BRENDA - Enzyme Database

Evidence for an operative glyoxylate cycle in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius

Uhrigshardt, H.; Walden, M.; John, H.; Petersen, A.; Anemüller, S.; FEBS Lett. 513, 223-229 (2002)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
3-nitropropionate
-
Sulfolobus acidocaldarius
4.1.3.1
Itaconate
-
Sulfolobus acidocaldarius
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.72
-
isocitrate
-
Sulfolobus acidocaldarius
4.1.3.2
0.0056
-
acetyl-CoA
in 25 mM Bis-Tris-propane/HCl, 100 mM NaCl, pH 8.0 at 75°C
Sulfolobus acidocaldarius
4.1.3.2
0.044
-
glyoxylate
in 25 mM Bis-Tris-propane/HCl, 100 mM NaCl, pH 8.0 at 75°C
Sulfolobus acidocaldarius
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
dependent on Mg2+
Sulfolobus acidocaldarius
4.1.3.2
additional information
not dependent on Mg2+
Sulfolobus acidocaldarius
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
46000
-
4 * 46000, SDS-PAGE
Sulfolobus acidocaldarius
4.1.3.1
53750
-
4 * 53750, gel filtration
Sulfolobus acidocaldarius
4.1.3.1
215000
-
gel filtration
Sulfolobus acidocaldarius
4.1.3.2
88400
-
2 * 88400, SDS-PAGE
Sulfolobus acidocaldarius
4.1.3.2
103000
-
2 * 103000, gel filtration
Sulfolobus acidocaldarius
4.1.3.2
206000
-
gel filtration
Sulfolobus acidocaldarius
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Sulfolobus acidocaldarius
-
strain DSM 639
-
4.1.3.2
Sulfolobus acidocaldarius
-
strain DSM 639
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
copurified with aconitase
Sulfolobus acidocaldarius
4.1.3.2
Phenyl Sepharose HP column chromatography, Superdex 200 gel filtration, Red-Sepharose column chromatography, and Uno-Q1 column chromatography
Sulfolobus acidocaldarius
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
7.9
-
after 30fold purification
Sulfolobus acidocaldarius
4.1.3.2
57
-
after 139fold purification, in 25 mM Bis-Tris-propane/HCl, 100 mM NaCl, pH 8.0 at 75°C
Sulfolobus acidocaldarius
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
679815
Sulfolobus acidocaldarius
succinate + glyoxylate
-
-
-
?
4.1.3.2
glyoxylate + acetyl-CoA + 5,5'-dithiobis-(2-nitrobenzoic acid)
-
679815
Sulfolobus acidocaldarius
5-mercapto-2-nitrobenzoic acid + ?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
homotetramer
4 * 46000, SDS-PAGE; 4 * 53750, gel filtration
Sulfolobus acidocaldarius
4.1.3.2
homodimer
2 * 103000, gel filtration; 2 * 88400, SDS-PAGE
Sulfolobus acidocaldarius
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
80
-
-
Sulfolobus acidocaldarius
4.1.3.2
79.5
-
-
Sulfolobus acidocaldarius
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6
-
-
Sulfolobus acidocaldarius
4.1.3.2
6.8
-
-
Sulfolobus acidocaldarius
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.3.1
0.015
-
3-nitropropionate
-
Sulfolobus acidocaldarius
4.1.3.1
0.07
-
Itaconate
-
Sulfolobus acidocaldarius
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
3-nitropropionate
-
Sulfolobus acidocaldarius
4.1.3.1
Itaconate
-
Sulfolobus acidocaldarius
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.3.1
0.015
-
3-nitropropionate
-
Sulfolobus acidocaldarius
4.1.3.1
0.07
-
Itaconate
-
Sulfolobus acidocaldarius
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.72
-
isocitrate
-
Sulfolobus acidocaldarius
4.1.3.2
0.0056
-
acetyl-CoA
in 25 mM Bis-Tris-propane/HCl, 100 mM NaCl, pH 8.0 at 75°C
Sulfolobus acidocaldarius
4.1.3.2
0.044
-
glyoxylate
in 25 mM Bis-Tris-propane/HCl, 100 mM NaCl, pH 8.0 at 75°C
Sulfolobus acidocaldarius
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
dependent on Mg2+
Sulfolobus acidocaldarius
4.1.3.2
additional information
not dependent on Mg2+
Sulfolobus acidocaldarius
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
46000
-
4 * 46000, SDS-PAGE
Sulfolobus acidocaldarius
4.1.3.1
53750
-
4 * 53750, gel filtration
Sulfolobus acidocaldarius
4.1.3.1
215000
-
gel filtration
Sulfolobus acidocaldarius
4.1.3.2
88400
-
2 * 88400, SDS-PAGE
Sulfolobus acidocaldarius
4.1.3.2
103000
-
2 * 103000, gel filtration
Sulfolobus acidocaldarius
4.1.3.2
206000
-
gel filtration
Sulfolobus acidocaldarius
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
copurified with aconitase
Sulfolobus acidocaldarius
4.1.3.2
Phenyl Sepharose HP column chromatography, Superdex 200 gel filtration, Red-Sepharose column chromatography, and Uno-Q1 column chromatography
Sulfolobus acidocaldarius
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
7.9
-
after 30fold purification
Sulfolobus acidocaldarius
4.1.3.2
57
-
after 139fold purification, in 25 mM Bis-Tris-propane/HCl, 100 mM NaCl, pH 8.0 at 75°C
Sulfolobus acidocaldarius
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
679815
Sulfolobus acidocaldarius
succinate + glyoxylate
-
-
-
?
4.1.3.2
glyoxylate + acetyl-CoA + 5,5'-dithiobis-(2-nitrobenzoic acid)
-
679815
Sulfolobus acidocaldarius
5-mercapto-2-nitrobenzoic acid + ?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
homotetramer
4 * 46000, SDS-PAGE; 4 * 53750, gel filtration
Sulfolobus acidocaldarius
4.1.3.2
homodimer
2 * 103000, gel filtration; 2 * 88400, SDS-PAGE
Sulfolobus acidocaldarius
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
80
-
-
Sulfolobus acidocaldarius
4.1.3.2
79.5
-
-
Sulfolobus acidocaldarius
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6
-
-
Sulfolobus acidocaldarius
4.1.3.2
6.8
-
-
Sulfolobus acidocaldarius