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Literature summary extracted from

  • Peng, S.S.; Deng, M.; Grund, A.D.; Rosson, R.A.
    Purification and characterization of a membrane-bound linoleic acid isomerase from Clostridium sporogenes (2007), Enzyme Microb. Technol., 40, 831-839.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.2.1.5 additional information no effects by DTT, ATP, ADP, NAD+, NADH, NADPH or CoA Clostridium sporogenes

General Stability

EC Number General Stability Organism
5.2.1.5 the enzyme is very unstable, especially after being solubilized by detergents, increasing glycerol concentration to 30% and salt concentration to 0.5 M improves isomerase stability, resulting in a near full retention of activity in crude extracts for 1 week Clostridium sporogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.2.1.5 9-cis,12-cis-linoleic acid methyl ester i.e. methyl linoleate, 31% inhibition at 0.02 mM Clostridium sporogenes
5.2.1.5 9-cis,12-cis-octadecadien-1-ol i.e. linoleyl alcohol, 19% inhibition at 0.02 mM Clostridium sporogenes
5.2.1.5 gamma-linolenic acid substrate inhibition at concentrations above 0.015 mM Clostridium sporogenes
5.2.1.5 linoleic acid substrate inhibition at concentrations above 0.02 mM, some linoleic acid derivatives also inhibited the isomerase Clostridium sporogenes
5.2.1.5 linolelaidic acid 81% inhibition at 0.02 mM Clostridium sporogenes
5.2.1.5 linolenic acid substrate inhibition at concentrations above 0.04 mM Clostridium sporogenes
5.2.1.5 additional information no effects by DTT, ATP, ADP, NAD+, NADH, NADPH or CoA Clostridium sporogenes
5.2.1.5 oleic acid 75% inhibition at 0.02 mM Clostridium sporogenes
5.2.1.5 palmitoleic acid 72% inhibition at 0.02 mM Clostridium sporogenes
5.2.1.5 stearidonic acid substrate inhibition at concentrations above 0.120 mM Clostridium sporogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2.1.5 additional information
-
additional information
-
Clostridium sporogenes
5.2.1.5 0.0034
-
6-cis,9-cis,12-cis,15-cis-octadecatetraenoic acid pH 7.5, 22°C Clostridium sporogenes
5.2.1.5 0.0077
-
9-cis,12-cis,15-cis-octadecatrienoic acid pH 7.5, 22°C Clostridium sporogenes
5.2.1.5 0.0119
-
9-cis,12-cis-linoleic acid pH 7.5, 22°C Clostridium sporogenes
5.2.1.5 0.0258
-
6-cis,9-cis,12-cis-octadecatrienoic acid pH 7.5, 22°C Clostridium sporogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.2.1.5 membrane bound Clostridium sporogenes 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.2.1.5 45000
-
4 * 45000, SDS-PAGE Clostridium sporogenes
5.2.1.5 190000
-
native PAGE Clostridium sporogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.2.1.5 9-cis,11-trans-linoleic acid Clostridium sporogenes formation only under aerobic conditions 9-trans,11-trans-linoleic acid
-
?
5.2.1.5 9-cis,12-cis-linoleic acid Clostridium sporogenes higher activity under aerobic than under anaerobic conditions 9-cis,11-trans-linoleic acid
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.5 Clostridium sporogenes
-
strain ATCC 25762
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.2.1.5 native enzyme 364fold by anion exchange chromatography, chromatofocusing, and gel filtration, method optimization for purification of the very unstable enzyme, overview Clostridium sporogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.2.1.5 0.4
-
purified enzyme Clostridium sporogenes

Storage Stability

EC Number Storage Stability Organism
5.2.1.5 the enzyme is very unstable, especially after being solubilized by detergents, increasing glycerol concentration to 30% and salt concentration to 0.5 M improves isomerase stability, resulting in a near full retention of activity in crude extracts for 1 week Clostridium sporogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.5 11-cis,14-cis-eicosadienoic acid
-
Clostridium sporogenes ?
-
?
5.2.1.5 6-cis,9-cis,12-cis,15-cis-octadecatetraenoic acid i.e. stearidonic acid Clostridium sporogenes ?
-
?
5.2.1.5 6-cis,9-cis,12-cis-octadecatrienoic acid i.e. alpha-linolenic acid Clostridium sporogenes ?
-
?
5.2.1.5 9-cis,11-trans-linoleic acid formation only under aerobic conditions Clostridium sporogenes 9-trans,11-trans-linoleic acid
-
?
5.2.1.5 9-cis,12-cis,15-cis-octadecatrienoic acid i.e. gamma-linolenic acid Clostridium sporogenes ?
-
?
5.2.1.5 9-cis,12-cis-linoleic acid higher activity under aerobic than under anaerobic conditions Clostridium sporogenes 9-cis,11-trans-linoleic acid
-
?
5.2.1.5 9-cis,12-cis-linoleic acid higher activity under aerobic than under anaerobic conditions Clostridium sporogenes 9-cis,11-trans-linoleic acid conjugated linoleic acid isomer ?
5.2.1.5 additional information substrate specificity, overview, the enzyme is specific for substrates containing cis double bonds at the c9 and c12 positions of C18 polyunsaturated fatty acids, a free carboxyl group is absolutely necessary for isomerization Clostridium sporogenes ?
-
?

Subunits

EC Number Subunits Comment Organism
5.2.1.5 tetramer 4 * 45000, SDS-PAGE Clostridium sporogenes

Synonyms

EC Number Synonyms Comment Organism
5.2.1.5 Linoleic acid isomerase
-
Clostridium sporogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.2.1.5 22
-
assay at room temperature Clostridium sporogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2.1.5 7.5
-
-
Clostridium sporogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.2.1.5 5.7 9
-
Clostridium sporogenes

Cofactor

EC Number Cofactor Comment Organism Structure
5.2.1.5 additional information no external cofactors are required for catalysis Clostridium sporogenes