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Literature summary extracted from

  • Marcilla, A.; Perez-Garcia, A.; Espert, A.; Bernal, D.; Munoz-Antoli, C.; Esteban, J.G.; Toledo, R.
    Echinostoma caproni: identification of enolase in excretory/secretory products, molecular cloning, and functional expression (2007), Exp. Parasitol., 117, 57-64.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expressed in Escherichia coli strains M15 and SG13009 using expression vector pQE30, protein of 431 amino acids identified, homologies to other trematode enolases estimated Echinostoma caproni

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.11 cytosol
-
 Echinostoma caproni 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 46000
-
 gel filtration, induced expression of the recombinant protein in Escherichia coli Echinostoma caproni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Echinostoma caproni
-
 phosphoenolpyruvate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Echinostoma caproni A1J8J9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 recombinant protein Echinostoma caproni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 intestine of infected hosts Mesocricetus auratus and Rattus norvegicus, excretory and secretory products after infection analyzed Echinostoma caproni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 additional information
-
 binding studies of recombinant protein to human plasminogen confirms properties as host-interacting molecule Echinostoma caproni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Echinostoma caproni phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 2-phospho-D-glycerate studies on host-trematode relationships, properties of enolase as a host-interacting molecule analyzed Echinostoma caproni phosphoenolpyruvate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 enolase
-
 Echinostoma caproni