Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hardeman, F.; Sjoeling, S.
    Metagenomic approach for the isolation of a novel low-temperature-active lipase from uncultured bacteria of marine sediment (2007), FEMS Microbiol. Ecol., 59, 524-534.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 construction and screening of a metagenomic library, DNA and amino acid sequence determination and analysis of ORF and promoter region, overexpression using a copy control fosmid vector in Escherichia coli, the fosmid is isolated using alkaline minipreps according to the protocol for Bacterial Artificial Chromosomes minipreparations uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 0.22
-
 4-nitrophenyl butyrate pH 7.2-7.3, 10°C uncultured bacterium
3.1.1.3 0.32
-
 4-nitrophenyl butyrate pH 7.2-7.3, 20°C uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 uncultured bacterium Q3YJM9 isolated from Baltic Sea soft-bottom sediment samples
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.3 triacylglycerol + H2O = diacylglycerol + a carboxylate the enzyme possesses a putative active site, GDSAG, a catalytic triad, formed by Ser148, Glu242 and His272, and a HGG motif uncultured bacterium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 1,2-di-O-lauryl-rac-glycero-3-glutaric acid 6'-methylresorufin ester + H2O chromogenic substrate uncultured bacterium ?
-
 ?
3.1.1.3 4-nitrophenyl caprylate + H2O
-
 uncultured bacterium 4-nitrophenol + caprylate
-
 ?
3.1.1.3 4-nitrophenyl decanoate + H2O
-
 uncultured bacterium 4-nitrophenol + decanoate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 uncultured bacterium 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl myristate + H2O
-
 uncultured bacterium 4-nitrophenol + myristate
-
 ?
3.1.1.3 4-nitrophenylbutyrate + H2O
-
 uncultured bacterium 4-nitrophenol + butyrate
-
 ?
3.1.1.3 additional information the lipase hydrolyses 4-nitrophenyl esters of fatty acids with chain lengths up to C14 uncultured bacterium ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 uncultured bacterium dibutyrin + butyrate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 h1Lip1
-
 uncultured bacterium
3.1.1.3 lipase
-
 uncultured bacterium
3.1.1.3 low-temperature-active lipase
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 35
-
 substrate 4-nitrophenyl butyrate uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 5 40 33% of maximal activity at 5°C, 44% at 10°C, rapid decrease in activity above 40°C uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 25 40 h1Lip1 is unstable at 25°C and inactivated at 40°C with t1/2 of below 5 min uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7.2 7.3 assay at uncultured bacterium