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Literature summary extracted from
- Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts (2007), FEBS J., 274, 4238-4245.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.2.1 | argininosuccinate | 0.3 mM inhibits the reaction by 97% | Arabidopsis thaliana |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.2.1 | 4.5 | - |
fumarate | - |
Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.2.1 | 2-(Nomega-L-arginino)succinate | Arabidopsis thaliana | - |
fumarate + L-arginine | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.2.1 | Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.2.1 | leaf extract is centrifuged, the supernatant lyophilized, the resuspended material is partially purified on a G-15 Sephadex and a Q-Sepharose FF column | Arabidopsis thaliana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.3.2.1 | leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.2.1 | 2-(Nomega-L-arginino)succinate | - |
Arabidopsis thaliana | fumarate + L-arginine | - |
r | |
| 4.3.2.1 | arginine + fumarate | - |
Arabidopsis thaliana | argininosuccinate | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.3.2.1 | Argininosuccinate lyase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.2.1 | 30 | - |
activity assay | Arabidopsis thaliana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.2.1 | 7.4 | - |
activity assay | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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