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Literature summary extracted from

  • Ettema, T.J.; Ahmed, H.; Geerling, A.C.; van der Oost, J.; Siebers, B.
    The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) of Sulfolobus solfataricus: a key-enzyme of the semi-phosphorylative branch of the Entner-Doudoroff pathway. (2007), Extremophiles, 12, 75-88.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.9 6-O-phosphono-D-fructofuranose minor stimulatory effect Saccharolobus solfataricus
1.2.1.9 AMP minor stimulatory effect Saccharolobus solfataricus
1.2.1.9 D-fructose minor stimulatory effect Saccharolobus solfataricus
1.2.1.9 D-glucose 1-phosphate 14fold activation at 0.1 mM D-glucose 1-phosphate Saccharolobus solfataricus
1.2.1.9 pyruvate minor stimulatory effect Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.9 cloned and expressed in BL21 (DE3) CodonPlus using the pET expression vector system Saccharolobus solfataricus
1.2.1.9 expressed in Escherichia coli BL21(DE3) cells Saccharolobus solfataricus
1.2.1.9 expressed in Escherichia coli BL21(DE3) CodonPlus cells Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.9 ATP slight inhibition Saccharolobus solfataricus
1.2.1.9 galactonate slight inhibition; slight inhibition (1.2 to 1.3fold); slight inhibition (1.2 to 1.3fold) Saccharolobus solfataricus
1.2.1.9 gluconate slight inhibition Saccharolobus solfataricus
1.2.1.9 additional information glyceraldehyde and ADP have no effect on GAPN activity; not inhibited by glyceraldehyde and ADP; not inhibited by glyceraldehyde and ADP Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.9 0.09
-
 NADP+ 70°C, pH 7 Saccharolobus solfataricus
1.2.1.9 0.09
-
 NADP+ in 90 mM HEPES/KOH, 160 mM KCl, pH 7.0, at 70°C Saccharolobus solfataricus
1.2.1.9 0.09
-
 NADP+ in the absence or presence of D-glucose 1-phosphate Saccharolobus solfataricus
1.2.1.9 0.501
-
 DL-glyceraldehyde 3-phosphate in the presence of D-glucose 1-phosphate Saccharolobus solfataricus
1.2.1.9 0.51
-
 DL-glyceraldehyde 3-phosphate 70°C, pH 7 Saccharolobus solfataricus
1.2.1.9 0.51
-
 D-Glyceraldehyde-3-phosphate in 90 mM HEPES/KOH, 160 mM KCl, pH 7.0, at 70°C Saccharolobus solfataricus
1.2.1.9 0.51
-
 DL-glyceraldehyde 3-phosphate in the absence of D-glucose 1-phosphate Saccharolobus solfataricus
1.2.1.9 17.4
-
 NAD+ 70°C, pH 7 Saccharolobus solfataricus
1.2.1.9 17.4
-
 NAD+ in 90 mM HEPES/KOH, 160 mM KCl, pH 7.0, at 70°C Saccharolobus solfataricus
1.2.1.9 17.4
-
 NAD+ in the absence of D-glucose 1-phosphate Saccharolobus solfataricus
1.2.1.9 21.1
-
 NAD+ in the presence of D-glucose 1-phosphate Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.9 56930
-
 subunit, calculated from amino acid sequence Saccharolobus solfataricus
1.2.1.9 57000
-
 subunit, SDS-PAGE Saccharolobus solfataricus
1.2.1.9 57000
-
 x * 57000, SDS-PAGE Saccharolobus solfataricus
1.2.1.9 57000
-
 4 * 57000, SDS-PAGE Saccharolobus solfataricus
1.2.1.9 189000
-
-
 Saccharolobus solfataricus
1.2.1.9 189000
-
 native enzyme Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.9 D-glyceraldehyde 3-phosphate + NADP+ + H2O Saccharolobus solfataricus the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase is a key-enzyme of the semi-phosphorylative branch of the Entner–Doudoroff pathway 3-phospho-D-glycerate + NADPH + 2 H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.9 Saccharolobus solfataricus
-
-
-
1.2.1.9 Saccharolobus solfataricus Q97U30
-
-
1.2.1.9 Saccharolobus solfataricus Q97XA5 isozyme Gap-N2
-
1.2.1.9 Saccharolobus solfataricus Q97XS9 isozyme GapN-1
-
1.2.1.9 Saccharolobus solfataricus P2 Q97U30
-
-
1.2.1.9 Saccharolobus solfataricus P2 Q97XA5 isozyme Gap-N2
-
