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Literature summary extracted from

  • Shahi, P.; Kumar, I.; Sharma, R.; Sanger, S.; Jolly, R.S.
    Characterization of a novel long-chain acyl-CoA thioesterase from Alcaligenes faecalis (2006), FEBS J., 273, 2374-2387.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.20 diethyl dicarbonate 1 mM, complete inhibition Alcaligenes faecalis
3.1.2.20 dimethyl (2-hydroxy-5-nitrobenzyl) sulfonium bromide 1 mM, 11% inhibition Alcaligenes faecalis
3.1.2.20 dithiothreitol 1 mM, 20% inhibition Alcaligenes faecalis
3.1.2.20 N-bromosuccinimide 1 mM, complete inhibition Alcaligenes faecalis
3.1.2.20 p-chloromercuribenzoate 1 mM, 12% inhibition Alcaligenes faecalis
3.1.2.20 picrylsulfonic acid 1 mM, 11% inhibition Alcaligenes faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.20 0.00266
-
 myristoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
3.1.2.20 0.00284
-
 cis-vaccenoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
3.1.2.20 0.00339
-
 myristoleoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
3.1.2.20 0.00348
-
 lauroyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
3.1.2.20 0.00359
-
 stearoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
3.1.2.20 0.00385
-
 palmitoyl-CoA 20°C, pH 7.6 Alcaligenes faecalis
3.1.2.20 0.0039
-
 palmitoleoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
3.1.2.20 0.00411
-
 palmitoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
3.1.2.20 0.00551
-
 cis-vaccenoyl-CoA 20°C, pH 7.6 Alcaligenes faecalis
3.1.2.20 0.0072
-
 palmitoleoyl-CoA 20°C, pH 7.6 Alcaligenes faecalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.20 cell surface exclusively associated with Alcaligenes faecalis 9986
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.20 22000
-
 2 * 22000 Alcaligenes faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.20 Alcaligenes faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.20
-
 Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.20 cis-vaccenoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + cis-vaccenic acid
-
 ?
3.1.2.20 lauroyl-CoA + H2O
-
 Alcaligenes faecalis CoA + lauric acid
-
 ?
3.1.2.20 additional information long-chain acyl-CoAs are better substrates for thioesterase than p-nitrophenyl esters Alcaligenes faecalis ?
-
 ?
3.1.2.20 myristoleoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + myristoleic acid
-
 ?
3.1.2.20 myristoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + myristic acid
-
 ?
3.1.2.20 p-nitrophenyl acetate + H2O 30.14% of the activity with p-nitrophenyl propanoate Alcaligenes faecalis p-nitrophenol + acetate
-
 ?
3.1.2.20 p-nitrophenyl butanoate + H2O 51% of the activity with p-nitrophenyl propanoate Alcaligenes faecalis p-nitrophenol + butanoate
-
 ?
3.1.2.20 p-nitrophenyl dodecanoate + H2O 8.7% of the activity with p-nitrophenyl propanoate Alcaligenes faecalis p-nitrophenol + dodecanoate
-
 ?
3.1.2.20 p-nitrophenyl hexanoate + H2O 33% of the activity with p-nitrophenyl propanoate Alcaligenes faecalis p-nitrophenol + hexanoate
-
 ?
3.1.2.20 p-nitrophenyl propanoate + H2O
-
 Alcaligenes faecalis p-nitrophenol + propanoate
-
 ?
3.1.2.20 palmitoleoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + palmitoleic acid
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + palmitic acid
-
 ?
3.1.2.20 stearoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.20 dimer 2 * 22000 Alcaligenes faecalis

Synonyms

EC Number Synonyms Comment Organism
3.1.2.20 long-chain acyl-CoA thioesterase
-
 Alcaligenes faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.20 65
-
-
 Alcaligenes faecalis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.2.20 25 75 25°C: about 65% of maximal activity, 75°C: about 60% of maximal activity Alcaligenes faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.2.20 50
-
 3 h, no loss of activity Alcaligenes faecalis
3.1.2.20 60
-
 3 h, about 5% loss of activity Alcaligenes faecalis
3.1.2.20 70
-
 3 h, about 30% loss of activity Alcaligenes faecalis
3.1.2.20 80
-
 3 h, complete loss of activity Alcaligenes faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.20 10.5
-
-
 Alcaligenes faecalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.20 6 8 pH 6: about 40% of maximal activity, pH 6.5-8: about 80% of maximal activity Alcaligenes faecalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.2.20 5.5
-
 30°C, 20 h, no loss of activity Alcaligenes faecalis
3.1.2.20 7
-
 30°C, 20 h, about 20% loss of activity Alcaligenes faecalis
3.1.2.20 8
-
 30°C, 20 h, about 30% loss of activity Alcaligenes faecalis
3.1.2.20 9
-
 30°C, 20 h, about 45% loss of activity Alcaligenes faecalis
3.1.2.20 10
-
 30°C, 20 h, about 75% loss of activity Alcaligenes faecalis