BRENDA - Enzyme Database

Gene cloning of cold-adapted isocitrate lyase from a psychrophilic bacterium, Colwellia psychrerythraea, and analysis of amino acid residues involved in cold adaptation of this enzyme

Sato, Y.; Watanabe, S.; Yamaoka, N.; Takada, Y.; Extremophiles 12, 107-117 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli Top10 cells
Colwellia psychrerythraea
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.1.3.1
A214S
increased thermostability and low specific activity at low temperature
Colwellia psychrerythraea
4.1.3.1
A231E
more thermolabile than the wild type enzyme
Colwellia psychrerythraea
4.1.3.1
A341N
decreased activity compared to the wild type enzyme
Colwellia psychrerythraea
4.1.3.1
F333L
very low activity
Colwellia psychrerythraea
4.1.3.1
Q47K
activity similar to the wild type enzyme
Colwellia psychrerythraea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
58131
-
4 * 58131, calculated from amino acid sequence
Colwellia psychrerythraea
4.1.3.1
60000
-
4 * 60000, gel filtration
Colwellia psychrerythraea
4.1.3.1
240000
-
gel filtration
Colwellia psychrerythraea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Colwellia psychrerythraea
A8C5K8
-
-
4.1.3.1
Colwellia psychrerythraea NRC 1004
A8C5K8
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
679717
Colwellia psychrerythraea
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
679717
Colwellia psychrerythraea NRC 1004
succinate + glyoxylate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
homotetramer
4 * 58131, calculated from amino acid sequence; 4 * 60000, gel filtration
Colwellia psychrerythraea
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.3.1
25
-
-
Colwellia psychrerythraea
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.1.3.1
30
-
the enzyme is rapidly inactivated at 30C
Colwellia psychrerythraea
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.3.1
13.3
-
isocitrate
mutant enzyme A341N, at 25C
Colwellia psychrerythraea
4.1.3.1
26.6
-
isocitrate
mutant enzyme Q47K, at 25C
Colwellia psychrerythraea
4.1.3.1
28.5
-
isocitrate
wild type enzyme, at 25C
Colwellia psychrerythraea
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli Top10 cells
Colwellia psychrerythraea
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.1.3.1
A214S
increased thermostability and low specific activity at low temperature
Colwellia psychrerythraea
4.1.3.1
A231E
more thermolabile than the wild type enzyme
Colwellia psychrerythraea
4.1.3.1
A341N
decreased activity compared to the wild type enzyme
Colwellia psychrerythraea
4.1.3.1
F333L
very low activity
Colwellia psychrerythraea
4.1.3.1
Q47K
activity similar to the wild type enzyme
Colwellia psychrerythraea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
58131
-
4 * 58131, calculated from amino acid sequence
Colwellia psychrerythraea
4.1.3.1
60000
-
4 * 60000, gel filtration
Colwellia psychrerythraea
4.1.3.1
240000
-
gel filtration
Colwellia psychrerythraea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
679717
Colwellia psychrerythraea
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
679717
Colwellia psychrerythraea NRC 1004
succinate + glyoxylate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
homotetramer
4 * 58131, calculated from amino acid sequence; 4 * 60000, gel filtration
Colwellia psychrerythraea
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.3.1
25
-
-
Colwellia psychrerythraea
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.1.3.1
30
-
the enzyme is rapidly inactivated at 30C
Colwellia psychrerythraea
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.3.1
13.3
-
isocitrate
mutant enzyme A341N, at 25C
Colwellia psychrerythraea
4.1.3.1
26.6
-
isocitrate
mutant enzyme Q47K, at 25C
Colwellia psychrerythraea
4.1.3.1
28.5
-
isocitrate
wild type enzyme, at 25C
Colwellia psychrerythraea