EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.54 | expression in Escherichia coli and Pichia pastoris | unidentified |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.54 | 4.4 | - |
alpha-cyclodextrin | - |
unidentified | |
3.2.1.54 | 6.1 | - |
beta-cyclodextrin | - |
unidentified |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.54 | Ca2+ | required for full activity | unidentified |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 68000 | - |
SDS-PAGE, size exclusion chromatography | unidentified |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.54 | unidentified | - |
Env Cda13A can be classified into the neopullulanase subfamily consisting of neopullulanases, cyclomaltodextrinases and maltogenic amylases | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.54 | of the recombinant protein by filtration of the supernatant | unidentified |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 0.1 | - |
starch as substrate | unidentified |
3.2.1.54 | 0.3 | - |
pullulan as substrate | unidentified |
3.2.1.54 | 3.4 | - |
gamma-cyclodextrin as substrate | unidentified |
3.2.1.54 | 14.8 | - |
beta-cyclodextrin as substrate | unidentified |
3.2.1.54 | 30.7 | - |
alpha-cyclodextrin as substrate | unidentified |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.54 | alpha-cyclodextrin + H2O | - |
unidentified | maltose + D-glucose | - |
? | |
3.2.1.54 | beta-cyclodextrin + H2O | - |
unidentified | maltose + D-glucose | in an approximate molar ratio of 2 to 1 | ? | |
3.2.1.54 | cyclomaltodextrin + H2O | - |
unidentified | linear maltodextrin | - |
? | |
3.2.1.54 | gamma-cyclodextrin + H2O | - |
unidentified | maltose + D-glucose | in an approximate molar ratio of 2 to 1 | ? | |
3.2.1.54 | maltooligosaccharides + H2O | - |
unidentified | D-glucose + ? | - |
? | |
3.2.1.54 | pullulan + H2O | - |
unidentified | maltotriose + ? | - |
? | |
3.2.1.54 | starch + H2O | - |
unidentified | D-glucose + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.54 | monomer | - |
unidentified |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.54 | CDase | - |
unidentified |
3.2.1.54 | cyclomaltodextrinase | - |
unidentified |
3.2.1.54 | Env cda13A | - |
unidentified |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 50 | 55 | - |
unidentified |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.54 | 6 | 7 | - |
unidentified |