EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.3 | 0.01 | - |
NADPH | pH 7.6, 60°C | Saccharolobus solfataricus | |
1.4.1.3 | 0.14 | - |
NADH | pH 7.6, 50°C | Saccharolobus solfataricus | |
1.4.1.3 | 1.4 | - |
2-oxoglutarate | pH 7.6, 60°C | Saccharolobus solfataricus | |
1.4.1.3 | 2.5 | - |
L-glutamate | pH 7.6, 60°C | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.3 | 45000 | - |
6 * 45000, SDS-PAGE | Saccharolobus solfataricus |
1.4.1.3 | 270000 | - |
gel filtration | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.3 | Saccharolobus solfataricus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.3 | - |
Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.3 | 35 | - |
- |
Saccharolobus solfataricus |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.3 | 4°C, 1 mg/ml protein concentration, glutamate dehydrogenase remains stable for at least one month | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.3 | 2-oxoglutarate + NH3 + NADH | specific activity in the presence of NADH is about 30% of that with NADPH | Saccharolobus solfataricus | L-glutamate + H2O + NAD+ | - |
r | |
1.4.1.3 | 2-oxoglutarate + NH3 + NADPH | - |
Saccharolobus solfataricus | L-glutamate + H2O + NADP+ | - |
r | |
1.4.1.3 | L-glutamate + H2O + NADP+ | - |
Saccharolobus solfataricus | 2-oxoglutarate + NH3 + NADPH | - |
r | |
1.4.1.3 | norvaline + H2O + NADP+ | activity is 20% of that observed in the presence of 2-oxoglutarate and L-glutamate | Saccharolobus solfataricus | 2-oxovalerate + NH3 + NADPH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.3 | hexamer | 6 * 45000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.3 | NAD(P)-dependent glutamate dehydrogenase | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.3 | 65 | - |
substrates: 2-oxoglutarate + NH3 + NADPH | Saccharolobus solfataricus |
1.4.1.3 | 70 | - |
substrates: L-glutamate + H2O + NADP+ | Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.3 | 45 | 75 | 45°C: about 35% of maximal activity, 75°C: about 20% of maximal activity, substrates: 2-oxoglutarate + NH3 + NADPH | Saccharolobus solfataricus |
1.4.1.3 | 60 | 75 | 60°C: about 60% of maximal activity, 75°C: about 30% of maximal activity, substrates: L-glutamate + H2O + NADP+ | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.3 | 25 | - |
48 h, protein concentration of 0.2 mg/ml, stable | Saccharolobus solfataricus |
1.4.1.3 | 60 | - |
48 h, protein concentration of 0.2 mg/ml, 20% loss of activity | Saccharolobus solfataricus |
1.4.1.3 | 80 | - |
half-life: 15 h | Saccharolobus solfataricus |
1.4.1.3 | 85 | - |
half-life: 2 h | Saccharolobus solfataricus |
1.4.1.3 | 90 | - |
half-life: 20 min | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.3 | 9 | - |
substrates: 2-oxoglutarate + NH3 + NADPH | Saccharolobus solfataricus |
1.4.1.3 | 10 | - |
substrates: L-glutamate + H2O + NADP+ | Saccharolobus solfataricus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.3 | NADH | - |
Saccharolobus solfataricus | |
1.4.1.3 | NADP+ | - |
Saccharolobus solfataricus | |
1.4.1.3 | NADPH | - |
Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.4.1.3 | Saccharolobus solfataricus | isoelectric focusing. Microheterogeneity shows three active bands corresponding to pI of 5.7, 5.5 and 5.4. The major band shows an isoelectric point of 5.7 | - |
5.7 |