BRENDA - Enzyme Database

Characterization of cofactor-dependent and cofactor-independent phosphoglycerate mutases from Archaea

Johnsen, U.; Schoenheit, P.; Extremophiles 11, 647-657 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.12
2,3-bisphosphoglycerate
strictly required, Km value 0.017 mM
Archaeoglobus fulgidus
5.4.2.12
2,3-bisphosphoglycerate
strictly required, Km value 0.017 mM
Thermoplasma acidophilum
Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli
Thermoplasma acidophilum
5.4.2.12
expression in Escherichia coli with His-tag
Archaeoglobus fulgidus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.4.2.12
H23A
no enzymic activity. Like wild-type, mutant forms a dimer
Thermoplasma acidophilum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
vanadate
up to 0.1 mM, 90% maximal inhibition. Inhibition may be partially reversed by EDTA
Thermoplasma acidophilum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
0.2
-
2-phospho-D-glycerate
pH 7.0, 50C
Archaeoglobus fulgidus
5.4.2.12
1.1
-
2-phospho-D-glycerate
pH 7.0, 50C
Thermoplasma acidophilum
5.4.2.12
3.3
-
3-phospho-D-glycerate
pH 7.0, 50C
Archaeoglobus fulgidus
5.4.2.12
3.3
-
3-phospho-D-glycerate
pH 7.0, 50C
Thermoplasma acidophilum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Mg2+
Mn2+ and Mg2+ are required for thermal stability
Archaeoglobus fulgidus
5.4.2.12
Mn2+
in presence of 5 mM Mg2+, 2-3fold stimulation by 0.05 mM Mn2+. Mn2+ and Mg2+ are required for thermal stability
Archaeoglobus fulgidus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
23800
-
2 * 23800, calculated, 2 * 24000, SDS-PAGE
Thermoplasma acidophilum
5.4.2.12
24000
-
2 * 23800, calculated, 2 * 24000, SDS-PAGE
Thermoplasma acidophilum
5.4.2.12
44100
-
1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein
Archaeoglobus fulgidus
5.4.2.12
46000
-
gel filtration
Archaeoglobus fulgidus
5.4.2.12
46000
-
gel filtration
Thermoplasma acidophilum
5.4.2.12
48000
-
1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein
Archaeoglobus fulgidus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Archaeoglobus fulgidus
-
cofactor-independent isoform
-
5.4.2.12
Thermoplasma acidophilum
Q9HL27
cofactor-dependent isoform
-
Renatured (Commentary)
EC Number
Commentary
Organism
5.4.2.12
inhibition of enzyme by vanadate may be partially reversed by EDTA
Thermoplasma acidophilum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.4.2.12
3.3
-
pH 7.0, 50C
Archaeoglobus fulgidus
5.4.2.12
12
-
pH 7.0, 50C
Thermoplasma acidophilum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
679715
Thermoplasma acidophilum
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
679715
Archaeoglobus fulgidus
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
679715
Thermoplasma acidophilum
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
679715
Archaeoglobus fulgidus
2-phospho-D-glycerate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
5.4.2.12
dimer
2 * 23800, calculated, 2 * 24000, SDS-PAGE
Thermoplasma acidophilum
5.4.2.12
monomer
1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein
Archaeoglobus fulgidus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
5.4.2.12
60
-
120 min, no loss of activity
Archaeoglobus fulgidus
5.4.2.12
70
-
half-life 150 min in presence of Mn2+ and Mg2+. Half-life 30 min in the absence of Mn2+ and Mg2+
Archaeoglobus fulgidus
5.4.2.12
85
-
half-life 15 min in presence of Mn2+ and Mg2+
Archaeoglobus fulgidus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
7.1
-
-
Archaeoglobus fulgidus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.4.2.12
6
-
about 40% of maximal activity
Archaeoglobus fulgidus
5.4.2.12
7.7
-
about 70% of maximal activity
Archaeoglobus fulgidus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.12
2,3-bisphosphoglycerate
strictly required, Km value 0.017 mM
Archaeoglobus fulgidus
5.4.2.12
2,3-bisphosphoglycerate
strictly required, Km value 0.017 mM
Thermoplasma acidophilum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli
Thermoplasma acidophilum
5.4.2.12
expression in Escherichia coli with His-tag
Archaeoglobus fulgidus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.4.2.12
H23A
no enzymic activity. Like wild-type, mutant forms a dimer
Thermoplasma acidophilum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
vanadate
up to 0.1 mM, 90% maximal inhibition. Inhibition may be partially reversed by EDTA
Thermoplasma acidophilum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
0.2
-
2-phospho-D-glycerate
pH 7.0, 50C
Archaeoglobus fulgidus
5.4.2.12
1.1
-
2-phospho-D-glycerate
pH 7.0, 50C
Thermoplasma acidophilum
5.4.2.12
3.3
-
3-phospho-D-glycerate
pH 7.0, 50C
Archaeoglobus fulgidus
5.4.2.12
3.3
-
3-phospho-D-glycerate
pH 7.0, 50C
Thermoplasma acidophilum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Mg2+
Mn2+ and Mg2+ are required for thermal stability
Archaeoglobus fulgidus
5.4.2.12
Mn2+
in presence of 5 mM Mg2+, 2-3fold stimulation by 0.05 mM Mn2+. Mn2+ and Mg2+ are required for thermal stability
Archaeoglobus fulgidus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
23800
-
2 * 23800, calculated, 2 * 24000, SDS-PAGE
Thermoplasma acidophilum
5.4.2.12
24000
-
2 * 23800, calculated, 2 * 24000, SDS-PAGE
Thermoplasma acidophilum
5.4.2.12
44100
-
1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein
Archaeoglobus fulgidus
5.4.2.12
46000
-
gel filtration
Archaeoglobus fulgidus
5.4.2.12
46000
-
gel filtration
Thermoplasma acidophilum
5.4.2.12
48000
-
1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein
Archaeoglobus fulgidus
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
inhibition of enzyme by vanadate may be partially reversed by EDTA
Thermoplasma acidophilum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.4.2.12
3.3
-
pH 7.0, 50C
Archaeoglobus fulgidus
5.4.2.12
12
-
pH 7.0, 50C
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
679715
Thermoplasma acidophilum
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
679715
Archaeoglobus fulgidus
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
679715
Thermoplasma acidophilum
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
679715
Archaeoglobus fulgidus
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.2.12
dimer
2 * 23800, calculated, 2 * 24000, SDS-PAGE
Thermoplasma acidophilum
5.4.2.12
monomer
1 * 44100, calculated, 1 * 48000, SDS-PAGE of His-tagged protein
Archaeoglobus fulgidus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
5.4.2.12
60
-
120 min, no loss of activity
Archaeoglobus fulgidus
5.4.2.12
70
-
half-life 150 min in presence of Mn2+ and Mg2+. Half-life 30 min in the absence of Mn2+ and Mg2+
Archaeoglobus fulgidus
5.4.2.12
85
-
half-life 15 min in presence of Mn2+ and Mg2+
Archaeoglobus fulgidus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
7.1
-
-
Archaeoglobus fulgidus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.4.2.12
6
-
about 40% of maximal activity
Archaeoglobus fulgidus
5.4.2.12
7.7
-
about 70% of maximal activity
Archaeoglobus fulgidus