BRENDA - Enzyme Database

Leishmania mexicana: molecular cloning and characterization of enolase

Quinones, W.; Pena, P.; Domingo-Sananes, M.; Caceres, A.; Michels, P.A.; Avilan, L.; Concepcion, J.L.; Exp. Parasitol. 116, 241-251 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
isolated from a genomic library, over-expressed in Escherichia coli, expression vector pET28a
Leishmania mexicana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
diphosphate
inhibits natural enolase and recombinant protein
Leishmania mexicana
4.2.1.11
Mg2+
above 2 mM
Leishmania mexicana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.05
-
2-phospho-D-glycerate
recombinant protein
Leishmania mexicana
4.2.1.11
0.08
-
2-phospho-D-glycerate
natural enolase
Leishmania mexicana
4.2.1.11
0.2
-
phosphoenolpyruvate
recombinant protein
Leishmania mexicana
4.2.1.11
0.21
-
phosphoenolpyruvate
natural enolase
Leishmania mexicana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
enzyme activity only in cytosolic fraction, inactive form detected in the microsomal fraction, membrane-association and localization at the external face of the plasma membrane determined by digitonin treatment and immunofluorescence studies
Leishmania mexicana
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
activity depends on, optimal concentration for activity between 1 mM and 2 mM
Leishmania mexicana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
SDS-PAGE
Leishmania mexicana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Leishmania mexicana
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Leishmania mexicana AZV
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Leishmania mexicana
Q3HL75
-
-
4.2.1.11
Leishmania mexicana AZV
Q3HL75
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
recombinant protein and natural enolase
Leishmania mexicana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
promastigote
-
Leishmania mexicana
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
enolase activity measured spectrophotometrically by following the change in phosphoenolpyruvate concentration, substrate concentrations up to 0.3 mM for 2-phospho-D-glycerate and up to 2 mM for phosphoenolpyruvate
Leishmania mexicana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
679699
Leishmania mexicana
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
679699
Leishmania mexicana AZV
phosphoenolpyruvate + H2O
-
-
-
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
25
-
activity assay at
Leishmania mexicana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.5
-
activity assay at
Leishmania mexicana
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
0.127
-
diphosphate
recombinant protein, substrate 2-phospho-D-glycerate
Leishmania mexicana
4.2.1.11
0.232
-
diphosphate
natural enolase, substrate 2-phospho-D-glycerate
Leishmania mexicana
4.2.1.11
0.238
-
diphosphate
natural enolase, substrate phosphoenolpyruvate
Leishmania mexicana
4.2.1.11
0.26
-
diphosphate
recombinant protein, substrate 2-phospho-D-glycerate
Leishmania mexicana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
isolated from a genomic library, over-expressed in Escherichia coli, expression vector pET28a
Leishmania mexicana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
diphosphate
inhibits natural enolase and recombinant protein
Leishmania mexicana
4.2.1.11
Mg2+
above 2 mM
Leishmania mexicana
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
0.127
-
diphosphate
recombinant protein, substrate 2-phospho-D-glycerate
Leishmania mexicana
4.2.1.11
0.232
-
diphosphate
natural enolase, substrate 2-phospho-D-glycerate
Leishmania mexicana
4.2.1.11
0.238
-
diphosphate
natural enolase, substrate phosphoenolpyruvate
Leishmania mexicana
4.2.1.11
0.26
-
diphosphate
recombinant protein, substrate 2-phospho-D-glycerate
Leishmania mexicana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.05
-
2-phospho-D-glycerate
recombinant protein
Leishmania mexicana
4.2.1.11
0.08
-
2-phospho-D-glycerate
natural enolase
Leishmania mexicana
4.2.1.11
0.2
-
phosphoenolpyruvate
recombinant protein
Leishmania mexicana
4.2.1.11
0.21
-
phosphoenolpyruvate
natural enolase
Leishmania mexicana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
enzyme activity only in cytosolic fraction, inactive form detected in the microsomal fraction, membrane-association and localization at the external face of the plasma membrane determined by digitonin treatment and immunofluorescence studies
Leishmania mexicana
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
activity depends on, optimal concentration for activity between 1 mM and 2 mM
Leishmania mexicana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
SDS-PAGE
Leishmania mexicana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Leishmania mexicana
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Leishmania mexicana AZV
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
recombinant protein and natural enolase
Leishmania mexicana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
promastigote
-
Leishmania mexicana
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
enolase activity measured spectrophotometrically by following the change in phosphoenolpyruvate concentration, substrate concentrations up to 0.3 mM for 2-phospho-D-glycerate and up to 2 mM for phosphoenolpyruvate
Leishmania mexicana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
679699
Leishmania mexicana
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
679699
Leishmania mexicana AZV
phosphoenolpyruvate + H2O
-
-
-
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
25
-
activity assay at
Leishmania mexicana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.5
-
activity assay at
Leishmania mexicana