BRENDA - Enzyme Database

Cloning, expression and characterization of an extracellular enolase from Leuconostoc mesenteroides

Lee, J.H.; Kang, H.K.; Moon, Y.H.; Cho, D.L.; Kim, D.; Choe, J.Y.; Honzatko, R.; Robyt, J.F.; FEMS Microbiol. Lett. 259, 240-248 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3)pLysS using the pRSETA expression vector, recombinant His-tagged protein
Leuconostoc mesenteroides
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.11
hanging drop method, from preparations of purified dextransucrase
Leuconostoc mesenteroides
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
non-competitive inhibition without phosphate and in presence of 1 mM phosphate, competitive inhibition in presence of 20 mM phosphate
Leuconostoc mesenteroides
4.2.1.11
additional information
recombinant enolase inhibits activity of purified dextransucrase
Leuconostoc mesenteroides
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
2.61
-
2-phospho-D-glycerate
at pH 6.8
Leuconostoc mesenteroides
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47550
-
deduced from sequence
Leuconostoc mesenteroides
4.2.1.11
51400
-
SDS-PAGE
Leuconostoc mesenteroides
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Leuconostoc mesenteroides
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Leuconostoc mesenteroides B-512FMCM
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Leuconostoc mesenteroides
Q1ET73
constitutively secreting dextransucrase
-
4.2.1.11
Leuconostoc mesenteroides B-512FMCM
Q1ET73
constitutively secreting dextransucrase
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
extracellular enolase identified from isolates of extracellular dextransucrase from cultures of Leuconostoc mesenteroides, accumulation as small crystals
Leuconostoc mesenteroides
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
culture medium
strain B-512FMCM
Leuconostoc mesenteroides
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
activity of recombinant protein spectrophotometrically measured by conversion of 2-phospho-D-glycerate to phosphoenolpyruvate, substrate concentrations of 3 mM
Leuconostoc mesenteroides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
679893
Leuconostoc mesenteroides
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
679893
Leuconostoc mesenteroides B-512FMCM
phosphoenolpyruvate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
More
extracellular enolase predicted as octamer, with two of its subunits defining the asymmetric unit of the crystal
Leuconostoc mesenteroides
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
4.2.1.11
30
-
activity assay at
Leuconostoc mesenteroides
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
optimum determined by activity assay
Leuconostoc mesenteroides
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
6
8.3
catalytic activity above and below pH 6.8 remains constant at 30% of maximal activity
Leuconostoc mesenteroides
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
4.6
-
F-
in presence of 1 mM phosphate
Leuconostoc mesenteroides
4.2.1.11
5.4
-
F-
in absence of phosphate
Leuconostoc mesenteroides
4.2.1.11
9.2
-
F-
in presence of 20 mM phosphate
Leuconostoc mesenteroides
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3)pLysS using the pRSETA expression vector, recombinant His-tagged protein
Leuconostoc mesenteroides
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.11
hanging drop method, from preparations of purified dextransucrase
Leuconostoc mesenteroides
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
non-competitive inhibition without phosphate and in presence of 1 mM phosphate, competitive inhibition in presence of 20 mM phosphate
Leuconostoc mesenteroides
4.2.1.11
additional information
recombinant enolase inhibits activity of purified dextransucrase
Leuconostoc mesenteroides
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
4.6
-
F-
in presence of 1 mM phosphate
Leuconostoc mesenteroides
4.2.1.11
5.4
-
F-
in absence of phosphate
Leuconostoc mesenteroides
4.2.1.11
9.2
-
F-
in presence of 20 mM phosphate
Leuconostoc mesenteroides
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
2.61
-
2-phospho-D-glycerate
at pH 6.8
Leuconostoc mesenteroides
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47550
-
deduced from sequence
Leuconostoc mesenteroides
4.2.1.11
51400
-
SDS-PAGE
Leuconostoc mesenteroides
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Leuconostoc mesenteroides
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Leuconostoc mesenteroides B-512FMCM
-
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
extracellular enolase identified from isolates of extracellular dextransucrase from cultures of Leuconostoc mesenteroides, accumulation as small crystals
Leuconostoc mesenteroides
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
culture medium
strain B-512FMCM
Leuconostoc mesenteroides
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
activity of recombinant protein spectrophotometrically measured by conversion of 2-phospho-D-glycerate to phosphoenolpyruvate, substrate concentrations of 3 mM
Leuconostoc mesenteroides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
679893
Leuconostoc mesenteroides
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
679893
Leuconostoc mesenteroides B-512FMCM
phosphoenolpyruvate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
More
extracellular enolase predicted as octamer, with two of its subunits defining the asymmetric unit of the crystal
Leuconostoc mesenteroides
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
4.2.1.11
30
-
activity assay at
Leuconostoc mesenteroides
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
optimum determined by activity assay
Leuconostoc mesenteroides
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
6
8.3
catalytic activity above and below pH 6.8 remains constant at 30% of maximal activity
Leuconostoc mesenteroides