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Literature summary extracted from

  • Herrera-Camacho, I.; Rosas-Murrieta, N.H.; Rojo-Dominguez, A.; Millan, L.; Reyes-Leyva, J.; Santos-Lopez, G.; Suarez-Rendueles, P.
    Biochemical characterization and structural prediction of a novel cytosolic leucyl aminopeptidase of the M17 family from Schizosaccharomyces pombe (2007), FEBS J., 274, 6228-6240.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline 1 mM, 38% inhibition Schizosaccharomyces pombe
3.4.11.1 Ba2+
-
 Schizosaccharomyces pombe
3.4.11.1 bestatin 100 ng/ml, 29% inhibition, competitive Schizosaccharomyces pombe
3.4.11.1 Ca2+
-
 Schizosaccharomyces pombe
3.4.11.1 Chloroquine 1 mM, 70% inhibition Schizosaccharomyces pombe
3.4.11.1 Cu2+
-
 Schizosaccharomyces pombe
3.4.11.1 EDTA 1 mM, 56% inhibition Schizosaccharomyces pombe
3.4.11.1 leupeptin 0.01 mg/ml, 12% inhibition Schizosaccharomyces pombe
3.4.11.1 Zn2+
-
 Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 3.6
-
 Lys-4-nitroanilide pH 8.5, 37°C Schizosaccharomyces pombe
3.4.11.1 3.7
-
 Arg-4-nitroanilide pH 8.5, 37°C Schizosaccharomyces pombe
3.4.11.1 4.4
-
 Met-4-nitroanilide pH 8.5, 37°C Schizosaccharomyces pombe
3.4.11.1 4.5
-
 Leu-4-nitroanilide pH 8.5, 37°C Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 cytosol
-
 Schizosaccharomyces pombe 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Mg2+ enhances activity Schizosaccharomyces pombe
3.4.11.1 Mn2+ maximal (5fold) activation at 0.5 mM or higher Schizosaccharomyces pombe
3.4.11.1 Ni2+ enhances activity Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 54000
-
 6 * 54000, SDS-PAGE Schizosaccharomyces pombe
3.4.11.1 320000
-
 gel filtration Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Schizosaccharomyces pombe Q09735
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1
-
 Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 196.9
-
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 Ala-4-nitroanilide + H2O
-
 Schizosaccharomyces pombe Ala + 4-nitroaniline
-
 ?
3.4.11.1 Arg-4-nitroanilide + H2O
-
 Schizosaccharomyces pombe Arg + 4-nitroaniline
-
 ?
3.4.11.1 Leu-4-nitroanilide + H2O
-
 Schizosaccharomyces pombe Leu + 4-nitroaniline
-
 ?
3.4.11.1 Lys-4-nitroanilide + H2O
-
 Schizosaccharomyces pombe Lys + 4-nitroaniline
-
 ?
3.4.11.1 Met-4-nitroanilide + H2O
-
 Schizosaccharomyces pombe Met + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 hexamer 6 * 54000, SDS-PAGE Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP yspII
-
 Schizosaccharomyces pombe
3.4.11.1 leucyl aminopeptidase yspII
-
 Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8.5
-
-
 Schizosaccharomyces pombe

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.1 0.00014
-
 bestatin
-
 Schizosaccharomyces pombe