Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Takesue, N.; Sone, T.; Tanaka, M.; Tomita, F.; Asano, K.
    Production of di-D-fructofranosyl-2,6:2,6-anhydride (DFA IV) by recombinant Bacillus subtilis carrying heterogenous levan fructotransferase from Arthrobacter nicotinovorans GS-9 (2007), Enzyme Microb. Technol., 41, 673-676.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.2.16 synthesis development of a di-D-fructofranosyl-2,6':2',6-anhydride production system with single culture of Bacillus subtilis directly from sucrose. This system can avoid the purification procedure of levan in which organic solvent is used for precipitation. The levan fructotransferase gene is cloned from Arthrobacter nicotinovorans GS-9 and expressed in levan producing Bacillus subtilis 168. LFTase activity is detected in the culture supernatant of the transformant with maximal activity of 0.062 U/ml after 15.5 h post induction. Then sucrose is added as substrate and incubated. About 78 h after addition of sucrose, 20.5 g/l of di-D-fructofranosyl-2,6':2',6-anhydride is produced from 139.3 g/l of sucrose consumed. The yield of di-D-fructofranosyl-2,6':2',6-anhydride from sucrose is 14.7 wt.% Paenarthrobacter nicotinovorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.16 levan fructotransferase gene is cloned from Arthrobacter nicotinovorans GS-9 and expressed in levan producing Bacillus subtilis 168 Paenarthrobacter nicotinovorans

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.16 Paenarthrobacter nicotinovorans
-
-
-
4.2.2.16 Paenarthrobacter nicotinovorans GS-9
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.16 levan
-
 Paenarthrobacter nicotinovorans 5 di-D-fructose-2,6':2',6 dianhydride + ?
-
 ?
4.2.2.16 levan
-
 Paenarthrobacter nicotinovorans GS-9 5 di-D-fructose-2,6':2',6 dianhydride + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.16 LFTase
-
 Paenarthrobacter nicotinovorans