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Literature summary extracted from

  • Zhao, S.; Choy, B.S.; Kornblatt, M.J.
    Effects of the G376E and G157D mutations on the stability of yeast enolase - a model for human muscle enolase deficiency (2007), FEBS J., 275, 97-106.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 inserted into pET-3a, expressed in Escherichia coli BL21-DE3, site-directed mutagenesis performed Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.11 G157D correctly folded, less stable than wild-type enolase, dissociation into subunit forms accelerated Saccharomyces cerevisiae
4.2.1.11 G376E correctly folded, less stable than wild-type enolase, dissociation into subunit forms accelerated Saccharomyces cerevisiae
4.2.1.11 additional information folding studies, dissociation experiments, determination of thermal and enzymatic stability Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Saccharomyces cerevisiae
-
 phosphoenolpyruvate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Saccharomyces cerevisiae P00924
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 recombinant proteins, SDS-PAGE Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 additional information
-
 enzyme activity monitored by following the conversion of phosphoenolpyruvate to 2-phospho-D-glycerate, specific activities of the variants, relative to wildtype enolase, are 0.1% for G157D and 0.01% for G376E Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Saccharomyces cerevisiae phosphoenolpyruvate + H2O
-
 r
4.2.1.11 2-phospho-D-glycerate influence on exchange of amino acid residues on structure, dissociation and function of enolase analyzed Saccharomyces cerevisiae phosphoenolpyruvate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 More subunit dissociation of wild-type and G157D enolases by incubation with NaClO4 at 15°C for 24 h, spectrometrically measured Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 enolase
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.11 additional information
-
 thermal stability lower in mutant forms of enolase, wild-type enolase has Tm of 55.4°C, whereas G367E and G157D reveals a Tm of 51.2°C and 49.9°C, respectively, addition of 50 microM phosphonoacetohydroxamate increases thermal stabilization of wildtype and G157D enolases, but not of G367E Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.11 7.1
-
 activity assay at Saccharomyces cerevisiae