BRENDA - Enzyme Database

Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2

Halak, S.; Lehtioe, L.; Basta, T.; Buerger, S.; Contzen, M.; Stolz, A.; Goldman, A.; FEBS J. 273, 5169-5182 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.5.1.2
expression in Escherichia coli
Agrobacterium tumefaciens
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.5.1.2
recombinant enzyme at 2.2 A resolution. C-terminal domain present in all other members of fumarase II family is missing and enzyme contains an Arg residue as opposed to Trp in the active site. Activation of the carboxylic nucleophile by a hydrophobic environment is not required for lactonization
Agrobacterium tumefaciens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.5.1.2
citrate
noncompetitive, localization of binding pocket on the enzyme outside the active site
Agrobacterium tumefaciens
5.5.1.2
isocitrate
noncompetitive, localization of binding pocket on the enzyme outside the active site
Agrobacterium tumefaciens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.5.1.2
0.32
-
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
Agrobacterium tumefaciens
5.5.1.2
11.3
-
3-sulfomuconate
-
Agrobacterium tumefaciens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.5.1.2
37000
-
x * 37000, SDS-PAGE
Agrobacterium tumefaciens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.5.1.2
Agrobacterium tumefaciens
-
-
-
5.5.1.2
Agrobacterium tumefaciens
Q2HNZ1
-
-
5.5.1.2
Agrobacterium tumefaciens S2
Q2HNZ1
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.5.1.2
recombinant enzyme
Agrobacterium tumefaciens
Reaction
EC Number
Reaction
Commentary
Organism
5.5.1.2
2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate
activation of the carboxylic nucleophile by a hydrophobic environment is not required for lactonization
Agrobacterium tumefaciens
Storage Stability
EC Number
Storage Stability
Organism
5.5.1.2
-20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, completely stable
Agrobacterium tumefaciens
5.5.1.2
20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 50% loss of activity
Agrobacterium tumefaciens
5.5.1.2
4°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 36 days, completely stable
Agrobacterium tumefaciens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.5.1.2
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
679754
Agrobacterium tumefaciens
cis,cis-butadiene-1,2,4-tricarboxylate
-
-
-
?
5.5.1.2
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
679754
Agrobacterium tumefaciens S2
cis,cis-butadiene-1,2,4-tricarboxylate
-
-
-
?
5.5.1.2
3-sulfomuconate
-
679754
Agrobacterium tumefaciens
4-sulfomuconolactone + H2O
-
-
-
?
5.5.1.2
3-sulfomuconate
-
679754
Agrobacterium tumefaciens S2
4-sulfomuconolactone + H2O
-
-
-
?
5.5.1.2
additional information
no substrate: 3-sulfomuconate
679754
Agrobacterium tumefaciens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
5.5.1.2
?
x * 37000, SDS-PAGE
Agrobacterium tumefaciens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.5.1.2
1415
-
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
Agrobacterium tumefaciens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5.1.2
6
7
-
Agrobacterium tumefaciens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.5.1.2
7.4
-
isocitrate
-
Agrobacterium tumefaciens
5.5.1.2
18
-
citrate
-
Agrobacterium tumefaciens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.5.1.2
expression in Escherichia coli
Agrobacterium tumefaciens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.5.1.2
recombinant enzyme at 2.2 A resolution. C-terminal domain present in all other members of fumarase II family is missing and enzyme contains an Arg residue as opposed to Trp in the active site. Activation of the carboxylic nucleophile by a hydrophobic environment is not required for lactonization
Agrobacterium tumefaciens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.5.1.2
citrate
noncompetitive, localization of binding pocket on the enzyme outside the active site
Agrobacterium tumefaciens
5.5.1.2
isocitrate
noncompetitive, localization of binding pocket on the enzyme outside the active site
Agrobacterium tumefaciens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.5.1.2
7.4
-
isocitrate
-
Agrobacterium tumefaciens
5.5.1.2
18
-
citrate
-
Agrobacterium tumefaciens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.5.1.2
0.32
-
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
Agrobacterium tumefaciens
5.5.1.2
11.3
-
3-sulfomuconate
-
Agrobacterium tumefaciens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.5.1.2
37000
-
x * 37000, SDS-PAGE
Agrobacterium tumefaciens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.5.1.2
recombinant enzyme
Agrobacterium tumefaciens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
5.5.1.2
-20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, completely stable
Agrobacterium tumefaciens
5.5.1.2
20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 50% loss of activity
Agrobacterium tumefaciens
5.5.1.2
4°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 36 days, completely stable
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.5.1.2
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
679754
Agrobacterium tumefaciens
cis,cis-butadiene-1,2,4-tricarboxylate
-
-
-
?
5.5.1.2
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
679754
Agrobacterium tumefaciens S2
cis,cis-butadiene-1,2,4-tricarboxylate
-
-
-
?
5.5.1.2
3-sulfomuconate
-
679754
Agrobacterium tumefaciens
4-sulfomuconolactone + H2O
-
-
-
?
5.5.1.2
3-sulfomuconate
-
679754
Agrobacterium tumefaciens S2
4-sulfomuconolactone + H2O
-
-
-
?
5.5.1.2
additional information
no substrate: 3-sulfomuconate
679754
Agrobacterium tumefaciens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.5.1.2
?
x * 37000, SDS-PAGE
Agrobacterium tumefaciens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.5.1.2
1415
-
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
Agrobacterium tumefaciens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5.1.2
6
7
-
Agrobacterium tumefaciens