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Literature summary extracted from

  • Xue, Z.; McCluskey, M.; Cantera, K.; Ben-Bassat, A.; Sariaslani, F.S.; Huang, L.
    Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme (2007), Enzyme Microb. Technol., 42, 58-64.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.25 controlled expression in Escherichia coli to mitigate product toxicity Rhodotorula glutinis
4.3.1.25 controlled expression in Escherichia coli to mitigate product toxicity Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.25 0.044
-
 L-tyrosine pH 9.5, 35°C Phanerodontia chrysosporium
4.3.1.25 0.0677
-
 L-tyrosine pH 9.5 Rhodotorula glutinis
4.3.1.25 0.126
-
 L-phenylalanine pH 9.5 Rhodotorula glutinis
4.3.1.25 0.161
-
 L-phenylalanine pH 9.5, 35°C Phanerodontia chrysosporium
4.3.1.25 0.183
-
 L-phenylalanine pH 8.5 Rhodotorula glutinis
4.3.1.25 0.615
-
 L-tyrosine pH 8.5 Rhodotorula glutinis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.25 70000
-
 x * 70000, SDS-PAGE Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.25 Phanerodontia chrysosporium
-
-
-
4.3.1.25 Rhodotorula glutinis
-
-
-

Storage Stability

EC Number Storage Stability Organism
4.3.1.25 -80°C, 50 mM Tris, pH 8.5, 25% glycerol, stable Phanerodontia chrysosporium
4.3.1.25 4°C, 50 mM Tris, pH 8.5, stable for at least a month Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.25 L-phenylalanine
-
 Rhodotorula glutinis (E)-cinnamate + NH3
-
 ?
4.3.1.25 L-phenylalanine
-
 Phanerodontia chrysosporium (E)-cinnamate + NH3
-
 ?
4.3.1.25 L-tyrosine
-
 Rhodotorula glutinis p-hydroxycinnamic acid + NH3
-
 ?
4.3.1.25 L-tyrosine
-
 Phanerodontia chrysosporium p-hydroxycinnamic acid + NH3
-
 ?
4.3.1.25 additional information no substrate: L-histidine Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.25 ? x * 70000, SDS-PAGE Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.25 60
-
 3 h, no loss of activity, 4 h, 72% residual activity Phanerodontia chrysosporium
4.3.1.25 60
-
 4 h, 10% residual activity Rhodotorula glutinis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.25 0.53
-
 L-tyrosine pH 8.5 Rhodotorula glutinis
4.3.1.25 0.93
-
 L-tyrosine pH 9.5 Rhodotorula glutinis
4.3.1.25 1.3
-
 L-tyrosine pH 9.5, 35°C Phanerodontia chrysosporium
4.3.1.25 1.53
-
 L-phenylalanine pH 9.5 Rhodotorula glutinis
4.3.1.25 1.6
-
 L-phenylalanine pH 8.5 Rhodotorula glutinis
4.3.1.25 3.3
-
 L-phenylalanine pH 9.5, 35°C Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.25 8.5
-
 35°C Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.25 8 9.5 above 88% of maximum activity Phanerodontia chrysosporium
4.3.1.25 10
-
 80% of maximum activity Phanerodontia chrysosporium
4.3.1.25 12
-
 12% of maximum activity Phanerodontia chrysosporium