EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.26 | expressed in Escherichia coli and in an invertase-negative strain of Saccharomyces cerevisiae | Aspergillus niger |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.26 | sucrose | increase of the sucrose concentration from 2.5 mM to 1 M results in decreased hydrolysis activity | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.26 | 2 | - |
sucrose | in 50 mM acetate buffer (pH 5.0) at 37°C | Aspergillus niger |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 75000 | - |
SDS-PAGE | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.26 | Aspergillus niger | Q0ZR36 | putative intracellular invertase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.26 | His tag affinity chromatography | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.26 | 1-kestose + H2O | - |
Aspergillus niger | D-fructose + ? | - |
? | |
3.2.1.26 | additional information | does not use inulin and levan as substrates | Aspergillus niger | ? | - |
? | |
3.2.1.26 | nystose + H2O | - |
Aspergillus niger | D-fructose + ? | - |
? | |
3.2.1.26 | sucrose + H2O | - |
Aspergillus niger | D-glucose + D-fructose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.26 | invertase | - |
Aspergillus niger |
3.2.1.26 | SucB | shows also transfructosylation activity of 20 to 30% of total activity at sucrose concentrations up to 400 mM | Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 37 | 40 | - |
Aspergillus niger |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 25 | 50 | at 50°C or higher, no activity can be detected, whereas at lower temperatures, the SucB specific activity remains relatively high, with 50% activity remaining at 25°C | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 5 | - |
- |
Aspergillus niger |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.26 | 268.1 | - |
sucrose | in 50 mM acetate buffer (pH 5.0) at 37°C | Aspergillus niger |