UNSW 510700 ?
-
 ?
4.1.1.1 additional information the enzyme also performs carboligation reactions Candida tropicalis LU57 ?
-
 ?
4.1.1.1 additional information the enzyme also performs carboligation reactions Cyberlindnera jadinii UNSW 70940 ?
-
 ?
4.1.1.1 pyruvate
-
 Cyberlindnera jadinii acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 Saccharomyces cerevisiae acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 Candida tropicalis acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 Kluyveromyces marxianus acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 Saccharomyces cerevisiae UNSW 102200 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 Kluyveromyces marxianus UNSW 510700 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 Candida tropicalis LU57 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 Cyberlindnera jadinii UNSW 70940 acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde
-
 Cyberlindnera jadinii acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde
-
 Saccharomyces cerevisiae acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde
-
 Candida tropicalis acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde
-
 Kluyveromyces marxianus acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde
-
 Saccharomyces cerevisiae UNSW 102200 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde
-
 Kluyveromyces marxianus UNSW 510700 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde
-
 Candida tropicalis LU57 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + acetaldehyde
-
 Cyberlindnera jadinii UNSW 70940 acetoin + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde stereospecific reaction, optimization of the biotransformation assay method Cyberlindnera jadinii (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde stereospecific reaction, optimization of the biotransformation assay method Saccharomyces cerevisiae (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde stereospecific reaction, optimization of the biotransformation assay method Candida tropicalis (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde stereospecific reaction, optimization of the biotransformation assay method Kluyveromyces marxianus (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde stereospecific reaction, optimization of the biotransformation assay method Saccharomyces cerevisiae UNSW 102200 (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde stereospecific reaction, optimization of the biotransformation assay method Kluyveromyces marxianus UNSW 510700 (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde stereospecific reaction, optimization of the biotransformation assay method Candida tropicalis LU57 (R)-phenylacetylcarbinol + CO2
-
 ?
4.1.1.1 pyruvate + benzaldehyde stereospecific reaction, optimization of the biotransformation assay method Cyberlindnera jadinii UNSW 70940 (R)-phenylacetylcarbinol + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.1 PDC
-
 Cyberlindnera jadinii
4.1.1.1 PDC
-
 Saccharomyces cerevisiae
4.1.1.1 PDC
-
 Candida tropicalis
4.1.1.1 PDC
-
 Kluyveromyces marxianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.1 30
-
 in vivo assay at Cyberlindnera jadinii
4.1.1.1 30
-
 in vivo assay at Saccharomyces cerevisiae
4.1.1.1 30
-
 in vivo assay at Candida tropicalis
4.1.1.1 30
-
 in vivo assay at Kluyveromyces marxianus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.1 thiamine diphosphate
-
 Cyberlindnera jadinii
4.1.1.1 thiamine diphosphate
-
 Saccharomyces cerevisiae
4.1.1.1 thiamine diphosphate
-
 Candida tropicalis
4.1.1.1 thiamine diphosphate
-
 Kluyveromyces marxianus