Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pisani, F.M.; Rella, R.; Raia, C.A.; Rozzo, C.; Nucci, R.; Gambacorta, A.; De Rosa, M.; Rossi, M.
    Thermostable beta-galactosidase from the archaebacterium Sulfolobus solfataricus. Purification and properties (1990), Eur. J. Biochem., 187, 321-328.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 2-mercaptoethanol slight activation Saccharolobus solfataricus
3.2.1.23 cysteine slight activation Saccharolobus solfataricus
3.2.1.23 dithiothreitol slight activation Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 4-aminophenyl beta-D-thiogalactopyranoside competitive inhibition Saccharolobus solfataricus
3.2.1.23 beta-D-glucose product inhibition Saccharolobus solfataricus
3.2.1.23 D-galactal
-
 Saccharolobus solfataricus
3.2.1.23 additional information no inhibition by 4-chloromercuribenzoate or N-ethylmaleimide, and by D-galactose and isopropyl beta-D-thiogalactopyranoside Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 additional information
-
 additional information kinetics and thermodynamics Saccharolobus solfataricus
3.2.1.23 0.225
-
 2-nitrophenyl-beta-D-galactopyranoside pH 6.5, 75°C Saccharolobus solfataricus
3.2.1.23 2 3 methyl-beta-D-galactopyranoside pH 6.5, 30°C Saccharolobus solfataricus
3.2.1.23 2.6
-
 phenyl-beta-D-galactopyranoside pH 6.5, 30°C Saccharolobus solfataricus
3.2.1.23 13
-
 lactose pH 6.5, 30°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 60000
-
 4 * 60000, SDS-PAGE Saccharolobus solfataricus
3.2.1.23 220000
-
 glycerol gradient centrifugation Saccharolobus solfataricus
3.2.1.23 240000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Saccharolobus solfataricus
-
-
-
3.2.1.23 Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 soluble, native enzyme 966fold by anion exchange chromatography, precipitation at pH 4.5, dialysis, 4-aminophenyl-beta-D-thiogalactopyranoside affinity chromatography, and another step of anion exchange chromatography to homogeneity Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 116.4
-
 purified enzyme Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
3.2.1.23 -20°C, purified enzyme, 50 mM sodium phosphate, pH 6.5, and 2.8 mM 4-nitrophenyl-beta-D-galactopyranoside, stable for long periods of storage Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Saccharolobus solfataricus beta-D-glucose + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 beta-D-glucose + beta-D-galactose
-
 ?
3.2.1.23 methyl-beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus methanol + beta-D-galactose
-
 ?
3.2.1.23 methyl-beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 methanol + beta-D-galactose
-
 ?
3.2.1.23 additional information the enzyme displays a quite broad specificity, showing wide tolerance regarding the structure of the aglycon moiety, overview Saccharolobus solfataricus ?
-
 ?
3.2.1.23 additional information the enzyme displays a quite broad specificity, showing wide tolerance regarding the structure of the aglycon moiety, overview Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
 ?
3.2.1.23 phenyl-beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus phenol + beta-D-galactose
-
 ?
3.2.1.23 phenyl-beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 phenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 More enzyme amino acid composition, overview Saccharolobus solfataricus
3.2.1.23 tetramer 4 * 60000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 95
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 35 95 temperature profile, 3% of maximal activity at 30°C Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 75
-
 purified enzyme, pH 6.5, half-life is 24 h Saccharolobus solfataricus
3.2.1.23 80
-
 purified enzyme, pH 6.5, half-life is 10 h Saccharolobus solfataricus
3.2.1.23 85
-
 purified enzyme, pH 6.5, half-life is 3 h Saccharolobus solfataricus
3.2.1.23 95
-
 purified enzyme, pH 6.5, complete loss of activity after 120 min Saccharolobus solfataricus
3.2.1.23 100
-
 purified enzyme, pH 6.5, complete loss of activity after 15 min Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 114
-
 lactose pH 6.5, 30°C Saccharolobus solfataricus
3.2.1.23 166
-
 phenyl-beta-D-galactopyranoside pH 6.5, 30°C Saccharolobus solfataricus
3.2.1.23 242
-
 methyl-beta-D-galactopyranoside pH 6.5, 30°C Saccharolobus solfataricus
3.2.1.23 5700
-
 2-nitrophenyl-beta-D-galactopyranoside pH 6.5, 75°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.5
-
 assay at Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 3.5 10 pH profile Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.23 4
-
 4-aminophenyl beta-D-thiogalactopyranoside pH 6.5, 75°C Saccharolobus solfataricus
3.2.1.23 12
-
 D-galactal pH 6.5, 75°C Saccharolobus solfataricus
3.2.1.23 96
-
 beta-D-glucose pH 6.5, 75°C Saccharolobus solfataricus