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Literature summary extracted from

  • Consalvi, V.; Chiaraluce, R.; Politi, L.; Gambacorta, A.; De Rosa, M.; Scandurra, R.
    Glutamate dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus. (1991), Eur. J. Biochem., 196, 459-467.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.3 0.01
-
 NADPH pH 7.6, 60°C Saccharolobus solfataricus
1.4.1.3 0.14
-
 NADH pH 7.6, 50°C Saccharolobus solfataricus
1.4.1.3 1.4
-
 2-oxoglutarate pH 7.6, 60°C Saccharolobus solfataricus
1.4.1.3 2.5
-
 L-glutamate pH 7.6, 60°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 45000
-
 6 * 45000, SDS-PAGE Saccharolobus solfataricus
1.4.1.3 270000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 35
-
-
 Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
1.4.1.3 4°C, 1 mg/ml protein concentration, glutamate dehydrogenase remains stable for at least one month Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 2-oxoglutarate + NH3 + NADH specific activity in the presence of NADH is about 30% of that with NADPH Saccharolobus solfataricus L-glutamate + H2O + NAD+
-
 r
1.4.1.3 2-oxoglutarate + NH3 + NADPH
-
 Saccharolobus solfataricus L-glutamate + H2O + NADP+
-
 r
1.4.1.3 L-glutamate + H2O + NADP+
-
 Saccharolobus solfataricus 2-oxoglutarate + NH3 + NADPH
-
 r
1.4.1.3 norvaline + H2O + NADP+ activity is 20% of that observed in the presence of 2-oxoglutarate and L-glutamate Saccharolobus solfataricus 2-oxovalerate + NH3 + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.3 hexamer 6 * 45000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.4.1.3 NAD(P)-dependent glutamate dehydrogenase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.3 65
-
 substrates: 2-oxoglutarate + NH3 + NADPH Saccharolobus solfataricus
1.4.1.3 70
-
 substrates: L-glutamate + H2O + NADP+ Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.3 45 75 45°C: about 35% of maximal activity, 75°C: about 20% of maximal activity, substrates: 2-oxoglutarate + NH3 + NADPH Saccharolobus solfataricus
1.4.1.3 60 75 60°C: about 60% of maximal activity, 75°C: about 30% of maximal activity, substrates: L-glutamate + H2O + NADP+ Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.3 25
-
 48 h, protein concentration of 0.2 mg/ml, stable Saccharolobus solfataricus
1.4.1.3 60
-
 48 h, protein concentration of 0.2 mg/ml, 20% loss of activity Saccharolobus solfataricus
1.4.1.3 80
-
 half-life: 15 h Saccharolobus solfataricus
1.4.1.3 85
-
 half-life: 2 h Saccharolobus solfataricus
1.4.1.3 90
-
 half-life: 20 min Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.3 9
-
 substrates: 2-oxoglutarate + NH3 + NADPH Saccharolobus solfataricus
1.4.1.3 10
-
 substrates: L-glutamate + H2O + NADP+ Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.3 NADH
-
 Saccharolobus solfataricus
1.4.1.3 NADP+
-
 Saccharolobus solfataricus
1.4.1.3 NADPH
-
 Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.1.3 Saccharolobus solfataricus isoelectric focusing. Microheterogeneity shows three active bands corresponding to pI of 5.7, 5.5 and 5.4. The major band shows an isoelectric point of 5.7
-
 5.7