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Literature summary extracted from

  • Guimaraes, L.H.; Terenzi, H.F.; Polizeli, M.d.; Jorge, J.A.
    Production and characterization of a thermostable extracellular beta-D-fructofuranosidase produced by Aspergillus ochraceus with agroindustrial residues as carbon sources (2007), Enzyme Microb. Technol., 42, 52-57.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.26 D-glucose stimulates activity 2.68fold at 40 mM Aspergillus ochraceus
3.2.1.26 EDTA slightly stimulating at 1 mM Aspergillus ochraceus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 Ca2+ 73% residual activity at 1 mM Aspergillus ochraceus
3.2.1.26 Cu2+ 53% residual activity at 10 mM; 94% residual activity at 1 mM Aspergillus ochraceus
3.2.1.26 Hg+ complete inhibition at 10 mM Aspergillus ochraceus
3.2.1.26 Mg2+ 79% residual activity at 10 mM Aspergillus ochraceus
3.2.1.26 Na+ 73% residual activity at 10 mM Aspergillus ochraceus
3.2.1.26 NH4Cl 25% residual activity at 10 mM Aspergillus ochraceus
3.2.1.26 Zn2+ 19% residual activity at 10 mM Aspergillus ochraceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 2.66
-
 inulin in sodium acetate buffer, 100 mM, pH 4.5, at 60°C Aspergillus ochraceus
3.2.1.26 7.37
-
 raffinose in sodium acetate buffer, 100 mM, pH 4.5, at 60°C Aspergillus ochraceus
3.2.1.26 13.4
-
 sucrose in sodium acetate buffer, 100 mM, pH 4.5, at 60°C Aspergillus ochraceus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.26 Ba2+ 20% stimulation of activity at 1 mM Aspergillus ochraceus
3.2.1.26 Mg2+ 50% stimulation of activity at 1 mM Aspergillus ochraceus
3.2.1.26 Mn2+ 57% stimulation of activity at 1 mM Aspergillus ochraceus
3.2.1.26 Na+ 35% stimulation of activity at 1 mM Aspergillus ochraceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 67500
-
 2 * 67500, gel filtration Aspergillus ochraceus
3.2.1.26 79000
-
 2 * 79000, SDS-PAGE Aspergillus ochraceus
3.2.1.26 135000
-
 gel filtration Aspergillus ochraceus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Aspergillus ochraceus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.26 glycoprotein 41% carbohydrate content Aspergillus ochraceus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 DEAE-cellulose chromatography and Sephacryl S-200 gel filtration Aspergillus ochraceus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 6
-
 crude extract, at pH 4.5, 60°C Aspergillus ochraceus
3.2.1.26 42.5
-
 after 7.1fold purification, at pH 4.5, 60°C Aspergillus ochraceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 inulin + H2O
-
 Aspergillus ochraceus D-fructose + ?
-
 ?
3.2.1.26 raffinose + H2O
-
 Aspergillus ochraceus fructose + melibiose
-
 ?
3.2.1.26 sucrose + H2O
-
 Aspergillus ochraceus D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 homodimer 2 * 79000, SDS-PAGE Aspergillus ochraceus
3.2.1.26 homodimer 2 * 67500, gel filtration Aspergillus ochraceus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 beta-D-fructofuranosidase
-
 Aspergillus ochraceus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 60
-
-
 Aspergillus ochraceus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 60
-
 half-life is 60 min at 60°C Aspergillus ochraceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 4.5
-
-
 Aspergillus ochraceus