EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.3 | construction and screening of a metagenomic library, DNA and amino acid sequence determination and analysis of ORF and promoter region, overexpression using a copy control fosmid vector in Escherichia coli, the fosmid is isolated using alkaline minipreps according to the protocol for Bacterial Artificial Chromosomes minipreparations | uncultured bacterium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.3 | 0.22 | - |
4-nitrophenyl butyrate | pH 7.2-7.3, 10°C | uncultured bacterium | |
3.1.1.3 | 0.32 | - |
4-nitrophenyl butyrate | pH 7.2-7.3, 20°C | uncultured bacterium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | uncultured bacterium | Q3YJM9 | isolated from Baltic Sea soft-bottom sediment samples | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.1.3 | triacylglycerol + H2O = diacylglycerol + a carboxylate | the enzyme possesses a putative active site, GDSAG, a catalytic triad, formed by Ser148, Glu242 and His272, and a HGG motif | uncultured bacterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 1,2-di-O-lauryl-rac-glycero-3-glutaric acid 6'-methylresorufin ester + H2O | chromogenic substrate | uncultured bacterium | ? | - |
? | |
3.1.1.3 | 4-nitrophenyl caprylate + H2O | - |
uncultured bacterium | 4-nitrophenol + caprylate | - |
? | |
3.1.1.3 | 4-nitrophenyl decanoate + H2O | - |
uncultured bacterium | 4-nitrophenol + decanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl laurate + H2O | - |
uncultured bacterium | 4-nitrophenol + laurate | - |
? | |
3.1.1.3 | 4-nitrophenyl myristate + H2O | - |
uncultured bacterium | 4-nitrophenol + myristate | - |
? | |
3.1.1.3 | 4-nitrophenylbutyrate + H2O | - |
uncultured bacterium | 4-nitrophenol + butyrate | - |
? | |
3.1.1.3 | additional information | the lipase hydrolyses 4-nitrophenyl esters of fatty acids with chain lengths up to C14 | uncultured bacterium | ? | - |
? | |
3.1.1.3 | tributyrin + H2O | - |
uncultured bacterium | dibutyrin + butyrate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | h1Lip1 | - |
uncultured bacterium |
3.1.1.3 | lipase | - |
uncultured bacterium |
3.1.1.3 | low-temperature-active lipase | - |
uncultured bacterium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 35 | - |
substrate 4-nitrophenyl butyrate | uncultured bacterium |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 5 | 40 | 33% of maximal activity at 5°C, 44% at 10°C, rapid decrease in activity above 40°C | uncultured bacterium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 25 | 40 | h1Lip1 is unstable at 25°C and inactivated at 40°C with t1/2 of below 5 min | uncultured bacterium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 7.2 | 7.3 | assay at | uncultured bacterium |