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Literature summary extracted from
- Leishmania mexicana: molecular cloning and characterization of enolase (2007), Exp. Parasitol., 116, 241-251.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | isolated from a genomic library, over-expressed in Escherichia coli, expression vector pET28a | Leishmania mexicana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.11 | diphosphate | inhibits natural enolase and recombinant protein | Leishmania mexicana |  |
| 4.2.1.11 | Mg2+ | above 2 mM | Leishmania mexicana | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.11 | 0.05 | - |
2-phospho-D-glycerate | recombinant protein | Leishmania mexicana | |
| 4.2.1.11 | 0.08 | - |
2-phospho-D-glycerate | natural enolase | Leishmania mexicana | |
| 4.2.1.11 | 0.2 | - |
phosphoenolpyruvate | recombinant protein | Leishmania mexicana | |
| 4.2.1.11 | 0.21 | - |
phosphoenolpyruvate | natural enolase | Leishmania mexicana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.11 | cytosol | enzyme activity only in cytosolic fraction, inactive form detected in the microsomal fraction, membrane-association and localization at the external face of the plasma membrane determined by digitonin treatment and immunofluorescence studies | Leishmania mexicana | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.11 | Mg2+ | activity depends on, optimal concentration for activity between 1 mM and 2 mM | Leishmania mexicana | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 50000 | - |
SDS-PAGE | Leishmania mexicana |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.11 | 2-phospho-D-glycerate | Leishmania mexicana | - |
phosphoenolpyruvate + H2O | - |
r | |
| 4.2.1.11 | 2-phospho-D-glycerate | Leishmania mexicana AZV | - |
phosphoenolpyruvate + H2O | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.11 | Leishmania mexicana | Q3HL75 | - |
- |
| 4.2.1.11 | Leishmania mexicana AZV | Q3HL75 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | recombinant protein and natural enolase | Leishmania mexicana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.1.11 | promastigote | - |
Leishmania mexicana | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | additional information | - |
enolase activity measured spectrophotometrically by following the change in phosphoenolpyruvate concentration, substrate concentrations up to 0.3 mM for 2-phospho-D-glycerate and up to 2 mM for phosphoenolpyruvate | Leishmania mexicana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Leishmania mexicana | phosphoenolpyruvate + H2O | - |
r | |
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Leishmania mexicana AZV | phosphoenolpyruvate + H2O | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | enolase | - |
Leishmania mexicana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 25 | - |
activity assay at | Leishmania mexicana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 7.5 | - |
activity assay at | Leishmania mexicana |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.11 | 0.127 | - |
diphosphate | recombinant protein, substrate 2-phospho-D-glycerate | Leishmania mexicana | |
| 4.2.1.11 | 0.232 | - |
diphosphate | natural enolase, substrate 2-phospho-D-glycerate | Leishmania mexicana | |
| 4.2.1.11 | 0.238 | - |
diphosphate | natural enolase, substrate phosphoenolpyruvate | Leishmania mexicana | |
| 4.2.1.11 | 0.26 | - |
diphosphate | recombinant protein, substrate 2-phospho-D-glycerate | Leishmania mexicana | |
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