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Literature summary extracted from

  • Paul, S.; Punam, S.; Chaudhuri, T.K.
    Chaperone-assisted refolding of Escherichia coli maltodextrin glucosidase (2007), FEBS J., 274, 6000-6010.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.54 biotechnology chemical or molecular operones can be used in refolding of various aggregate-prone proteins of commercial and medical importance after overexpression in Escherichia coli Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 overexpression in Escherichia coli Escherichia coli

General Stability

EC Number General Stability Organism
3.2.1.54 denaturation and unfolding induced with 20 mM sodium phosphate buffer, Escherichia coli
3.2.1.54 denatured MalZ is enhanced five-fold Escherichia coli
3.2.1.54 pH 7.0, 8 M urea and 20 mM dithiothreitol, glycerol, dimethylsulfoxide, trimethylamine-N-oxide, trehalose and proline act as chemical chaperones in the refolding of the denatured MalZ, GroEL, GroES and ATP-mediated refolding of urea Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 69000
-
 SDS-PAGE Escherichia coli

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.54 urea chemical chaperones inhibit denaturation induced by chaotropic agents such as urea Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 by Ni-chelating column HisTrap HP chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 maltodextrin + H2O
-
 Escherichia coli maltose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 monomer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 cyclomaltodextrinase
-
 Escherichia coli
3.2.1.54 maltodextrin glucosidase MalZ Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.54 42
-
 MalZ loses its native conformation and undergoes aggregation during incubation at 42°C., thermoprotection is achieved by proline, glycerol, dimethylsulfoxide or trimethylamine-N-oxide Escherichia coli