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Literature summary extracted from

  • Riaz, M.; Perveen, R.; Javed, M.R.; Nadeem, H.; Rashid, M.H.
    Kinetic and thermodynamic properties of novel glucoamylase from Humicola sp. (2007), Enzyme Microb. Technol., 41, 558-564.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information kinetics and thermodynamics Humicola sp.
3.2.1.3 0.26
-
 soluble starch pH 4.7, 55°C Humicola sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular
-
 Humicola sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 72800
-
 gel filtration Humicola sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Humicola sp.
-
 D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Humicola sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 native extracellular enzyme 39fold to homogeneity by ammonium sulfate precipitation, anion exchange chromatography, and hydrophobic interaction chromatography Humicola sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.3 cell culture submerged growth condition Humicola sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 10.44
-
 cell culture Humicola sp.
3.2.1.3 406.9
-
 purified native enzyme Humicola sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 soluble starch + H2O
-
 Humicola sp. beta-D-glucose + ?
-
 ?
3.2.1.3 soluble starch + H2O
-
 Humicola sp. beta-D-glucose + soluble starch
-
 ?
3.2.1.3 starch + H2O
-
 Humicola sp. D-glucose + ?
-
 ?
3.2.1.3 starch + H2O soluble starch Humicola sp. D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 monomer 1 * 72800, SDS-PAGE Humicola sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Humicola sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 55
-
-
 Humicola sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 69
-
 soluble starch pH 4.7, 55°C Humicola sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.7
-
-
 Humicola sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 3.5 5.9
-
 Humicola sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 3.5 5.9 purified native extracellular enzyme, stable Humicola sp.