Literature summary extracted from
Tseng, C.H.; Yang, C.H.; Lin, H.J.; Wu, C.; Chen, H.P.
The S subunit of D-ornithine aminomutase from Clostridium sticklandii is responsible for the allosteric regulation in D-alpha-lysine aminomutase (2007), FEMS Microbiol. Lett., 274, 148-153.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
5.4.3.3 |
ATP |
protein KamDE comprised of the 30000 and 51000 Da subunits of the E1 component of D-alpha-lysine aminomutase is catalytically active in absence of the third 128000 Da subunit, but ATP no longer has a regulatory effect on it. The S subunit of D-ornithine aminomutase, OraS, is capable of forming a complex with KamDE and restores the enzymes ATP-dependent allosteric regulation. ATP lowers the Km value of the KamDE-OraS complex for adenosylcobalamin and pyridoxal phosphate |
Acetoanaerobium sticklandii |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.4.3.3 |
genes kamDE coding for the 30000 and 51000 Da subunits of the E1 component of D-alpha-lysine aminomutase, expression in Escherichia coli |
Acetoanaerobium sticklandii |
5.4.3.5 |
enzyme S subunit OraS, expression in Escherichia coli |
Acetoanaerobium sticklandii |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.4.3.3 |
additional information |
protein KamDE comprised of the 30000 and 51000 kDa subunits of the E1 component of D-alpha-lysine aminomutase is catalytically active in absence of the third 12800 kDa subunit, but ATP no longer has a regulatory effect on it. The S subunit of D-ornithine aminomutase, OraS, is capable of forming a complex with KamDE and restores the enzymes ATP-dependent allosteric regulation. OraS protein alone lowers the Km of KamDE for adenosylcobalamin and pyridoxal phosphate |
Acetoanaerobium sticklandii |
5.4.3.5 |
additional information |
protein KamDE comprised of the 30000 and 51000 Da subunits of the E1 component of D-alpha-lysine aminomutase is catalytically active in absence of the third 12800 kDa subunit, but ATP no longer has a regulatory effect on it. The S subunit of D-ornithine aminomutase, OraS, is capable of forming a complex with KamDE and restores the enzymes ATP-dependent allosteric regulation. OraS protein alone lowers the Km of KamDE for adenosylcobalamin and pyridoxal phosphate |
Acetoanaerobium sticklandii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.4.3.3 |
Acetoanaerobium sticklandii |
- |
- |
- |
5.4.3.5 |
Acetoanaerobium sticklandii |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.4.3.3 |
protein KamDE comprised of the 30000 and 51000 Da subunits of the E1 component of D-alpha-lysine aminomutase, expression in Escherichia coli |
Acetoanaerobium sticklandii |
5.4.3.5 |
enzyme S subunit OraS, expression in Escherichia coli |
Acetoanaerobium sticklandii |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
5.4.3.3 |
-20°C, 50% glycerol, protein KamDE comprised of the 30000 and 51000 Da subunits of the E1 component of D-alpha-lysine aminomutase can be stored for several months with significant loss of activity |
Acetoanaerobium sticklandii |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.4.3.3 |
More |
protein KamDE comprised of the 30 and 51 kDa subunits of the E1 component of D-alpha-lysine aminomutase is catalytically active in absence of the third 12.8 kDa subunit, but ATP no longer has a regulatory effect on it. The S subunit of D-ornithine aminomutase, OraS, is capable of forming a complex with KamDE and restores the enzymes ATP-dependent allosteric regulation |
Acetoanaerobium sticklandii |
5.4.3.5 |
More |
protein KamDE comprised of the 30000 and 51000 Da subunits of the E1 component of D-alpha-lysine aminomutase is catalytically active in absence of the third 12800 kDa subunit, but ATP no longer has a regulatory effect on it. The S subunit of D-ornithine aminomutase, OraS, is capable of forming a complex with KamDE and restores the enzymes ATP-dependent allosteric regulation |
Acetoanaerobium sticklandii |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
5.4.3.3 |
adenosylcobalamin |
Km value 6.1 mM, in presence of S-subunit of D-ornithine aminomutase, Km value 3.5 mM, in presence of ATP and S-subunit of D-ornithine aminomutase, Km value 1.9 mM |
Acetoanaerobium sticklandii |
|
5.4.3.3 |
pyridoxal 5'-phosphate |
Km value 3.9 mM, in presence of S-subunit of D-ornithine aminomutase, Km value 3.0 mM, in presence of ATP and S-subunit of D-ornithine aminomutase, Km value 2.4 mM |
Acetoanaerobium sticklandii |
|