BRENDA - Enzyme Database

Echinostoma caproni: identification of enolase in excretory/secretory products, molecular cloning, and functional expression

Marcilla, A.; Perez-Garcia, A.; Espert, A.; Bernal, D.; Munoz-Antoli, C.; Esteban, J.G.; Toledo, R.; Exp. Parasitol. 117, 57-64 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli strains M15 and SG13009 using expression vector pQE30, protein of 431 amino acids identified, homologies to other trematode enolases estimated
Echinostoma caproni
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
-
Echinostoma caproni
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46000
-
gel filtration, induced expression of the recombinant protein in Escherichia coli
Echinostoma caproni
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Echinostoma caproni
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Echinostoma caproni
A1J8J9
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
recombinant protein
Echinostoma caproni
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
intestine
of infected hosts Mesocricetus auratus and Rattus norvegicus, excretory and secretory products after infection analyzed
Echinostoma caproni
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
binding studies of recombinant protein to human plasminogen confirms properties as host-interacting molecule
Echinostoma caproni
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
679701
Echinostoma caproni
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
studies on host-trematode relationships, properties of enolase as a host-interacting molecule analyzed
679701
Echinostoma caproni
phosphoenolpyruvate + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli strains M15 and SG13009 using expression vector pQE30, protein of 431 amino acids identified, homologies to other trematode enolases estimated
Echinostoma caproni
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
-
Echinostoma caproni
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46000
-
gel filtration, induced expression of the recombinant protein in Escherichia coli
Echinostoma caproni
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Echinostoma caproni
-
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
recombinant protein
Echinostoma caproni
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
intestine
of infected hosts Mesocricetus auratus and Rattus norvegicus, excretory and secretory products after infection analyzed
Echinostoma caproni
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
binding studies of recombinant protein to human plasminogen confirms properties as host-interacting molecule
Echinostoma caproni
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
679701
Echinostoma caproni
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
studies on host-trematode relationships, properties of enolase as a host-interacting molecule analyzed
679701
Echinostoma caproni
phosphoenolpyruvate + H2O
-
-
-
?