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Literature summary extracted from

  • Sato, Y.; Watanabe, S.; Yamaoka, N.; Takada, Y.
    Gene cloning of cold-adapted isocitrate lyase from a psychrophilic bacterium, Colwellia psychrerythraea, and analysis of amino acid residues involved in cold adaptation of this enzyme (2008), Extremophiles, 12, 107-117.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 expressed in Escherichia coli Top10 cells Colwellia psychrerythraea

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.1 A214S increased thermostability and low specific activity at low temperature Colwellia psychrerythraea
4.1.3.1 A231E more thermolabile than the wild type enzyme Colwellia psychrerythraea
4.1.3.1 A341N decreased activity compared to the wild type enzyme Colwellia psychrerythraea
4.1.3.1 F333L very low activity Colwellia psychrerythraea
4.1.3.1 Q47K activity similar to the wild type enzyme Colwellia psychrerythraea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 58131
-
 4 * 58131, calculated from amino acid sequence Colwellia psychrerythraea
4.1.3.1 60000
-
 4 * 60000, gel filtration Colwellia psychrerythraea
4.1.3.1 240000
-
 gel filtration Colwellia psychrerythraea

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Colwellia psychrerythraea A8C5K8
-
-
4.1.3.1 Colwellia psychrerythraea NRC 1004 A8C5K8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Colwellia psychrerythraea succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Colwellia psychrerythraea NRC 1004 succinate + glyoxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 homotetramer 4 * 58131, calculated from amino acid sequence Colwellia psychrerythraea
4.1.3.1 homotetramer 4 * 60000, gel filtration Colwellia psychrerythraea

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 ICL
-
 Colwellia psychrerythraea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.1 25
-
-
 Colwellia psychrerythraea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.1 30
-
 the enzyme is rapidly inactivated at 30°C Colwellia psychrerythraea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.1 13.3
-
 isocitrate mutant enzyme A341N, at 25°C Colwellia psychrerythraea
4.1.3.1 26.6
-
 isocitrate mutant enzyme Q47K, at 25°C Colwellia psychrerythraea
4.1.3.1 28.5
-
 isocitrate wild type enzyme, at 25°C Colwellia psychrerythraea