1.2.1.9 Saccharolobus solfataricus P2 Q97XS9 isozyme GapN-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.9 heat precipitation and Superdex 200 gel filtration Saccharolobus solfataricus
1.2.1.9 Superdex 200 gel filtration Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.9 additional information
-
 the recombinant enzyme has a specific activity of 3.74 U/mg Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.9 D-glyceraldehyde 3-phosphate + NAD+ + H2O enzyme shows negligible activity with NAD+. The NAD+-dependent reaction shows no saturation at NAD+ concentrations up to 50 mM. The highest enzyme activity is observed at 50 mM NAD+ which is 7fold to 8fold lower than the Vmax observed using NADP+ as cofactor Saccharolobus solfataricus 3-phospho-D-glycerate + NADH + H+
-
 ir
1.2.1.9 D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
 Saccharolobus solfataricus 3-phospho-D-glycerate + NADPH + H+
-
 ir
1.2.1.9 D-glyceraldehyde 3-phosphate + NADP+ + H2O the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase is a key-enzyme of the semi-phosphorylative branch of the Entner–Doudoroff pathway Saccharolobus solfataricus 3-phospho-D-glycerate + NADPH + 2 H+
-
 ir
1.2.1.9 D-glyceraldehyde-3-phosphate + NAD+ weak reaction Saccharolobus solfataricus D-3-phosphoglycerate + NADH + H+
-
 ir
1.2.1.9 D-glyceraldehyde-3-phosphate + NAD+ weak reaction Saccharolobus solfataricus P2 D-3-phosphoglycerate + NADH + H+
-
 ir
1.2.1.9 D-glyceraldehyde-3-phosphate + NADP+
-
 Saccharolobus solfataricus D-3-phosphoglycerate + NADPH + H+
-
 ir
1.2.1.9 D-glyceraldehyde-3-phosphate + NADP+
-
 Saccharolobus solfataricus P2 D-3-phosphoglycerate + NADPH + H+
-
 ir
1.2.1.9 DL-glyceraldehyde 3-phosphate + NAD+ + H2O NADP+ is much more efficient than NAD+ Saccharolobus solfataricus 3-phospho-DL-glycerate + NADH + H+
-
 ir
1.2.1.9 DL-glyceraldehyde 3-phosphate + NAD+ + H2O NADP+ is much more efficient than NAD+ Saccharolobus solfataricus P2 3-phospho-DL-glycerate + NADH + H+
-
 ir
1.2.1.9 DL-glyceraldehyde 3-phosphate + NADP+ + H2O
-
 Saccharolobus solfataricus 3-phospho-DL-glycerate + NADPH + H+
-
 ir
1.2.1.9 DL-glyceraldehyde 3-phosphate + NADP+ + H2O
-
 Saccharolobus solfataricus P2 3-phospho-DL-glycerate + NADPH + H+
-
 ir
1.2.1.9 DL-glyceraldehyde 3-phosphate + NADP+ + H2O
-
 Saccharolobus solfataricus 3-phospho-D-glycerate + NADPH + H+
-
 r
1.2.1.9 additional information the enzyme shows only negligible activity with NAD+ Saccharolobus solfataricus ?
-
 ?
1.2.1.9 additional information the enzyme shows only negligible activity with NAD+ Saccharolobus solfataricus P2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.9 ? x * 57000, SDS-PAGE Saccharolobus solfataricus
1.2.1.9 homotetramer 4 * 57000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.2.1.9 GAP dehydrogenase
-
 Saccharolobus solfataricus
1.2.1.9 GAPN
-
 Saccharolobus solfataricus
1.2.1.9 non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
 Saccharolobus solfataricus
1.2.1.9 non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase GAPN
-
 Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.9 NAD+ enzyme shows negligible activity with NAD+. The NAD+-dependent reaction of Sso-GAPN shows no saturation at NAD+ concentrations up to 50 mM. The highest enzyme activity is observed at 50 mM NAD+ which is 7fold to 8fold lower than the Vmax observed using NADP+ as cofactor Saccharolobus solfataricus
1.2.1.9 NAD+ negligible activity with NAD+ Saccharolobus solfataricus
1.2.1.9 NADP+
-
 Saccharolobus solfataricus
1.2.1.9 NADP+ prefers NADP+ more than NAD+ as cofactor Saccharolobus solfataricus
1.2.1.9 NADP+ the enzyme shows only negligible activity with NAD+ compared to the physiological cosubstrate NADP+ Saccharolobus solfataricus