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Literature summary extracted from

  • Cobucci-Ponzano, B.; Conte, F.; Rossi, M.; Moracci, M.
    The alpha-L: -fucosidase from Sulfolobus solfataricus (2008), Extremophiles, 12, 61-68.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.51 expressed in Escherichia coli Saccharolobus solfataricus
3.2.1.51 expression in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.51
-
 Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.51 D124G activity is not significantly affected Saccharolobus solfataricus
3.2.1.51 D124G kcat/KM for 4-nitrophenyl-alpha-L-fucopyranoside is 3.7fold lower than wild-type value Saccharolobus solfataricus
3.2.1.51 D146G activity is not significantly affected Saccharolobus solfataricus
3.2.1.51 D146G kcat/KM for 4-nitrophenyl-alpha-L-fucopyranoside is 1.5fold lower than wild-type value Saccharolobus solfataricus
3.2.1.51 D242G inactive, reactivating in the presence of sodium azide or sodium formate, reactivated enzyme maintains its thermophilicity Saccharolobus solfataricus
3.2.1.51 D242G turnover number on 4-nitrophenyl-alpha-L-fucopyranoside is 0.0012times that of the wild type activity, 40fold reactivation by azide. The fucosyl-azide product obtained by the D242G mutant is in the inverted (beta-L-) configuration when compared with the substrate Saccharolobus solfataricus
3.2.1.51 E292G affects catalysis severely, unchanged affinity for 4-nitrophenyl-alpha-L-fucopyranoside but a 154fold reduction in the turnover number Saccharolobus solfataricus
3.2.1.51 E292G kcat/KM for 4-nitrophenyl-alpha-L-fucopyranoside is 311fold lower than wild-type value Saccharolobus solfataricus
3.2.1.51 E58G affects catalysis severely Saccharolobus solfataricus
3.2.1.51 E58G kcat/KM for 4-nitrophenyl-alpha-L-fucopyranoside is 5395fold lower than wild-type value Saccharolobus solfataricus
3.2.1.51 H123G affinity for the substrate is remarkably different from that of the wild-type Saccharolobus solfataricus
3.2.1.51 H123G kcat/KM for 4-nitrophenyl-alpha-L-fucopyranoside is 2929fold lower than wild-type value Saccharolobus solfataricus
3.2.1.51 H46G affinity for the substrate is remarkably different from that of the wild-type, 607fold increase in the KM Saccharolobus solfataricus
3.2.1.51 H46G kcat/KM for 4-nitrophenyl-alpha-L-fucopyranoside is 410fold lower than wild-type value Saccharolobus solfataricus
3.2.1.51 additional information mutations of the catalytic residues with non-nucleophilic amino acids lead to strong reduction or even abolition of the enzymatic activity. Mutants can be reactivated in the presence of external nucleophiles such as sodium azide Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.028
-
 4-nitrophenyl-alpha-L-fucopyranoside wild-type enzyme Saccharolobus solfataricus
3.2.1.51 0.028
-
 4-nitrophenyl-alpha-L-fucopyranoside wild-type, at pH 6.3, in the presence of sodium phosphate Saccharolobus solfataricus
3.2.1.51 0.033
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant D146G Saccharolobus solfataricus
3.2.1.51 0.033
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant enzyme D146G Saccharolobus solfataricus
3.2.1.51 0.06
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E292G, at pH 6.3, in the presence of sodium phosphate Saccharolobus solfataricus
3.2.1.51 0.06
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant enzyme E292G Saccharolobus solfataricus
3.2.1.51 0.09
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant D124G Saccharolobus solfataricus
3.2.1.51 0.09
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E292G, at pH 4.6, in the presence of sodium acetate Saccharolobus solfataricus
3.2.1.51 0.09
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant enzyme D124G Saccharolobus solfataricus
3.2.1.51 0.26
-
 4-nitrophenyl-alpha-L-fucopyranoside wild-type, at pH 5.0, in the presence of sodium citrate Saccharolobus solfataricus
3.2.1.51 0.6
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E58G, at pH 4.6, in the presence of sodium acetate Saccharolobus solfataricus
3.2.1.51 1.1
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E58G, at pH 4.6, in the presence of sodium formate Saccharolobus solfataricus
3.2.1.51 1.6
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E58G, at pH 4.6, in the presence of sodium citrate Saccharolobus solfataricus
3.2.1.51 2.9
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E58G, at pH 6.3, in the presence of sodium phosphate and NaN3 Saccharolobus solfataricus
3.2.1.51 17
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant enzyme H46G Saccharolobus solfataricus
3.2.1.51 17
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant H46G Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 57000
-
 9 * 57000 Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Saccharolobus solfataricus
-
-
-
3.2.1.51 Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + alpha-L-fucose
-
 ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O catalytic residues Asp242, Glu58, and Glu292 Saccharolobus solfataricus 4-nitrophenol + alpha-L-fucose-(1-3)-D-GlcNAc
-
 ?
3.2.1.51 alpha-L-Fuc-(1-3)-a-L-Fuc-O-4-NP + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.51 alpha-L-Fuc-(1-3)-alpha-L-Fuc-O-4-nitrophenyl ester + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.51 additional information behaviour of the catalytic residues is different from that of Sulfolobus solfataricus alpha-fuc Thermotoga maritima ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 nonamer 9 * 57000 Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.51 alpha-fuc
-
 Thermotoga maritima
3.2.1.51 alpha-fuc encoded by an interrupted gene Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 95
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 80
-
 2 h, 40% loss of activity Saccharolobus solfataricus
3.2.1.51 80
-
 displays high stability maintaining 60% of the residual activity after 2 h Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.51 0.24
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant D242G Saccharolobus solfataricus
3.2.1.51 0.24
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant enzyme D242G Saccharolobus solfataricus
3.2.1.51 1.86
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E292G at pH 6.3 in the presence of sodium phosphate or at pH 4.6 in the presence of sodium acetate Saccharolobus solfataricus
3.2.1.51 1.86
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant enzyme E292G Saccharolobus solfataricus
3.2.1.51 9.66
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant D242G, in the presence of 2 M sodium azide Saccharolobus solfataricus
3.2.1.51 143
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E58G, at pH 4.6, in the presence of sodium citrate Saccharolobus solfataricus
3.2.1.51 224
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant D146G Saccharolobus solfataricus
3.2.1.51 224
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant enzyme D146G Saccharolobus solfataricus
3.2.1.51 240
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant D124G Saccharolobus solfataricus
3.2.1.51 240
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant enzyme D124G Saccharolobus solfataricus
3.2.1.51 287
-
 4-nitrophenyl-alpha-L-fucopyranoside wild-type enzyme Saccharolobus solfataricus
3.2.1.51 287
-
 4-nitrophenyl-alpha-L-fucopyranoside wild-type, at pH 6.3, in the presence of sodium phosphate Saccharolobus solfataricus
3.2.1.51 419
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant enzyme H46G Saccharolobus solfataricus
3.2.1.51 419
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant H46G Saccharolobus solfataricus
3.2.1.51 430
-
 4-nitrophenyl-alpha-L-fucopyranoside wild-type, at pH 5.0 Saccharolobus solfataricus
3.2.1.51 586
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E58G, at pH 4.6, in the presence of sodium acetate Saccharolobus solfataricus
3.2.1.51 679
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E58G, at pH 6.3, in the presence of sodium phosphate and NaN3 Saccharolobus solfataricus
3.2.1.51 846
-
 4-nitrophenyl-alpha-L-fucopyranoside mutant E58G, at pH 4.6, in the presence of sodium formate Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.51 4.6 6.3
-
 Saccharolobus solfataricus