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Literature summary extracted from

  • Ding, S.; Cao, J.; Zhou, R.; Zheng, F.
    Molecular cloning, and characterization of a modular acetyl xylan esterase from the edible straw mushroom Volvariella volvacea (2007), FEMS Microbiol. Lett., 274, 304-310.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 expression in Pichia pastoris Volvariella volvacea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.72 0.3077
-
 4-yethylumbelliferyl acetate pH 7.5, 50°C Volvariella volvacea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.72 39900
-
 x * 39900, calculated from sequence Volvariella volvacea

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Volvariella volvacea Q09IZ4
-
-
3.1.1.72 Volvariella volvacea V14 Q09IZ4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72
-
 Volvariella volvacea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.72 mycelium
-
 Volvariella volvacea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-methylumbelliferyl acetate + H2O
-
 Volvariella volvacea 4-methylumbelliferone + acetate
-
 ?
3.1.1.72 4-methylumbelliferyl acetate + H2O
-
 Volvariella volvacea V14 4-methylumbelliferone + acetate
-
 ?
3.1.1.72 acetylxylan + H2O
-
 Volvariella volvacea ?
-
 ?
3.1.1.72 acetylxylan + H2O
-
 Volvariella volvacea V14 ?
-
 ?
3.1.1.72 beta-D-xylose tetraacetate + H2O
-
 Volvariella volvacea ?
-
 ?
3.1.1.72 beta-D-xylose tetraacetate + H2O
-
 Volvariella volvacea V14 ?
-
 ?
3.1.1.72 additional information alpha-naphthyl acetate is a poor substrate for the enzyme and is hydrolyzed at only 1.8% of the rate observed with xylose tetraacetate. No activity is detectable on p-nitrophenyl acetate Volvariella volvacea ?
-
 ?
3.1.1.72 additional information alpha-naphthyl acetate is a poor substrate for the enzyme and is hydrolyzed at only 1.8% of the rate observed with xylose tetraacetate. No activity is detectable on p-nitrophenyl acetate Volvariella volvacea V14 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 ? x * 39900, calculated from sequence Volvariella volvacea

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 Vvaxe1
-
 Volvariella volvacea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 60
-
 hydrolysis of 4-methylumbelliferyl acetate Volvariella volvacea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.72 30 70 30°C: about 50% of maximal activity, 70°C: about 90% of maximal activity, hydrolysis of 4-methylumbelliferyl acetate Volvariella volvacea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 8
-
 hydrolysis of 4-methylumbelliferyl acetate Volvariella volvacea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.72 7.5 10 pH 7.5: about 40% of maximal activity, pH 10: about 40% of maximal activity, hydrolysis of 4-methylumbelliferyl acetate Volvariella volvacea