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Literature summary extracted from

  • Halak, S.; Lehtioe, L.; Basta, T.; Buerger, S.; Contzen, M.; Stolz, A.; Goldman, A.
    Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 (2006), FEBS J., 273, 5169-5182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.2 expression in Escherichia coli Agrobacterium tumefaciens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.5.1.2 recombinant enzyme at 2.2 A resolution. C-terminal domain present in all other members of fumarase II family is missing and enzyme contains an Arg residue as opposed to Trp in the active site. Activation of the carboxylic nucleophile by a hydrophobic environment is not required for lactonization Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.2 citrate noncompetitive, localization of binding pocket on the enzyme outside the active site Agrobacterium tumefaciens
5.5.1.2 isocitrate noncompetitive, localization of binding pocket on the enzyme outside the active site Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.2 0.32
-
 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
 Agrobacterium tumefaciens
5.5.1.2 11.3
-
 3-sulfomuconate
-
 Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.2 37000
-
 x * 37000, SDS-PAGE Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.2 Agrobacterium tumefaciens
-
-
-
5.5.1.2 Agrobacterium tumefaciens Q2HNZ1
-
-
5.5.1.2 Agrobacterium tumefaciens S2 Q2HNZ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.2 recombinant enzyme Agrobacterium tumefaciens

Reaction

EC Number Reaction Comment Organism Reaction ID
5.5.1.2 cis,cis-butadiene-1,2,4-tricarboxylate = 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate activation of the carboxylic nucleophile by a hydrophobic environment is not required for lactonization Agrobacterium tumefaciens
a

Storage Stability

EC Number Storage Stability Organism
5.5.1.2 -20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, completely stable Agrobacterium tumefaciens
5.5.1.2 20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 50% loss of activity Agrobacterium tumefaciens
5.5.1.2 4°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 36 days, completely stable Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.2 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
 Agrobacterium tumefaciens cis,cis-butadiene-1,2,4-tricarboxylate
-
 ?
5.5.1.2 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
 Agrobacterium tumefaciens S2 cis,cis-butadiene-1,2,4-tricarboxylate
-
 ?
5.5.1.2 3-sulfomuconate
-
 Agrobacterium tumefaciens 4-sulfomuconolactone + H2O
-
 ?
5.5.1.2 3-sulfomuconate
-
 Agrobacterium tumefaciens S2 4-sulfomuconolactone + H2O
-
 ?
5.5.1.2 additional information no substrate: 3-sulfomuconate Agrobacterium tumefaciens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.2 ? x * 37000, SDS-PAGE Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.2 1415
-
 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
 Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.2 6 7
-
 Agrobacterium tumefaciens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.5.1.2 7.4
-
 isocitrate
-
 Agrobacterium tumefaciens
5.5.1.2 18
-
 citrate
-
 Agrobacterium tumefaciens